Skip to content

Study · In vitro (cells) · rat · 2013

8 results

A humanin analog decreases oxidative stress and preserves mitochondrial integrity in cardiac myoblasts.

Klein LE, Cui L, Gong Z, Su K, Muzumdar R

Biochemical and biophysical research communications25 Aug 2013Cited by 81 (Europe PMC)

PMID23985350 (opens in a new tab)PMCIDPMC3853355 (opens in a new tab)DOI10.1016/j.bbrc.2013.08.055 (opens in a new tab)PepBankPUB-23985350
Source linkPubMedAsk about this study
Jump to 8 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In H9C2 cardiac myoblasts exposed to H2O2, HNG reduced intracellular reactive oxygen species.

    In plain wordsIn heart muscle cells, a humanin analog reduced harmful reactive oxygen molecules caused by H2O2.

    Peptide
    Humanin
    Outcome
    DecreaseIntracellular ROS
    Study

    ratn: not reported in source

    H9C2 cardiac myoblasts exposed to H2O2
    Outcome group
    Mitochondrial function · Cardiovascular
    Studied dose
    nanomolar concentrationsAs given in this study (rat).

    Source sentences

    Summary
    Cells treated with HNG in the presence of H2O2 demonstrated reduced intracellular reactive oxygen species (ROS), preserved mitochondrial membrane potential, ATP levels and mitochondrial structure.
    1 context sentence
    Summary · context
    Rat cardiac myoblasts (H9C2 cells) were exposed to nanomolar concentrations of HNG and to hydrogen peroxide (H2O2).
  2. 02LabIn vitro (cells)Mechanism

    In H9C2 cardiac myoblasts exposed to H2O2, HNG preserved mitochondrial membrane potential.

    In plain wordsIn heart muscle cells, a humanin analog kept mitochondrial membrane potential from falling under H2O2.

    Peptide
    Humanin
    Outcome
    IncreaseMitochondrial membrane potential
    Study

    ratn: not reported in source

    H9C2 cardiac myoblasts exposed to H2O2
    Outcome group
    Mitochondrial function · Cardiovascular
    Studied dose
    nanomolar concentrationsAs given in this study (rat).

    Source sentences

    Summary
    Cells treated with HNG in the presence of H2O2 demonstrated reduced intracellular reactive oxygen species (ROS), preserved mitochondrial membrane potential, ATP levels and mitochondrial structure.
    1 context sentence
    Summary · context
    Rat cardiac myoblasts (H9C2 cells) were exposed to nanomolar concentrations of HNG and to hydrogen peroxide (H2O2).
  3. 03LabIn vitro (cells)Mechanism

    In H9C2 cardiac myoblasts exposed to H2O2, HNG preserved ATP levels.

    In plain wordsIn heart muscle cells, a humanin analog kept ATP energy levels from falling under H2O2.

    Peptide
    Humanin
    Outcome
    IncreaseATP levels
    Study

    ratn: not reported in source

    H9C2 cardiac myoblasts exposed to H2O2
    Outcome group
    Mitochondrial function · Cardiovascular
    Studied dose
    nanomolar concentrationsAs given in this study (rat).

    Source sentences

    Summary
    Cells treated with HNG in the presence of H2O2 demonstrated reduced intracellular reactive oxygen species (ROS), preserved mitochondrial membrane potential, ATP levels and mitochondrial structure.
    1 context sentence
    Summary · context
    Rat cardiac myoblasts (H9C2 cells) were exposed to nanomolar concentrations of HNG and to hydrogen peroxide (H2O2).
  4. 04LabIn vitro (cells)Mechanism

    In H9C2 cardiac myoblasts exposed to H2O2, HNG preserved mitochondrial structure.

    In plain wordsIn heart muscle cells, a humanin analog kept mitochondrial structure intact under H2O2.

    Peptide
    Humanin
    Outcome
    IncreaseMitochondrial structure
    Study

    ratn: not reported in source

    H9C2 cardiac myoblasts exposed to H2O2
    Outcome group
    Mitochondrial function · Cardiovascular
    Studied dose
    nanomolar concentrationsAs given in this study (rat).

