Genome expression analysis by suppression subtractive hybridization identified overexpression of Humanin, a target gene in gastric cancer chemoresistance.

Mottaghi-Dastjerdi N, Soltany-Rezaee-Rad M, Sepehrizadeh Z, Roshandel G, Ebrahimifard F, Setayesh N

Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences8 Jan 2014Cited by 28 (Europe PMC)

PMID24401285PMCIDPMC3896685DOI10.1186/2008-2231-22-14PepBankPUB-24401285

Summary

BackgroundIn cancer cells, apoptosis is an important mechanism that influences the outcome of chemotherapy and the development of chemoresistance. To find the genes involved in chemoresistance and the development of gastric cancer, we used the suppression subtractive hybridization method to identify the genes that are overexpressed in gastric cancer tissues compared to normal gastric tissues.ResultsIn the suppression subtractive hybridization library we constructed, the most highly overexpressed genes were humanin isoforms. Humanin is a recently identified endogenous peptide that has anti-apoptotic activity and has been selected for further study due to its potential role in the chemoresistance of gastric cancer. Upregulation of humanin isoforms was also observed in clinical samples by using quantitative real-time PCR. Among the studied isoforms, humanin isoform 3, with an expression level of 4.166 ± 1.44 fold, was the most overexpressed isoform in GC.ConclusionsThe overexpression of humanin in gastric cancer suggests a role for chemoresistance and provides new insight into the biology of gastric cancer. We propose that humanin isoforms are novel targets for combating chemoresistance in gastric cancer.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

No result from this study comes from a test of the peptide.

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01HumanEndogenous levelObservational studyOther
In gastric cancer tissue compared with normal gastric tissue, humanin isoforms were the most highly overexpressed genes in a suppression subtractive hybridization library.
In plain wordsIn gastric cancer tissue, the body's own humanin genes were among the most switched-on genes compared with normal stomach tissue.
Peptide
Humanin
Outcome
IncreaseHumanin isoform overexpression in gastric cancer library
Study
n: not reported in source
Comparator
Normal gastric tissue
Outcome group
Cancer biomarkers and prognosis · Cancer
Source sentences
Summary
In the suppression subtractive hybridization library we constructed, the most highly overexpressed genes were humanin isoforms.
1 context sentence(design, population, supporting numbers)
Summary · context
To find the genes involved in chemoresistance and the development of gastric cancer, we used the suppression subtractive hybridization method to identify the genes that are overexpressed in gastric cancer tissues compared to normal gastric tissues.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2440128501
02HumanEndogenous levelObservational studyOther
Quantitative real-time PCR of clinical samples showed upregulation of humanin isoforms in gastric cancer.
In plain wordsIn clinical stomach cancer samples, natural humanin levels were higher, linked to the cancer.
Peptide
Humanin
Outcome
IncreaseHumanin isoform upregulation in clinical samples by qPCR
Study
n: not reported in source
Comparator
Normal gastric tissue
Outcome group
Cancer biomarkers and prognosis · Cancer
Source sentences
Summary
Upregulation of humanin isoforms was also observed in clinical samples by using quantitative real-time PCR.
1 context sentence(design, population, supporting numbers)
Summary · context
To find the genes involved in chemoresistance and the development of gastric cancer, we used the suppression subtractive hybridization method to identify the genes that are overexpressed in gastric cancer tissues compared to normal gastric tissues.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2440128502
03HumanEndogenous levelObservational studyOther
Humanin isoform 3 was the most overexpressed isoform in gastric cancer, at 4.166 ± 1.44 fold.
In plain wordsIn stomach cancer, natural humanin isoform 3 was the most increased form of the peptide.
Peptide
Humanin
Outcome
IncreaseHumanin isoform 3 expression fold in gastric cancer
Study
n: not reported in source
Comparator
Normal gastric tissue
Outcome group
Cancer biomarkers and prognosis · Cancer
Measurements
Overexpression
4.166 fold
humanin isoform 3 in gastric cancer
Source sentences
Summary
Among the studied isoforms, humanin isoform 3, with an expression level of 4.166 ± 1.44 fold, was the most overexpressed isoform in GC.
1 context sentence(design, population, supporting numbers)
Summary · context
To find the genes involved in chemoresistance and the development of gastric cancer, we used the suppression subtractive hybridization method to identify the genes that are overexpressed in gastric cancer tissues compared to normal gastric tissues.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-2440128503

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Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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