Mitochondria-targeted antioxidant peptide SS31 prevents hypoxia/reoxygenation-induced apoptosis by down-regulating p66Shc in renal tubular epithelial cells.

Zhao WY, Han S, Zhang L, Zhu YH, Wang LM, Zeng L

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology6 Sept 2013Cited by 35 (Europe PMC)

PMID24021885DOI10.1159/000354463PepBankPUB-24021885

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Summary

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Text rights: CC BY-NC. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In rat renal tubular cells exposed to hypoxia/reoxygenation, SS31 pretreatment prevented cell death.
In plain wordsIn rat kidney cells, SS31 pretreatment protected against cell death caused by low oxygen followed by reoxygenation.
Peptide
Elamipretide (SS-31)
Outcome
DecreaseCell death after hypoxia/reoxygenation
Study
ratn: not reported in source
NRK52E renal proximal tubular cells, hypoxia/reoxygenation
Comparator
No SS31 pretreatment
Outcome group
Cell viability and death · Kidney and urinary
Studied dose
Dose not stated · added to culture mediumAs given in this study (rat).
Source sentences
Summary
Pretreatment with SS31 or overexpression of a dominantnegative Ser36 mutant p66Shc (p66Shc S36A) or p66Shc siRNA prevented cell death, whereas the protection effect of SS31 was completely blocked by overexpression of wild-type p66Shc.
2 context sentences(design, population, supporting numbers)
Summary · context
The cultured rat renal proximal tubular cell line NRK52E cells were exposed to 24 h hypoxia (5% CO2, 1% O2, 94% N2) followed by 6 h reoxygenation (5% CO2, 21% O2, 74% N2).
Summary · context
SS31 was added to the culture medium 4 h prior to the treatment.
Report a problemFND-2402188501
02LabIn vitro (cells)Mechanism
In rat renal tubular cells, overexpression of wild-type p66Shc completely blocked the protective effect of SS31.
In plain wordsIn rat kidney cells, adding extra normal p66Shc fully blocked the protection that SS31 gave.
Peptide
Elamipretide (SS-31)
Outcome
No significant changeSS31 protection with wild-type p66Shc overexpression
Study
ratn: not reported in source
NRK52E renal proximal tubular cells, hypoxia/reoxygenation
Comparator
No SS31 pretreatment
Outcome group
Receptor activity and mechanism · Kidney and urinary
Studied dose
Dose not stated · added to culture mediumAs given in this study (rat).
Source sentences
Summary
the protection effect of SS31 was completely blocked by overexpression of wild-type p66Shc.
2 context sentences(design, population, supporting numbers)
Summary · context
The cultured rat renal proximal tubular cell line NRK52E cells were exposed to 24 h hypoxia (5% CO2, 1% O2, 94% N2) followed by 6 h reoxygenation (5% CO2, 21% O2, 74% N2).
Summary · context
SS31 was added to the culture medium 4 h prior to the treatment.
Report a problemFND-2402188502
03LabIn vitro (cells)Mechanism
In rat renal tubular cells, SS31 pretreatment reduced hypoxia/reoxygenation-induced intracellular oxidative stress.
In plain wordsIn rat kidney cells, SS31 pretreatment lowered oxidative stress caused by low oxygen followed by reoxygenation.
Peptide
Elamipretide (SS-31)
Outcome
DecreaseIntracellular oxidative stress after hypoxia/reoxygenation
Study
ratn: not reported in source
NRK52E renal proximal tubular cells, hypoxia/reoxygenation
Comparator
No SS31 pretreatment
Outcome group
Oxidative stress · Kidney and urinary
Studied dose
Dose not stated · added to culture mediumAs given in this study (rat).
Source sentences
Summary
Furthermore, SS31 pretreatment reduced H/R-induced intracellular oxidative stress, cytochrome c translocation to the cytoplasm, and caspase-3 activation through inhibiting p66Shc.
2 context sentences(design, population, supporting numbers)
Summary · context
The cultured rat renal proximal tubular cell line NRK52E cells were exposed to 24 h hypoxia (5% CO2, 1% O2, 94% N2) followed by 6 h reoxygenation (5% CO2, 21% O2, 74% N2).
Summary · context
SS31 was added to the culture medium 4 h prior to the treatment.
Report a problemFND-2402188503
04LabIn vitro (cells)Mechanism
In rat renal tubular cells, SS31 pretreatment reduced hypoxia/reoxygenation-induced cytochrome c translocation to the cytoplasm.
In plain wordsIn rat kidney cells, SS31 pretreatment reduced the release of cytochrome c, a cell-death signal, after low oxygen and reoxygenation.
Peptide
Elamipretide (SS-31)
Outcome
DecreaseCytochrome c translocation to cytoplasm
Study
ratn: not reported in source
NRK52E renal proximal tubular cells, hypoxia/reoxygenation
Comparator
No SS31 pretreatment
Outcome group
Cell viability and death · Kidney and urinary
Studied dose
Dose not stated · added to culture mediumAs given in this study (rat).
Source sentences
Summary
Furthermore, SS31 pretreatment reduced H/R-induced intracellular oxidative stress, cytochrome c translocation to the cytoplasm, and caspase-3 activation through inhibiting p66Shc.
2 context sentences(design, population, supporting numbers)
Summary · context
The cultured rat renal proximal tubular cell line NRK52E cells were exposed to 24 h hypoxia (5% CO2, 1% O2, 94% N2) followed by 6 h reoxygenation (5% CO2, 21% O2, 74% N2).
Summary · context
SS31 was added to the culture medium 4 h prior to the treatment.
Report a problemFND-2402188504
05LabIn vitro (cells)Mechanism
In rat renal tubular cells, SS31 pretreatment reduced hypoxia/reoxygenation-induced caspase-3 activation.
In plain wordsIn rat kidney cells, SS31 pretreatment reduced activation of caspase-3, a cell-death enzyme, after low oxygen and reoxygenation.
Peptide
Elamipretide (SS-31)
Outcome
DecreaseCaspase-3 activation
Study
ratn: not reported in source
NRK52E renal proximal tubular cells, hypoxia/reoxygenation
Comparator
No SS31 pretreatment
Outcome group
Cell viability and death · Kidney and urinary
Studied dose
Dose not stated · added to culture mediumAs given in this study (rat).
Source sentences
Summary
Furthermore, SS31 pretreatment reduced H/R-induced intracellular oxidative stress, cytochrome c translocation to the cytoplasm, and caspase-3 activation through inhibiting p66Shc.
2 context sentences(design, population, supporting numbers)
Summary · context
The cultured rat renal proximal tubular cell line NRK52E cells were exposed to 24 h hypoxia (5% CO2, 1% O2, 94% N2) followed by 6 h reoxygenation (5% CO2, 21% O2, 74% N2).
Summary · context
SS31 was added to the culture medium 4 h prior to the treatment.
Report a problemFND-2402188505

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 08:19 UTC
Study results updated
Version 1
9 Oct 2026, 02:47 UTC
First added · source update from Europe PMC

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Mitochondria-targeted antioxidant peptide SS31 prevents hypoxia/reoxygenation-induced apoptosis by down-regulating p66Shc in renal tubular epithelial cells.

Summary

Results from this study5

Version history

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