Implication of metastasis suppressor gene, Kiss-1 and its receptor Kiss-1R in colorectal cancer.

Ji K, Ye L, Ruge F, Hargest R, Mason MD, Jiang WG

BMC cancer27 Sept 2014Cited by 28 (Europe PMC)

PMID25260785PMCIDPMC4190326DOI10.1186/1471-2407-14-723PepBankPUB-25260785

Summary

BackgroundKiss-1 and Kiss-1R have been suggested as a novel pair of metastasis suppressors for several human solid tumours, however, their role in colorectal cancer remains largely unknown. Therefore, the aim of this study was to investigate the role and signal transduction of Kiss-1 and Kiss-1R in colorectal cancer.MethodsRibozyme transgenes were used to knockdown high expression of Kiss-1 and Kiss-1R in HT115 and HRT18 cells. The stabilized transfected cells were then used to deduce the influence of Kiss-1 and Kiss-1R on the function of colorectal cancer cells by in vitro assays and ECIS assay. The effect of Kiss-1 on MMPs related to tumour metastasis was also deleted by zymography. The mRNA and protein expression of Kiss-1 and Kiss-1R, and their correlation to the clinical outcome in human colorectal cancer were investigated using real-time PCR and IHC respectively.ResultsKnocking down Kiss-1 resulted in increased invasion and migration of colorectal cancer cells. Kisspeptin-10 decreased cellular migration of colorectal cancer cells and required ERK signaling as shown during the ECIS based analyses. Reduction of MMP-9 was caused by Kisspeptin-10 and ERK inhibitor, shown by zymography. In human colorectal cancer tissues, the mRNA expression level of Kiss-1 had a negative correlation with Dukes staging, TNM staging, tumour size and lymph node involvement. Reduction of Kiss-1R was also linked to poor prognosis for the patients.ConclusionsThe present study has presented evidence that Kiss-1 may be a putative metastasis suppressor molecule in human colorectal cancer.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study9

3 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In colorectal cancer cells, kisspeptin‑10 decreased cell migration.
In plain wordsIn cancer cells in the lab, kisspeptin‑10 reduced the movement of colorectal cancer cells.
Peptide
Kisspeptin-10
Outcome
DecreaseColorectal cancer cell migration
Study
humann: not reported in source
Colorectal cancer cells
Outcome group
Tumor growth · Cancer
Studied dose
Dose not stated
Source sentences
Summary
Kisspeptin-10 decreased cellular migration of colorectal cancer cells
1 context sentence(design, population, supporting numbers)
Summary · context
The stabilized transfected cells were then used to deduce the influence of Kiss-1 and Kiss-1R on the function of colorectal cancer cells by in vitro assays and ECIS assay.
Show in the study text Report a problemFND-2526078501
02LabIn vitro (cells)Mechanism
In colorectal cancer cells, the kisspeptin‑10 effect on migration required ERK signaling.
In plain wordsIn cancer cells in the lab, the effect of kisspeptin‑10 on cell movement needed ERK signaling.
Peptide
Kisspeptin-10
Outcome
Not applicableERK dependence of kisspeptin-10 effect on migration
Study
humann: not reported in source
Colorectal cancer cells
Outcome group
Tumor growth · Cancer
Studied dose
Dose not stated
Source sentences
Summary
and required ERK signaling as shown during the ECIS based analyses.
1 context sentence(design, population, supporting numbers)
Summary · context
The stabilized transfected cells were then used to deduce the influence of Kiss-1 and Kiss-1R on the function of colorectal cancer cells by in vitro assays and ECIS assay.
Show in the study text Report a problemFND-2526078502
03LabIn vitro (cells)Mechanism
In colorectal cancer cells, zymography showed reduced MMP-9 with kisspeptin‑10 and ERK inhibitor treatment.
In plain wordsIn cancer cells in the lab, MMP-9, an enzyme linked to cancer spread, was lower after treatment with kisspeptin‑10 and an ERK blocker.
Peptide
Kisspeptin-10
Outcome
DecreaseMMP-9 level by zymography
Study
humann: not reported in source
Colorectal cancer cells
Outcome group
Tumor growth · Cancer
Studied dose
Dose not stated
Source sentences
Summary
Reduction of MMP-9 was caused by Kisspeptin-10 and ERK inhibitor, shown by zymography.
1 context sentence(design, population, supporting numbers)
Summary · context
The effect of Kiss-1 on MMPs related to tumour metastasis was also deleted by zymography.
Show in the study text Report a problemFND-2526078503

