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Study · Animal study (in vivo) · mouse · 2014

7 results

Thymosin β4 administration enhances fracture healing in mice.

Brady RD, Grills BL, Schuijers JA, Ward AR, Tonkin BA, Walsh NC, McDonald SJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society8 Jul 2014Cited by 14 (Europe PMC)

PMID25042765 (opens in a new tab)DOI10.1002/jor.22686 (opens in a new tab)PepBankPUB-25042765
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice with fibular osteotomy, Tβ4-treated calluses had a 41% increase in peak force to failure compared with saline-treated controls.

    In plain wordsIn mice with a bone fracture, healed bone treated with Tβ4 was stronger than with saline.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreasePeak force to failure of fracture callus
    Study

    mousen: not reported in source

    bilateral fibular osteotomy
    Comparator
    Saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    6 mg/kg · intraperitonealAs given in this study (mouse).

    Measurements

    Peak force to failure increase
    41%
    Tβ4 vs saline

    Source sentences

    Summary
    Biomechanical analysis showed that Tβ4 -treated calluses had a 41% increase in peak force to failure (p < 0.01) and were approximately 25% stiffer (p < 0.05) than saline-treated controls
    1 context sentence
    Summary · context
    Mice received a bilateral fibular osteotomy and were given i.p. injections of either Tβ4 (6 mg/kg) or saline.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In mice with fibular osteotomy, Tβ4-treated calluses were approximately 25% stiffer than saline-treated controls.

    In plain wordsIn mice with a bone fracture, healed bone treated with Tβ4 was stiffer than with saline.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseStiffness of fracture callus
    Study

    mousen: not reported in source

    bilateral fibular osteotomy
    Comparator
    Saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    6 mg/kg · intraperitonealAs given in this study (mouse).

    Measurements

    Callus stiffness increase
    ≈ 25%
    Tβ4 vs saline

    Source sentences

    Summary
    Biomechanical analysis showed that Tβ4 -treated calluses had a 41% increase in peak force to failure (p < 0.01) and were approximately 25% stiffer (p < 0.05) than saline-treated controls
    1 context sentence
    Summary · context
    Mice received a bilateral fibular osteotomy and were given i.p. injections of either Tβ4 (6 mg/kg) or saline.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In mice with fibular osteotomy, the fractional volume of new mineralized tissue was 18% greater in Tβ4-treated calluses than in controls at 21 days.

    In plain wordsIn mice with a bone fracture, Tβ4 treatment gave more new mineralized bone tissue than saline.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseNew mineralized tissue fractional volume at 21 days
    Study

    mousen: not reported in source

    bilateral fibular osteotomy
    Comparator
    Saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    6 mg/kg · intraperitonealAs given in this study (mouse).

    Measurements

    New mineralized tissue fractional volume increase
    18%
    Tβ4 vs control

    Source sentences

    Summary
    µCT analysis at 21 days post-fracture showed that the fractional volume of new mineralized tissue and new highly mineralized tissue were respectively 18% and 26% greater in calluses from Tβ4 -treated mice compared to controls (p < 0.01; p < 0.05, respectively)
    1 context sentence
    Summary · context
    Mice received a bilateral fibular osteotomy and were given i.p. injections of either Tβ4 (6 mg/kg) or saline.
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In mice with fibular osteotomy, the fractional volume of new highly mineralized tissue was 26% greater in Tβ4-treated calluses than in controls at 21 days.

    In plain wordsIn mice with a bone fracture, Tβ4 treatment gave more new, highly mineralized bone tissue than saline.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseNew highly mineralized tissue fractional volume at 21 days
    Study

    mousen: not reported in source

    bilateral fibular osteotomy
    Comparator
    Saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    6 mg/kg · intraperitonealAs given in this study (mouse).

    Measurements

    New highly mineralized tissue fractional volume increase
    26%
    Tβ4 vs control

    Source sentences

    Summary
    µCT analysis at 21 days post-fracture showed that the fractional volume of new mineralized tissue and new highly mineralized tissue were respectively 18% and 26% greater in calluses from Tβ4 -treated mice compared to controls (p < 0.01; p < 0.05, respectively)
    1 context sentence
    Summary · context
    Mice received a bilateral fibular osteotomy and were given i.p. injections of either Tβ4 (6 mg/kg) or saline.
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    In mice with fibular osteotomy, Tβ4-treated calluses were almost 23% smaller than controls at 21 days.

    In plain wordsIn mice with a bone fracture, the healing callus was smaller with Tβ4 than with saline.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseCallus size at 21 days
    Study

    mousen: not reported in source

    bilateral fibular osteotomy
    Comparator
    Saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    6 mg/kg · intraperitonealAs given in this study (mouse).

    Measurements

    Callus size reduction
    ≈ 23%
    Tβ4 vs control

    Source sentences

    Summary
    Tβ4 -treated calluses were almost 23% smaller (p < 0.05)
    1 context sentence
    Summary · context
    Mice received a bilateral fibular osteotomy and were given i.p. injections of either Tβ4 (6 mg/kg) or saline.
  6. 06AnimalAnimal study (in vivo)Efficacy outcome

    In mice with fibular osteotomy, Tβ4-treated calluses had nearly 47% less old cortical bone than controls at 21 days.

    In plain wordsIn mice with a bone fracture, Tβ4-treated calluses held less old bone than saline-treated ones.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseOld cortical bone in callus at 21 days
    Study

    mousen: not reported in source

    bilateral fibular osteotomy
    Comparator
    Saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    6 mg/kg · intraperitonealAs given in this study (mouse).

    Measurements

    Old cortical bone reduction
    ≈ 47%
    Tβ4 vs control

    Source sentences

    Summary
    had nearly 47% less old cortical bone (p < 0.05)
    1 context sentence
    Summary · context
    Mice received a bilateral fibular osteotomy and were given i.p. injections of either Tβ4 (6 mg/kg) or saline.
  7. 07AnimalAnimal study (in vivo)Efficacy outcome

    In mice with fibular osteotomy, Tβ4-treated calluses had a 31% increase in the new trabecular bone area to total callus area fraction compared with controls at 21 days.

    In plain wordsIn mice with a bone fracture, Tβ4 treatment gave more new spongy bone in the callus than saline.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseNew trabecular bone area to total callus area fraction at 21 days
    Study

    mousen: not reported in source

    bilateral fibular osteotomy
    Comparator
    Saline
    Outcome group
    Bone and joint · Musculoskeletal
    Studied dose
    6 mg/kg · intraperitonealAs given in this study (mouse).

    Measurements

    New trabecular bone area fraction increase
    31%
    Tβ4 vs control

    Source sentences

    Summary
    had a 31% increase in new trabecular bone area/total callus area fraction compared with controls (p < 0.05)
    1 context sentence
    Summary · context
    Mice received a bilateral fibular osteotomy and were given i.p. injections of either Tβ4 (6 mg/kg) or saline.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

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