Skip to content

Study · In vitro (cell-free) · 2014

10 results

Thymosin beta4 inhibits ADF/cofilin stimulated F-actin cycling and hela cell migration: reversal by active Arp2/3 complex.

Al Haj A, Mazur AJ, Buchmeier S, App C, Theiss C, Silvan U, Schoenenberger CA, Jockusch BM, Hannappel E, Weeds AG, Mannherz HG

Cytoskeleton (Hoboken, N.J.)31 Dec 2013Cited by 10 (Europe PMC)

PMID24382810 (opens in a new tab)DOI10.1002/cm.21128 (opens in a new tab)PepBankPUB-24382810
Publisher pagePubMedAsk about this study
Jump to 10 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cell-free)Activity measurement

    Tβ4 decreased F-actin treadmilling, whereas ADF/cofilin stimulated it.

    In plain wordsIn lab tests, thymosin beta‑4 slowed actin treadmilling, while cofilin sped it up.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseF-actin treadmilling with Tβ4
    Study

    n: not reported in source

    purified F-actin, ADF/cofilin
    Outcome group
    Receptor activity and mechanism · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Actin depolymerising factor (ADF)/cofilin stimulated treadmilling, while Tβ4 decreased treadmilling, presumably by sequestering monomers.
  2. 02LabIn vitro (cell-free)Activity measurement

    Tβ4 added together with ADF/cofilin inhibited treadmilling relative to cofilin alone.

    In plain wordsIn lab tests, thymosin beta‑4 added to cofilin slowed actin treadmilling compared with cofilin alone.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseTreadmilling with Tβ4 plus ADF/cofilin versus cofilin alone
    Study

    n: not reported in source

    purified F-actin, ADF/cofilin
    Comparator
    ADF/cofilin alone
    Outcome group
    Receptor activity and mechanism · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tβ4 added together with ADF/cofilin also inhibited the treadmilling, relative to cofilin alone

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 03LabEndogenous levelIn vitro (cells)Mechanism

    Immunofluorescence showed colocalisation of Tβ4 with cofilin-1 and Arp2/3 complex in lamellipodia.

    In plain wordsIn cells, the body's own thymosin beta‑4 was found in the same cell-edge areas as cofilin-1 and Arp2/3 complex.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableColocalisation of Tβ4 with cofilin-1 and Arp2/3 complex in lamellipodia
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Whole body

    Source sentences

    Summary
    immunofluorescence showed colocalisation of thymosin beta4 (Tβ4) with cofilin-1 and Arp2/3 complex in lamellipodia

    This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.

Studies that did not test the peptide alone7

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome

    Increased intracellular Tβ4 in transfected HeLa cells reduced actin filament bundles.

    In plain wordsIn cells, raising thymosin beta‑4 inside HeLa cells reduced actin filament bundles.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseActin filament bundles after Tβ4 increase
    Study

    humann: not reported in source

    HeLa cells
    Outcome group
    Receptor activity and mechanism · Whole body

    Source sentences

    Summary
    Transfection experiments to explore the effects of changing the intracellular concentration of Tβ4 in HeLa cells showed that an increase in Tβ4 resulted in reduced actin filaments bundles and narrower lamellipodia, and a conspicuous decrease of cell migration as seen by two different assays.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  2. 05LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome

    Increased intracellular Tβ4 in transfected HeLa cells produced narrower lamellipodia.

    In plain wordsIn cells, raising thymosin beta‑4 inside HeLa cells made the cell front edges narrower.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseLamellipodia width after Tβ4 increase
    Study

    humann: not reported in source

    HeLa cells
    Outcome group
    Receptor activity and mechanism · Whole body

    Source sentences

    Summary
    Transfection experiments to explore the effects of changing the intracellular concentration of Tβ4 in HeLa cells showed that an increase in Tβ4 resulted in reduced actin filaments bundles and narrower lamellipodia, and a conspicuous decrease of cell migration as seen by two different assays.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  3. 06LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome

    Increased intracellular Tβ4 in transfected HeLa cells decreased cell migration in two assays.

    In plain wordsIn cells, raising thymosin beta‑4 inside HeLa cells reduced cell movement.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseHeLa cell migration after Tβ4 increase
    Study

    humann: not reported in source

    HeLa cells
    Outcome group
    Cancer cell invasion and metastasis · Whole body

    Source sentences

    Summary
    Transfection experiments to explore the effects of changing the intracellular concentration of Tβ4 in HeLa cells showed that an increase in Tβ4 resulted in reduced actin filaments bundles and narrower lamellipodia, and a conspicuous decrease of cell migration as seen by two different assays.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  4. 07LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome

    Tβ4 knockdown by siRNA in HeLa cells produced prominent actin filament networks in the lamellipodia and leading lamella.

    In plain wordsIn cells, switching off thymosin beta‑4 in HeLa cells produced more prominent actin networks at the cell front.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseActin filament networks after Tβ4 knockdown
    Study

    humann: not reported in source

    HeLa cells
    Outcome group
    Receptor activity and mechanism · Whole body

    Source sentences

    Summary
    In contrast, cells transfected with a vector leading to Tβ4 knockdown by small interfering RNA (siRNA) displayed prominent actin filament networks within the lamellipodia and the leading lamella and enhanced migration.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  5. 08LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome

    Tβ4 knockdown by siRNA in HeLa cells enhanced migration.

    In plain wordsIn cells, switching off thymosin beta‑4 in HeLa cells increased cell movement.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseHeLa cell migration after Tβ4 knockdown
    Study

    humann: not reported in source

    HeLa cells
    Outcome group
    Cancer cell invasion and metastasis · Whole body

    Source sentences

    Summary
    In contrast, cells transfected with a vector leading to Tβ4 knockdown by small interfering RNA (siRNA) displayed prominent actin filament networks within the lamellipodia and the leading lamella and enhanced migration.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  6. 09LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    The rate and extent of depolymerization were markedly enhanced in the presence of both Tβ4 and ADF/cofilin.

    In plain wordsIn lab tests, actin broke down faster and further with both thymosin beta‑4 and cofilin present.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseRate and extent of depolymerization with Tβ4 plus ADF/cofilin
    Study

    n: not reported in source

    purified F-actin, ADF/cofilin
    Outcome group
    Receptor activity and mechanism · Whole body

    Source sentences

    Summary
    but both the rate and extent of depolymerization were markedly enhanced in the presence of both these proteins.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  7. 10LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    Arp2/3 complex reversed the sequestering activity of Tβ4 when equimolar to actin, but not in the additional presence of cofilin-1 or ADF.

    In plain wordsIn lab tests, Arp2/3 complex reversed thymosin beta‑4's actin-binding effect, but not when cofilin-1 or ADF was also present.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    MixedReversal of Tβ4 sequestering activity by Arp2/3 complex
    Study

    n: not reported in source

    purified actin, Arp2/3 complex
    Outcome group
    Receptor activity and mechanism · Whole body

    Source sentences

    Summary
    Arp2/3 complex reversed the sequestering activity of Tβ4 when equimolar to actin, but not in the additional presence of cofilin-1 or ADF.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.