Natural levels measured4

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

04HumanEndogenous levelObservational studyOther
In human colorectal cancer tissues, Kiss-1 mRNA expression correlated negatively with Dukes staging.
In plain wordsIn people with colorectal cancer, lower levels of the body's own Kiss-1 gene activity were linked to higher Dukes stage.
Peptide
Kisspeptin-10
Outcome
Not applicableKiss-1 mRNA level and Dukes staging
Study
n: not reported in source
Outcome group
Tumor growth · Cancer
Source sentences
Summary
In human colorectal cancer tissues, the mRNA expression level of Kiss-1 had a negative correlation with Dukes staging, TNM staging, tumour size and lymph node involvement.
1 context sentence(design, population, supporting numbers)
Summary · context
The mRNA and protein expression of Kiss-1 and Kiss-1R, and their correlation to the clinical outcome in human colorectal cancer were investigated using real-time PCR and IHC respectively.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2526078506
05HumanEndogenous levelObservational studyOther
In human colorectal cancer tissues, Kiss-1 mRNA expression correlated negatively with TNM staging.
In plain wordsIn people with colorectal cancer, lower levels of the body's own Kiss-1 gene activity were linked to higher TNM stage.
Peptide
Kisspeptin-10
Outcome
Not applicableKiss-1 mRNA level and TNM staging
Study
n: not reported in source
Outcome group
Tumor growth · Cancer
Source sentences
Summary
In human colorectal cancer tissues, the mRNA expression level of Kiss-1 had a negative correlation with Dukes staging, TNM staging, tumour size and lymph node involvement.
1 context sentence(design, population, supporting numbers)
Summary · context
The mRNA and protein expression of Kiss-1 and Kiss-1R, and their correlation to the clinical outcome in human colorectal cancer were investigated using real-time PCR and IHC respectively.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2526078507
06HumanEndogenous levelObservational studyOther
In human colorectal cancer tissues, Kiss-1 mRNA expression correlated negatively with tumour size.
In plain wordsIn people with colorectal cancer, lower levels of the body's own Kiss-1 gene activity were linked to larger tumours.
Peptide
Kisspeptin-10
Outcome
Not applicableKiss-1 mRNA level and tumour size
Study
n: not reported in source
Outcome group
Tumor growth · Cancer
Source sentences
Summary
In human colorectal cancer tissues, the mRNA expression level of Kiss-1 had a negative correlation with Dukes staging, TNM staging, tumour size and lymph node involvement.
1 context sentence(design, population, supporting numbers)
Summary · context
The mRNA and protein expression of Kiss-1 and Kiss-1R, and their correlation to the clinical outcome in human colorectal cancer were investigated using real-time PCR and IHC respectively.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2526078508
07HumanEndogenous levelObservational studyOther
In human colorectal cancer tissues, Kiss-1 mRNA expression correlated negatively with lymph node involvement.
In plain wordsIn people with colorectal cancer, lower levels of the body's own Kiss-1 gene activity were linked to more lymph node involvement.
Peptide
Kisspeptin-10
Outcome
Not applicableKiss-1 mRNA level and lymph node involvement
Study
n: not reported in source
Outcome group
Tumor growth · Cancer
Source sentences
Summary
In human colorectal cancer tissues, the mRNA expression level of Kiss-1 had a negative correlation with Dukes staging, TNM staging, tumour size and lymph node involvement.
1 context sentence(design, population, supporting numbers)
Summary · context
The mRNA and protein expression of Kiss-1 and Kiss-1R, and their correlation to the clinical outcome in human colorectal cancer were investigated using real-time PCR and IHC respectively.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2526078509

Studies that did not test the peptide alone2

In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-10.

08LabNot a test of Kisspeptin-10 aloneIn vitro (cells)Mechanism
In colorectal cancer cells, knocking down Kiss-1 increased invasion.
In plain wordsIn cancer cells in the lab, switching off the Kiss-1 gene made colorectal cancer cells invade more.
Peptide
Kisspeptin-10
Outcome
IncreaseInvasion after Kiss-1 knockdown
Study
humann: not reported in source
Colorectal cancer cells
Outcome group
Tumor growth · Cancer
Source sentences
Summary
Knocking down Kiss-1 resulted in increased invasion
1 context sentence(design, population, supporting numbers)
Summary · context
Ribozyme transgenes were used to knockdown high expression of Kiss-1 and Kiss-1R in HT115 and HRT18 cells.
In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.
Show in the study text Report a problemFND-2526078504
09LabNot a test of Kisspeptin-10 aloneIn vitro (cells)Mechanism
In colorectal cancer cells, knocking down Kiss-1 increased migration.
In plain wordsIn cancer cells in the lab, switching off the Kiss-1 gene made colorectal cancer cells move more.
Peptide
Kisspeptin-10
Outcome
IncreaseMigration after Kiss-1 knockdown
Study
humann: not reported in source
Colorectal cancer cells
Outcome group
Tumor growth · Cancer
Source sentences
Summary
Knocking down Kiss-1 resulted in increased invasion and migration of colorectal cancer cells.
1 context sentence(design, population, supporting numbers)
Summary · context
Ribozyme transgenes were used to knockdown high expression of Kiss-1 and Kiss-1R in HT115 and HRT18 cells.
In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.
Show in the study text Report a problemFND-2526078505

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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