In C2C12 myotubes, preincubation with antioxidants including SS31 (pooled with tempol, NAC and mito-tempol) significantly decreased long-lived protein degradation.
In plain wordsIn lab-grown muscle cells, a group of antioxidants that included SS31 slowed protein breakdown; SS31 alone was not reported.
- Peptide
- Elamipretide (SS-31)
- Outcome
- DecreaseLong-lived protein degradation
- Study
n: not reported in source
Differentiated C2C12 myotubes- Outcome group
- Muscle injury and repair · Musculoskeletal
Source sentences
Preincubation with the general antioxidants tempol (superoxide dismutase mimic) and N-acetyl cysteine (NAC) or the mitochondria-specific antioxidants mito-tempol and SS31 significantly decreased the rates of long-lived protein degradation and LC3B flux and blocked the induction of autophagy-related gene expression.
2 context sentences(design, population, supporting numbers)
To evaluate the effects of physiological levels of mitochondrial-derived reactive oxygen species (ROS) on skeletal muscle autophagy, a proteolytic pathway designed to regulate contractile and myofilament homeostasis and to recycle long-lived proteins and damaged organelles.
Basal levels of autophagy and autophagy triggered by 1.5 to 4 h of acute nutrient deprivation, rapamycin treatment, or leucine deprivation were measured in differentiated C2C12 myotubes using long-lived protein degradation assays, LC3B lipidation, autophagy-related gene expression, and electron microscopy.
In this study, Elamipretide (SS-31) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Elamipretide (SS-31).