Kisspeptin-10 modulates the proliferation and differentiation of the rhesus monkey derived stem cell line: R366.4.

Huma T, Wang Z, Rizak J, Ahmad F, Shahab M, Ma Y, Yang S, Hu X

TheScientificWorldJournal28 Nov 2013Cited by 12 (Europe PMC)

PMID24381507PMCIDPMC3863535DOI10.1155/2013/135470PepBankPUB-24381507

Summary

The rhesus monkey embryonic stem cell line R366.4 has been identified to differentiate into a number of cell types. However, it has not been well characterized for its response to drugs affecting reproductive endocrinology. Kisspeptins (KPs) are ligands for the GPR-54, which is known to modulate reproductive function. The current study was designed to determine the effect of the KP-10 peptide on R366.4 cells and to investigate the role of KP-GPR54 in the cell proliferation process. Four different doses (0.1, 1, 10, and 100 nM) of KP-10 and control were selected to evaluate cell growth parameters and cellular morphological changes over a 72 hr period. The cells were treated with kisspeptin-10 during the early rosette stage. Proliferation rates, analyzed by flow cytometry and cell count methods, were found to be decreased after treatment. Moreover, the number of rosettes was found to decrease following KP-10 treatments. Morphological changes consisting of neuronal projections were also witnessed. This suggested that KP-10 had an antiproliferative effect on R366.4 cells leading to a differentiation state and morphological changes consistent with neuronal stem cell development. The R366.4 stem cell line differentiates based on kisspeptin signaling and may be used to investigate reproductive cell endocrinology in vitro.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In rhesus monkey R366.4 stem cells, kisspeptin‑10 decreased proliferation rates on flow cytometry and cell count.
In plain wordsIn lab tests on monkey stem cells, kisspeptin‑10 slowed cell growth.
Peptide
Kisspeptin-10
Outcome
DecreaseCell proliferation rate
Study
rhesus monkeyn: not reported in source
R366.4 embryonic stem cell line
Comparator
Control
Outcome group
Cell proliferation and differentiation · Reproductive and sexual health
Studied dose
0.1, 1, 10 and 100 nM · 72 hrAs given in this study (rhesus monkey).
Source sentences
Summary
Proliferation rates, analyzed by flow cytometry and cell count methods, were found to be decreased after treatment.
1 context sentence(design, population, supporting numbers)
Summary · context
Four different doses (0.1, 1, 10, and 100 nM) of KP-10 and control were selected to evaluate cell growth parameters and cellular morphological changes over a 72 hr period.
Show in the study text Report a problemFND-2438150701
02LabIn vitro (cells)Efficacy outcome
In rhesus monkey R366.4 stem cells, kisspeptin‑10 treatment decreased the number of rosettes.
In plain wordsIn lab tests on monkey stem cells, kisspeptin‑10 reduced the number of rosettes.
Peptide
Kisspeptin-10
Outcome
DecreaseNumber of rosettes
Study
rhesus monkeyn: not reported in source
R366.4 embryonic stem cell line
Comparator
Control
Outcome group
Cell proliferation and differentiation · Reproductive and sexual health
Studied dose
0.1, 1, 10 and 100 nM · 72 hrAs given in this study (rhesus monkey).
Source sentences
Summary
Moreover, the number of rosettes was found to decrease following KP-10 treatments.
1 context sentence(design, population, supporting numbers)
Summary · context
Four different doses (0.1, 1, 10, and 100 nM) of KP-10 and control were selected to evaluate cell growth parameters and cellular morphological changes over a 72 hr period.
Show in the study text Report a problemFND-2438150702
03LabIn vitro (cells)Efficacy outcome
In rhesus monkey R366.4 stem cells, kisspeptin‑10 treatment was followed by morphological changes with neuronal projections.
In plain wordsIn lab tests on monkey stem cells, kisspeptin‑10 treatment was followed by nerve-like projections.
Peptide
Kisspeptin-10
Outcome
IncreaseMorphological change (neuronal projections)
Study
rhesus monkeyn: not reported in source
R366.4 embryonic stem cell line
Comparator
Control
Outcome group
Cell proliferation and differentiation · Reproductive and sexual health
Studied dose
0.1, 1, 10 and 100 nM · 72 hrAs given in this study (rhesus monkey).
Source sentences
Summary
Morphological changes consisting of neuronal projections were also witnessed.
1 context sentence(design, population, supporting numbers)
Summary · context
Four different doses (0.1, 1, 10, and 100 nM) of KP-10 and control were selected to evaluate cell growth parameters and cellular morphological changes over a 72 hr period.
Show in the study text Report a problemFND-2438150703

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Version history

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Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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