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Study · In vitro (cells) · rat · 2014

4 results

Humanin rescues cultured rat cortical neurons from NMDA-induced toxicity not by NMDA receptor.

Cui AL, Li JZ, Feng ZB, Ma GL, Gong L, Li CL, Zhang C, Li K

TheScientificWorldJournal19 May 2014Cited by 13 (Europe PMC)

PMID24959608 (opens in a new tab)PMCIDPMC4052483 (opens in a new tab)DOI10.1155/2014/341529 (opens in a new tab)PepBankPUB-24959608
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Summary

Excitatory neurotoxicity has been implicated in many pathological situations and there is no effective treatment available. Humanin is a 24-aa peptide cloned from the brain of patients with Alzheimer's disease (AD). In the present study, excitatory toxicity was induced by N-methyl-D-aspartate (NMDA) in primarily cultured rat cortical neurons. MTT assessment, lactate dehydrogenase (LDH) release, and calcein staining were employed to evaluate the protective activity of humanin on NMDA induced toxicity. The results suggested that NMDA (100 μmol/L, 2.5 hr) triggered neuronal morphological changes, lactate dehydrogenase (LDH) release (166% of the control), reduction of cell viability (about 50% of the control), and the decrease of living cell density (about 50% of the control). When pretreated with humanin, the toxicity was suppressed. The living cells' density of humanin treated group was similar to that of control. The cell viability was attenuated dose-dependently (IC50 = 0.132 nmol/L). The LDH release was also neutralized in a dose-dependent manner. In addition, the intracellular Ca(2+) overloading triggered by NMDA reverted quickly and humanin could not inhibit it. These findings indicate that humanin can rescue cortical neurons from NMDA-induced toxicity in rat but not through interfering with NMDA receptor directly.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In rat cortical neurons, humanin pretreatment attenuated the NMDA-induced loss of cell viability in a dose-dependent manner.

    In plain wordsIn rat brain cells in a dish, humanin pretreatment reduced the loss of cell viability caused by NMDA, with higher doses working better.

    Peptide
    Humanin
    Outcome
    IncreaseCell viability after NMDA toxicity
    Study

    ratn: not reported in source

    primary cultured cortical neurons
    Comparator
    NMDA without humanin
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Measurements

    IC50
    0.132 nmol/L
    cell viability, NMDA-induced toxicity

    Source sentences

    Summary
    The cell viability was attenuated dose-dependently (IC50 = 0.132 nmol/L).
    2 context sentences
    Summary · context
    The results suggested that NMDA (100 μmol/L, 2.5 hr) triggered neuronal morphological changes, lactate dehydrogenase (LDH) release (166% of the control), reduction of cell viability (about 50% of the control), and the decrease of living cell density (about 50% of the control).
    Summary · context
    In the present study, excitatory toxicity was induced by N-methyl-D-aspartate (NMDA) in primarily cultured rat cortical neurons.
  2. 02LabIn vitro (cells)Efficacy outcome

    In rat cortical neurons exposed to NMDA, the living cell density in the humanin-treated group was similar to that of control, while NMDA alone had reduced it to about 50% of control.

    In plain wordsIn rat brain cells in a dish, humanin-treated cells kept a living-cell density like untreated control cells, while NMDA alone cut it to about half.

    Peptide
    Humanin
    Outcome
    IncreaseDensity of living cells after NMDA toxicity
    Study

    ratn: not reported in source

    primary cultured cortical neurons
    Comparator
    NMDA without humanin
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The living cells' density of humanin treated group was similar to that of control.
    1 context sentence
    Summary · context
    The results suggested that NMDA (100 μmol/L, 2.5 hr) triggered neuronal morphological changes, lactate dehydrogenase (LDH) release (166% of the control), reduction of cell viability (about 50% of the control), and the decrease of living cell density (about 50% of the control).
  3. 03LabIn vitro (cells)Efficacy outcome

    In rat cortical neurons, humanin pretreatment neutralized NMDA-induced lactate dehydrogenase release in a dose-dependent manner.

    In plain wordsIn rat brain cells in a dish, humanin pretreatment reduced the cell-damage signal released after NMDA, with higher doses working better.

    Peptide
    Humanin
    Outcome
    DecreaseLDH release after NMDA toxicity
    Study

    ratn: not reported in source

    primary cultured cortical neurons
    Comparator
    NMDA without humanin
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The LDH release was also neutralized in a dose-dependent manner.
    2 context sentences
    Summary · context
    The results suggested that NMDA (100 μmol/L, 2.5 hr) triggered neuronal morphological changes, lactate dehydrogenase (LDH) release (166% of the control), reduction of cell viability (about 50% of the control), and the decrease of living cell density (about 50% of the control).
    Summary · context
    In the present study, excitatory toxicity was induced by N-methyl-D-aspartate (NMDA) in primarily cultured rat cortical neurons.
  4. 04LabIn vitro (cells)Mechanism

    In rat cortical neurons, humanin could not inhibit the intracellular Ca(2+) overloading triggered by NMDA.

    In plain wordsIn rat brain cells in a dish, humanin did not stop the calcium overload that NMDA triggered.

    Peptide
    Humanin
    Outcome
    No significant changeNMDA-induced intracellular Ca(2+) overloading
    Study

    ratn: not reported in source

    primary cultured cortical neurons
    Comparator
    NMDA without humanin
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In addition, the intracellular Ca(2+) overloading triggered by NMDA reverted quickly and humanin could not inhibit it.
    1 context sentence
    Summary · context
    In the present study, excitatory toxicity was induced by N-methyl-D-aspartate (NMDA) in primarily cultured rat cortical neurons.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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