Thymosin beta-4 knockdown in IEC-6 normal intestinal epithelial cells induces DNA re-replication via downregulating Emi1.

Chao TC, Chen KJ, Tang MC, Chan LC, Chen PM, Tzeng CH, Su Y

Journal of cellular physiology1 Nov 2014Cited by 8 (Europe PMC)

PMID24615569DOI10.1002/jcp.24609PepBankPUB-24615569

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone9

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome
In rat IEC-6 intestinal epithelial cells, shRNA knockdown of Tβ4 significantly suppressed cell growth.
In plain wordsIn rat intestinal cells, lowering the cell's own Tβ4 slowed cell growth.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseIEC-6 cell growth after Tβ4 knockdown
Study
ratn: not reported in source
IEC-6 normal rat small intestinal cells with shRNA knockdown of Tβ4
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
inhibiting Tβ4 expression significantly suppressed their growth and induced apoptosis in some cells.
1 context sentence(design, population, supporting numbers)
Summary · context
we examined the consequences of shRNA-mediated knockdown of Tβ4 in IEC-6 normal rat small intestinal cells
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2461556901
02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome
In rat IEC-6 intestinal epithelial cells, shRNA knockdown of Tβ4 induced apoptosis in some cells.
In plain wordsIn rat intestinal cells, lowering the cell's own Tβ4 caused some cells to die by apoptosis.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseApoptosis after Tβ4 knockdown
Study
ratn: not reported in source
IEC-6 normal rat small intestinal cells with shRNA knockdown of Tβ4
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
induced apoptosis in some cells.
1 context sentence(design, population, supporting numbers)
Summary · context
we examined the consequences of shRNA-mediated knockdown of Tβ4 in IEC-6 normal rat small intestinal cells
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2461556902
03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In IEC-6 cells with Tβ4 knockdown, flow cytometry showed a marked decrease of the G0/G1 population.
In plain wordsIn rat intestinal cells with less Tβ4, fewer cells stayed in the resting G0/G1 phase.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseG0/G1 cell population
Study
ratn: not reported in source
IEC-6 normal rat small intestinal cells with shRNA knockdown of Tβ4
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
Flow cytometric analysis further revealed a marked decrease of G0/G1 population but a drastic increase of polyploid ones in these cells.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we assessed whether Tβ4 alteration influences normal intestinal epithelial cells because Tβ4 is deemed a novel target for treating colorectal cancer (CRC).
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2461556903
04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In IEC-6 cells with Tβ4 knockdown, flow cytometry showed a drastic increase of polyploid cells.
In plain wordsIn rat intestinal cells with less Tβ4, many more cells had extra sets of chromosomes.
Peptide
Thymosin beta-4 (human)
Outcome
IncreasePolyploid cell population
Study
ratn: not reported in source
IEC-6 normal rat small intestinal cells with shRNA knockdown of Tβ4
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
Flow cytometric analysis further revealed a marked decrease of G0/G1 population but a drastic increase of polyploid ones in these cells.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we assessed whether Tβ4 alteration influences normal intestinal epithelial cells because Tβ4 is deemed a novel target for treating colorectal cancer (CRC).
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2461556904
05LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In IEC-6 cells with Tβ4 knockdown, de novo DNA synthesis was greatly increased, consistent with DNA re-replication.
In plain wordsIn rat intestinal cells with less Tβ4, new DNA synthesis rose sharply.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseDe novo DNA synthesis
Study
ratn: not reported in source
IEC-6 normal rat small intestinal cells with shRNA knockdown of Tβ4
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
not only the de novo DNA synthesis was greatly increased but also the expression levels of Cdc6 (a replication-licensing factor), cyclin A, and phosphorylated-checkpoint kinase 1 were all dramatically elevated.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we assessed whether Tβ4 alteration influences normal intestinal epithelial cells because Tβ4 is deemed a novel target for treating colorectal cancer (CRC).
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2461556905
06LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In IEC-6 cells with Tβ4 knockdown, expression of the replication-licensing factor Cdc6 was dramatically elevated.
In plain wordsIn rat intestinal cells with less Tβ4, the DNA replication factor Cdc6 rose sharply.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseCdc6 expression
Study
ratn: not reported in source
IEC-6 normal rat small intestinal cells with shRNA knockdown of Tβ4
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
the expression levels of Cdc6 (a replication-licensing factor), cyclin A, and phosphorylated-checkpoint kinase 1 were all dramatically elevated.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we assessed whether Tβ4 alteration influences normal intestinal epithelial cells because Tβ4 is deemed a novel target for treating colorectal cancer (CRC).
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2461556906
07LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In IEC-6 cells with Tβ4 knockdown, cyclin A expression was dramatically elevated.
In plain wordsIn rat intestinal cells with less Tβ4, cyclin A rose sharply.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseCyclin A expression
Study
ratn: not reported in source
IEC-6 normal rat small intestinal cells with shRNA knockdown of Tβ4
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
the expression levels of Cdc6 (a replication-licensing factor), cyclin A, and phosphorylated-checkpoint kinase 1 were all dramatically elevated.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we assessed whether Tβ4 alteration influences normal intestinal epithelial cells because Tβ4 is deemed a novel target for treating colorectal cancer (CRC).
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2461556907
08LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In IEC-6 cells with Tβ4 knockdown, phosphorylated checkpoint kinase 1 was dramatically elevated.
In plain wordsIn rat intestinal cells with less Tβ4, phosphorylated checkpoint kinase 1 rose sharply.
Peptide
Thymosin beta-4 (human)
Outcome
IncreasePhosphorylated checkpoint kinase 1 expression
Study
ratn: not reported in source
IEC-6 normal rat small intestinal cells with shRNA knockdown of Tβ4
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
the expression levels of Cdc6 (a replication-licensing factor), cyclin A, and phosphorylated-checkpoint kinase 1 were all dramatically elevated.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we assessed whether Tβ4 alteration influences normal intestinal epithelial cells because Tβ4 is deemed a novel target for treating colorectal cancer (CRC).
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2461556908
09LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In IEC-6 cells with Tβ4 knockdown, RNA and protein levels of Emi1 were markedly reduced.
In plain wordsIn rat intestinal cells with less Tβ4, Emi1 levels fell markedly.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseEmi1 RNA and protein levels
Study
ratn: not reported in source
IEC-6 normal rat small intestinal cells with shRNA knockdown of Tβ4
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
marked reductions in both RNA and protein levels of Emi1 (early mitotic inhibitor 1) were also detected in Tβ4 -downregulated IEC-6 cells
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we assessed whether Tβ4 alteration influences normal intestinal epithelial cells because Tβ4 is deemed a novel target for treating colorectal cancer (CRC).
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2461556909

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

Subjects

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