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Study · Animal study (in vivo) · mouse · 2013

9 results

Effect of the GLP-1 analog exendin-4 and oxaliplatin on intrahepatic cholangiocarcinoma cell line and mouse model.

Chen BD, Zhao WC, Jia QA, Zhou WY, Bu Y, Wang ZZ, Wang F, Wu WJ, Wang Q

International journal of molecular sciences13 Dec 2013Cited by 20 (Europe PMC)

PMID24351817 (opens in a new tab)PMCIDPMC3876111 (opens in a new tab)DOI10.3390/ijms141224293 (opens in a new tab)PepBankPUB-24351817
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Summary

The influence of Glucagon-like peptide-1 (GLP-1) and Exendin-4 on development of intrahepatic cholangiocarcinoma (ICC) is evaluated in the study. In vitro tests, including acute toxicity test, cell colony formation assays, cells proliferation and apoptosis, transwell assay, were performed. An ICC in situ tumor animal model was established. Then, animals were randomly divided into four groups (n = 6): control, Exendin-4 treatment, oxaliplatin treatment and Exendin-4-oxaliplatin treatment. Animals in the Exendin-4 treatment and Exendin-4-oxaliplatin treatment groups received a subcutaneous injection of Exendin-4 (100 μg/kg/day) for 1 week, and then received oxaliplatin (10 mg/kg/week) by tail vein injection. Animals in the control group received PBS. Immunohistochemistry tests were used for PCNA, Ki67, Caspase 3 expression in tumor tissue. Results show that that, after incubation of human cholangiocarcinoma cell lines, HuCCTI and GLP-1, or HuCCTI and Exendin-4, colony formation number was sharply decreased. However, GLP-1, HuCCTI or Exendin-4 did not affect the colony of normal cells. Combination treatment with oxaliplatin and Exendin-4 can significantly inhibit tumor cells' proliferation and promote apoptosis. The combined effect is stronger than that of oxaliplatin or Exendin-4. Combination treatment with oxaliplatin and Exendin4 can significantly decrease Ki67 and PCNA proteins' expression in subcutaneous tumors of nude mice. The inhibitory effect of Combination treatment with oxaliplatin and Exendin4 is clearly stronger than that of oxaliplatin. In addition, Combination treatment with oxaliplatin and Exendin4 can significantly increase Caspase3 protein positive expression. In short, these results show that combination treatment with oxaliplatin and Exendin4 can inhibit tumor cells' proliferation, and promote apoptosis.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study9

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Mechanism

    In nude mouse tumors, oxaliplatin plus exendin‑4 decreased Ki67 expression more than oxaliplatin alone.

    In plain wordsIn mice, oxaliplatin plus exendin‑4 reduced tumor Ki67 expression more than oxaliplatin alone.

    Peptide
    Exenatide (exendin-4)
    Outcome
    DecreaseKi67 expression
    Study

    mousen: not reported in source

    Nude mice with subcutaneous tumors
    Comparator
    Oxaliplatin alone
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Combination treatment with oxaliplatin and Exendin4 can significantly decrease Ki67 and PCNA proteins' expression in subcutaneous tumors of nude mice. The inhibitory effect of Combination treatment with oxaliplatin and Exendin4 is clearly stronger than that of oxaliplatin.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In nude mouse tumors, oxaliplatin plus exendin‑4 decreased PCNA expression more than oxaliplatin alone.

    In plain wordsIn mice, oxaliplatin plus exendin‑4 reduced tumor PCNA expression more than oxaliplatin alone.

    Peptide
    Exenatide (exendin-4)
    Outcome
    DecreasePCNA expression
    Study

    mousen: not reported in source

    Nude mice with subcutaneous tumors
    Comparator
    Oxaliplatin alone
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Combination treatment with oxaliplatin and Exendin4 can significantly decrease Ki67 and PCNA proteins' expression in subcutaneous tumors of nude mice. The inhibitory effect of Combination treatment with oxaliplatin and Exendin4 is clearly stronger than that of oxaliplatin.
  3. 03LabIn vitro (cells)Efficacy outcome

    In HuCCTI cholangiocarcinoma cells, exendin‑4 decreased colony formation.

    In plain wordsIn cells, exendin‑4 reduced cholangiocarcinoma colony formation.

    Peptide
    Exenatide (exendin-4)
    Outcome
    DecreaseColony formation
    Study

    humann: not reported in source

    HuCCTI cholangiocarcinoma cells
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Results show that that, after incubation of human cholangiocarcinoma cell lines, HuCCTI and GLP-1, or HuCCTI and Exendin-4, colony formation number was sharply decreased.
  4. 04LabIn vitro (cells)Safety outcome

    In normal cells, exendin‑4 did not affect colony formation.

    In plain wordsIn cells, exendin‑4 did not change normal cell colony formation.

