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Study · Animal study (in vivo) · mouse · 2013

8 results

Decapeptyl ameliorates cyclophosphamide-induced reproductive toxicity in male Balb/C mice: histomorphometric, stereologic and hormonal evidences.

Niakani A, Farrokhi F, Hasanzadeh S

Iranian journal of reproductive medicine1 Oct 2013Cited by 6 (Europe PMC)

PMID24639699 (opens in a new tab)PMCIDPMC3941336 (opens in a new tab)PepBankPUB-24639699
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Summary

BackgroundGonadotropin-releasing hormone (GnRH) is a reproductive key hormone. The GnRH analogues are widely used in in vitro fertilization and treatment of sex hormone-depended cancers induced by the materials used in chemotherapeutic agents.ObjectiveThe aim of this study is to evaluate the effects of cyclophosphamide and decapeptyl (analogues of GnRH) on histomorphometry and stereology of testicular tissue as well as gonadotropic and gonadal hormones indices in mice.Materials and methodsFor this study, 24 adult male Balb/C strain mice were divided in four groups; first, cyclophosphamide (65 mg/kg/body weight (BW)), second, decapeptyl (0.05 mg/kg/BW), third, decapeptyl at first, and after 10 days of cyclophosphamide injection, and control group was received same volume of sterile saline. In order to evaluate the tissue changes in testes of the mice, sections were prepared and stained with Hematoxylin-Eosine, Periodic Acid Schief's (PAS) and Oil-Red-O staining techniques.ResultsThe cyclophosphamide causes histomorphologic changes in the testicular tissue; whereas such changes by decapeptyl were comparatively mild. The morphometric results revealed significant reduction in diameters of seminiferous tubules (p=0.02), and the stereological results confirmed significant differences in spermatogenesis (SI) as well as rate of tubal differentiation (TDI) indices between experimental and control groups (p=0.001). In addition, the morphometric findings proved that, there are significant decrease (p=0.001) in thicknesses of epithelia and stereologic result revealed reduction in number of cell layers in both decapeptyl and chemotherapy groups, but the decrements of these parameters were significant (p=0.02) in later group. In groups that had received cyclophosphamide, and decapeptyl alone, the LH and testosterone levels were decreased significantly (p=0.03), whereas in those that had received decapeptyl along with cyclophosphamide, the LH and FSH levels showed a decline but the level of testosterone increased.ConclusionThese results demonstrated that, analogue of GnRH i.e., decapeptyl protect morphologic, morphometric, and stereologic alterations of the testes tissue, as well as gonadotropic and gonadal hormonal changes preceding cyclophosphamide treatment in male mice. This article extracted from M.Sc. thesis. (Afsaneh Niakani).
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study8

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Safety outcome

    In male mice, testicular histomorphologic changes with decapeptyl were milder than those with cyclophosphamide.

    In plain wordsIn mice, decapeptyl produced milder testicular tissue changes than cyclophosphamide.

    Peptide
    Triptorelin (Trelstar, Decapeptyl)
    Outcome
    DecreaseTesticular histomorphologic changes
    Study

    mousen: not reported in source

    male Balb/C mice
    Comparator
    cyclophosphamide
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    0.05 mg/kg/BWAs given in this study (mouse).

    Source sentences

    Summary
    The cyclophosphamide causes histomorphologic changes in the testicular tissue; whereas such changes by decapeptyl were comparatively mild.
    1 context sentence
    Summary · context
    For this study, 24 adult male Balb/C strain mice were divided in four groups; first, cyclophosphamide (65 mg/kg/body weight (BW)), second, decapeptyl (0.05 mg/kg/BW), third, decapeptyl at first, and after 10 days of cyclophosphamide injection, and control group was received same volume of sterile saline.
  2. 02AnimalAnimal study (in vivo)Safety outcome

    In male mice, testicular epithelium thickness decreased with decapeptyl versus control.

    In plain wordsIn mice, decapeptyl reduced testicular epithelium thickness compared with saline control.

    Peptide
    Triptorelin (Trelstar, Decapeptyl)
    Outcome
    DecreaseTesticular epithelium thickness
    Study

    mousen: not reported in source

    male Balb/C mice
    Comparator
    saline control
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    0.05 mg/kg/BWAs given in this study (mouse).

