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Study · Not a test of the peptide alone · 2014

5 results

Inactive C8A‑humanin analog is as stable as a potent S14G‑humanin analog.

Arakawa T, Niikura T, Kita Y

Molecular medicine reports14 Nov 2013Cited by 2 (Europe PMC)

PMID24247787 (opens in a new tab)DOI10.3892/mmr.2013.1797 (opens in a new tab)PepBankPUB-24247787
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 01LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism

    By circular dichroism in phosphate-buffered saline, the humanin analogs showed a similar disordered structure at 5˚C but different structures at 37˚C.

    In plain wordsIn lab tests, humanin analogs had a similar loose structure when cold but different structures at the higher temperature.

    Peptide
    Humanin
    Outcome
    Not applicableSecondary structure of HN analogs at low and higher temperature
    Study

    n: not reported in source

    phosphate-buffered saline, circular dichroism
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    While all these analogs of HN demonstrated a similar disordered secondary structure in phosphate-buffered saline at 5˚C, as determined by circular dichroism spectroscopy, they revealed different structures at 37˚C.
    1 context sentence
    Summary · context
    In the present study, the structure and stability of another inactive analog, C8A‑HN, was measured, which has been revealed to have no neuroprotective activity similar to that of the S7A‑HN analog and hence may have compromised stability.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  2. 02LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism

    At 37˚C, less active humanin and inactive S7A-HN converted from a disordered structure to a β-sheet with a valley at about 217 nm.

    In plain wordsIn lab tests, the less active and the inactive humanin forms shifted from a loose structure to a sheet-like structure at the higher temperature.

    Peptide
    Humanin
    Outcome
    Not applicableStructure of less active HN and S7A-HN at 37˚C
    Study

    n: not reported in source

    phosphate-buffered saline, circular dichroism
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    CD spectral valley
    ≈ 217 nm
    HN and S7A-HN at 37˚C

    Source sentences

    Summary
    At 37˚C, less active HN and inactive S7A‑HN revealed a structure with a valley at ~217 nm, indicating a conversion from the disordered structure to a β‑sheet.
    1 context sentence
    Summary · context
    In the present study, the structure and stability of another inactive analog, C8A‑HN, was measured, which has been revealed to have no neuroprotective activity similar to that of the S7A‑HN analog and hence may have compromised stability.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  3. 03LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism

    The conversion of HN and S7A-HN from a disordered structure to a β-sheet was largely irreversible.

    In plain wordsIn lab tests, the change to a sheet-like structure in these humanin forms was mostly not reversible.

    Peptide
    Humanin
    Outcome
    Not applicableReversibility of the conversion to β-sheet
    Study

    n: not reported in source

    phosphate-buffered saline, circular dichroism
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Such a conversion was largely irreversible.
    2 context sentences
    Summary · context
    At 37˚C, less active HN and inactive S7A‑HN revealed a structure with a valley at ~217 nm, indicating a conversion from the disordered structure to a β‑sheet.
    Summary · context
    In the present study, the structure and stability of another inactive analog, C8A‑HN, was measured, which has been revealed to have no neuroprotective activity similar to that of the S7A‑HN analog and hence may have compromised stability.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  4. 04LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism

    C8A-HN and S14G-HN showed a similar structure at 37˚C and at 5˚C and remained largely disordered.

    In plain wordsIn lab tests, the C8A and S14G humanin analogs stayed loose in structure at both temperatures.

    Peptide
    Humanin
    Outcome
    Not applicableStructure of C8A-HN and S14G-HN at 37˚C
    Study

    n: not reported in source

    phosphate-buffered saline, circular dichroism
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    By contrast, C8A‑HN and S14G‑HN demonstrated a similar structure at 37˚C and at 5˚C and remained largely disordered.
    1 context sentence
    Summary · context
    In the present study, the structure and stability of another inactive analog, C8A‑HN, was measured, which has been revealed to have no neuroprotective activity similar to that of the S7A‑HN analog and hence may have compromised stability.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  5. 05LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism

    The small, reversible structural changes of C8A-HN at 37˚C do not support the hypothesis that instability at 37˚C caused its reduced activity.

    In plain wordsIn lab tests, the inactive C8A humanin analog changed little in structure, so instability probably does not explain its lost activity.

    Peptide
    Humanin
    Outcome
    Not applicableStructural change of C8A-HN at 37˚C and reversibility
    Study

    n: not reported in source

    phosphate-buffered saline, circular dichroism
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The observed small structural changes of the C8A‑HN analog at 37˚C and its reversibility upon cooling do not support a hypothesis that the instability at 37˚C may have caused the reduced activity of this analog.
    1 context sentence
    Summary · context
    In the present study, the structure and stability of another inactive analog, C8A‑HN, was measured, which has been revealed to have no neuroprotective activity similar to that of the S7A‑HN analog and hence may have compromised stability.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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