    Peptide
    Exenatide (exendin-4)
    Outcome
    No significant changeNormal cell colony formation
    Study

    n: not reported in source

    Normal cells
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    However, GLP-1, HuCCTI or Exendin-4 did not affect the colony of normal cells.
  5. 05LabIn vitro (cells)Efficacy outcome

    In cholangiocarcinoma cells, oxaliplatin plus exendin‑4 had a stronger combined effect than oxaliplatin alone.

    In plain wordsIn cells, oxaliplatin plus exendin‑4 had a stronger combined effect than oxaliplatin alone.

    Peptide
    Exenatide (exendin-4)
    Outcome
    IncreaseCombined inhibitory effect versus oxaliplatin
    Study

    humann: not reported in source

    Cholangiocarcinoma cells
    Comparator
    Oxaliplatin alone
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The combined effect is stronger than that of oxaliplatin or Exendin-4.
    1 context sentence
    Summary · context
    Combination treatment with oxaliplatin and Exendin-4 can significantly inhibit tumor cells' proliferation and promote apoptosis.

Studies that did not test the peptide alone4

In this study, Exenatide (exendin-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Exenatide (exendin-4).

  1. 06AnimalNot a test of Exenatide (exendin-4) aloneAnimal study (in vivo)Mechanism

    In mouse tumors, oxaliplatin plus exendin‑4 increased Caspase3 positive expression.

    In plain wordsIn mice, oxaliplatin plus exendin‑4 increased tumor Caspase3 expression.

    Peptide
    Exenatide (exendin-4)
    Outcome
    IncreaseCaspase3 positive expression
    Study

    mousen: not reported in source

    Nude mice with subcutaneous tumors
    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    In addition, Combination treatment with oxaliplatin and Exendin4 can significantly increase Caspase3 protein positive expression.
    1 context sentence
    Summary · context
    Combination treatment with oxaliplatin and Exendin4 can significantly decrease Ki67 and PCNA proteins' expression in subcutaneous tumors of nude mice.

    In this study, Exenatide (exendin-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Exenatide (exendin-4).

  2. 07LabNot a test of Exenatide (exendin-4) aloneIn vitro (cells)Efficacy outcome

    In cholangiocarcinoma cells, oxaliplatin plus exendin‑4 inhibited proliferation.

    In plain wordsIn cells, oxaliplatin plus exendin‑4 reduced tumor cell multiplication.

    Peptide
    Exenatide (exendin-4)
    Outcome
    DecreaseCell proliferation with combination
    Study

    humann: not reported in source

    Cholangiocarcinoma cells
    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    Combination treatment with oxaliplatin and Exendin-4 can significantly inhibit tumor cells' proliferation and promote apoptosis.
    1 context sentence
    Summary · context
    In vitro tests, including acute toxicity test, cell colony formation assays, cells proliferation and apoptosis, transwell assay, were performed.

    In this study, Exenatide (exendin-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Exenatide (exendin-4).

  3. 08LabNot a test of Exenatide (exendin-4) aloneIn vitro (cells)Efficacy outcome

    In cholangiocarcinoma cells, oxaliplatin plus exendin‑4 promoted apoptosis.

    In plain wordsIn cells, oxaliplatin plus exendin‑4 increased tumor cell death.

    Peptide
    Exenatide (exendin-4)
    Outcome
    IncreaseApoptosis with combination
    Study

    humann: not reported in source

    Cholangiocarcinoma cells
    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    Combination treatment with oxaliplatin and Exendin-4 can significantly inhibit tumor cells' proliferation and promote apoptosis.
    1 context sentence
    Summary · context
    In vitro tests, including acute toxicity test, cell colony formation assays, cells proliferation and apoptosis, transwell assay, were performed.

    In this study, Exenatide (exendin-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Exenatide (exendin-4).

  4. 09LabNot a test of Exenatide (exendin-4) aloneIn vitro (cells)Efficacy outcome

    In cholangiocarcinoma cells, oxaliplatin plus exendin‑4 had a stronger combined effect than exendin‑4 alone.

    In plain wordsIn cells, oxaliplatin plus exendin‑4 had a stronger combined effect than exendin‑4 alone.

    Peptide
    Exenatide (exendin-4)
    Outcome
    IncreaseCombined inhibitory effect versus exendin-4
    Study

    humann: not reported in source

    Cholangiocarcinoma cells
    Comparator
    Exendin-4 alone
    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    The combined effect is stronger than that of oxaliplatin or Exendin-4.
    1 context sentence
    Summary · context
    Combination treatment with oxaliplatin and Exendin-4 can significantly inhibit tumor cells' proliferation and promote apoptosis.

    In this study, Exenatide (exendin-4) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Exenatide (exendin-4).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    22 Oct 2026, 02:26 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 18:59 UTC

    First added · source update from Europe PMC

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Terms as the source lists them.

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