    Source sentences

    Summary
    In addition, the morphometric findings proved that, there are significant decrease (p=0.001) in thicknesses of epithelia and stereologic result revealed reduction in number of cell layers in both decapeptyl and chemotherapy groups, but the decrements of these parameters were significant (p=0.02) in later group.
    1 context sentence
    Summary · context
    For this study, 24 adult male Balb/C strain mice were divided in four groups; first, cyclophosphamide (65 mg/kg/body weight (BW)), second, decapeptyl (0.05 mg/kg/BW), third, decapeptyl at first, and after 10 days of cyclophosphamide injection, and control group was received same volume of sterile saline.
  3. 03AnimalAnimal study (in vivo)Safety outcome

    In male mice, decapeptyl reduced testicular cell layers, but significance was reported only for chemotherapy.

    In plain wordsIn mice, decapeptyl reduced testicular cell layers compared with saline control, but the abstract did not report a significant difference.

    Peptide
    Triptorelin (Trelstar, Decapeptyl)
    Outcome
    DecreaseTesticular cell layers
    Study

    mousen: not reported in source

    male Balb/C mice
    Comparator
    saline control
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    0.05 mg/kg/BWAs given in this study (mouse).

    Source sentences

    Summary
    In addition, the morphometric findings proved that, there are significant decrease (p=0.001) in thicknesses of epithelia and stereologic result revealed reduction in number of cell layers in both decapeptyl and chemotherapy groups, but the decrements of these parameters were significant (p=0.02) in later group.
    1 context sentence
    Summary · context
    For this study, 24 adult male Balb/C strain mice were divided in four groups; first, cyclophosphamide (65 mg/kg/body weight (BW)), second, decapeptyl (0.05 mg/kg/BW), third, decapeptyl at first, and after 10 days of cyclophosphamide injection, and control group was received same volume of sterile saline.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In male mice, decapeptyl alone significantly decreased LH versus control.

    In plain wordsIn mice, decapeptyl alone lowered LH compared with saline control.

    Peptide
    Triptorelin (Trelstar, Decapeptyl)
    Outcome
    DecreaseLH with decapeptyl alone
    Study

    mousen: not reported in source

    male Balb/C mice
    Comparator
    saline control
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    0.05 mg/kg/BWAs given in this study (mouse).

    Source sentences

    Summary
    In groups that had received cyclophosphamide, and decapeptyl alone, the LH and testosterone levels were decreased significantly (p=0.03), whereas in those that had received decapeptyl along with cyclophosphamide, the LH and FSH levels showed a decline but the level of testosterone increased.
    1 context sentence
    Summary · context
    For this study, 24 adult male Balb/C strain mice were divided in four groups; first, cyclophosphamide (65 mg/kg/body weight (BW)), second, decapeptyl (0.05 mg/kg/BW), third, decapeptyl at first, and after 10 days of cyclophosphamide injection, and control group was received same volume of sterile saline.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In male mice, decapeptyl alone significantly decreased testosterone versus control.

    In plain wordsIn mice, decapeptyl alone lowered testosterone compared with saline control.

    Peptide
    Triptorelin (Trelstar, Decapeptyl)
    Outcome
    DecreaseTestosterone with decapeptyl alone
    Study

    mousen: not reported in source

    male Balb/C mice
    Comparator
    saline control
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    0.05 mg/kg/BWAs given in this study (mouse).

    Source sentences

    Summary
    In groups that had received cyclophosphamide, and decapeptyl alone, the LH and testosterone levels were decreased significantly (p=0.03), whereas in those that had received decapeptyl along with cyclophosphamide, the LH and FSH levels showed a decline but the level of testosterone increased.
    1 context sentence
    Summary · context
    For this study, 24 adult male Balb/C strain mice were divided in four groups; first, cyclophosphamide (65 mg/kg/body weight (BW)), second, decapeptyl (0.05 mg/kg/BW), third, decapeptyl at first, and after 10 days of cyclophosphamide injection, and control group was received same volume of sterile saline.

Studies that did not test the peptide alone3

In this study, Triptorelin (Trelstar, Decapeptyl) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Triptorelin (Trelstar, Decapeptyl).

  1. 06AnimalNot a test of Triptorelin (Trelstar, Decapeptyl) aloneAnimal study (in vivo)Mechanism

    In male mice receiving decapeptyl and cyclophosphamide, LH declined; the abstract did not specify the comparator.

    In plain wordsIn mice receiving decapeptyl and cyclophosphamide, LH declined; the comparison was not specified.

    Peptide
    Triptorelin (Trelstar, Decapeptyl)
    Outcome
    DecreaseLH with decapeptyl and cyclophosphamide
    Study

    mousen: not reported in source

    male Balb/C mice
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    In groups that had received cyclophosphamide, and decapeptyl alone, the LH and testosterone levels were decreased significantly (p=0.03), whereas in those that had received decapeptyl along with cyclophosphamide, the LH and FSH levels showed a decline but the level of testosterone increased.
    1 context sentence
    Summary · context
    For this study, 24 adult male Balb/C strain mice were divided in four groups; first, cyclophosphamide (65 mg/kg/body weight (BW)), second, decapeptyl (0.05 mg/kg/BW), third, decapeptyl at first, and after 10 days of cyclophosphamide injection, and control group was received same volume of sterile saline.

    In this study, Triptorelin (Trelstar, Decapeptyl) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Triptorelin (Trelstar, Decapeptyl).

  2. 07AnimalNot a test of Triptorelin (Trelstar, Decapeptyl) aloneAnimal study (in vivo)Mechanism

    In male mice receiving decapeptyl and cyclophosphamide, FSH declined; the abstract did not specify the comparator.

    In plain wordsIn mice receiving decapeptyl and cyclophosphamide, FSH declined; the comparison was not specified.

    Peptide
    Triptorelin (Trelstar, Decapeptyl)
    Outcome
    DecreaseFSH with decapeptyl and cyclophosphamide
    Study

    mousen: not reported in source

    male Balb/C mice
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    In groups that had received cyclophosphamide, and decapeptyl alone, the LH and testosterone levels were decreased significantly (p=0.03), whereas in those that had received decapeptyl along with cyclophosphamide, the LH and FSH levels showed a decline but the level of testosterone increased.
    1 context sentence
    Summary · context
    For this study, 24 adult male Balb/C strain mice were divided in four groups; first, cyclophosphamide (65 mg/kg/body weight (BW)), second, decapeptyl (0.05 mg/kg/BW), third, decapeptyl at first, and after 10 days of cyclophosphamide injection, and control group was received same volume of sterile saline.

    In this study, Triptorelin (Trelstar, Decapeptyl) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Triptorelin (Trelstar, Decapeptyl).

  3. 08AnimalNot a test of Triptorelin (Trelstar, Decapeptyl) aloneAnimal study (in vivo)Mechanism

    In male mice receiving decapeptyl and cyclophosphamide, testosterone increased; the abstract did not specify the comparator.

    In plain wordsIn mice receiving decapeptyl and cyclophosphamide, testosterone increased; the comparison was not specified.

    Peptide
    Triptorelin (Trelstar, Decapeptyl)
    Outcome
    IncreaseTestosterone with decapeptyl and cyclophosphamide
    Study

    mousen: not reported in source

    male Balb/C mice
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    In groups that had received cyclophosphamide, and decapeptyl alone, the LH and testosterone levels were decreased significantly (p=0.03), whereas in those that had received decapeptyl along with cyclophosphamide, the LH and FSH levels showed a decline but the level of testosterone increased.
    1 context sentence
    Summary · context
    For this study, 24 adult male Balb/C strain mice were divided in four groups; first, cyclophosphamide (65 mg/kg/body weight (BW)), second, decapeptyl (0.05 mg/kg/BW), third, decapeptyl at first, and after 10 days of cyclophosphamide injection, and control group was received same volume of sterile saline.

    In this study, Triptorelin (Trelstar, Decapeptyl) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Triptorelin (Trelstar, Decapeptyl).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    22 Oct 2026, 02:26 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 18:59 UTC

    First added · source update from Europe PMC

Subjects

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