    Source sentences

    Summary
    Cells treated with HNG in the presence of H2O2 demonstrated reduced intracellular reactive oxygen species (ROS), preserved mitochondrial membrane potential, ATP levels and mitochondrial structure.
    1 context sentence
    Summary · context
    Rat cardiac myoblasts (H9C2 cells) were exposed to nanomolar concentrations of HNG and to hydrogen peroxide (H2O2).
  5. 05LabIn vitro (cells)Mechanism

    In H9C2 cardiac myoblasts, HNG induced activation of catalase within 5 min.

    In plain wordsIn heart muscle cells, a humanin analog switched on the antioxidant enzyme catalase within 5 minutes.

    Peptide
    Humanin
    Outcome
    IncreaseCatalase activity
    Study

    ratn: not reported in source

    H9C2 cardiac myoblasts exposed to H2O2
    Outcome group
    Mitochondrial function · Cardiovascular
    Studied dose
    nanomolar concentrations · within 5 minAs given in this study (rat).

    Source sentences

    Summary
    HNG induced activation of catalase and glutathione peroxidase (GPx) within 5 min
    1 context sentence
    Summary · context
    Rat cardiac myoblasts (H9C2 cells) were exposed to nanomolar concentrations of HNG and to hydrogen peroxide (H2O2).
  6. 06LabIn vitro (cells)Mechanism

    In H9C2 cardiac myoblasts, HNG induced activation of glutathione peroxidase within 5 min.

    In plain wordsIn heart muscle cells, a humanin analog switched on the antioxidant enzyme glutathione peroxidase within 5 minutes.

    Peptide
    Humanin
    Outcome
    IncreaseGlutathione peroxidase activity
    Study

    ratn: not reported in source

    H9C2 cardiac myoblasts exposed to H2O2
    Outcome group
    Mitochondrial function · Cardiovascular
    Studied dose
    nanomolar concentrations · within 5 minAs given in this study (rat).

    Source sentences

    Summary
    HNG induced activation of catalase and glutathione peroxidase (GPx) within 5 min
    1 context sentence
    Summary · context
    Rat cardiac myoblasts (H9C2 cells) were exposed to nanomolar concentrations of HNG and to hydrogen peroxide (H2O2).
  7. 07LabIn vitro (cells)Mechanism

    In H9C2 cardiac myoblasts, HNG decreased the ratio of oxidized to reduced glutathione within 30 min.

    In plain wordsIn heart muscle cells, a humanin analog lowered the share of oxidized glutathione within 30 minutes.

    Peptide
    Humanin
    Outcome
    DecreaseRatio of oxidized to reduced glutathione
    Study

    ratn: not reported in source

    H9C2 cardiac myoblasts exposed to H2O2
    Outcome group
    Mitochondrial function · Cardiovascular
    Studied dose
    nanomolar concentrations · within 30 minAs given in this study (rat).

    Source sentences

    Summary
    HNG induced activation of catalase and glutathione peroxidase (GPx) within 5 min and decreased the ratio of oxidized to reduced glutathione within 30 min
    1 context sentence
    Summary · context
    Rat cardiac myoblasts (H9C2 cells) were exposed to nanomolar concentrations of HNG and to hydrogen peroxide (H2O2).

Studies that did not test the peptide alone1

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 08LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In H9C2 cardiac myoblasts exposed to H2O2, siRNA knockdown of both Abl and Arg, but neither alone, abolished the HNG-mediated reduction of ROS.

    In plain wordsIn heart muscle cells, switching off both Abl and Arg removed the ROS reduction that a humanin analog caused.

    Peptide
    Humanin
    Outcome
    No significant changeHNG-mediated ROS reduction after Abl and Arg knockdown
    Study

    ratn: not reported in source

    H9C2 cardiac myoblasts exposed to H2O2
    Outcome group
    Mitochondrial function · Cardiovascular

    Source sentences

    Summary
    siRNA knockdown of both Abl and Arg, but neither alone, abolished the HNG-mediated reduction of ROS in myoblasts exposed to H2O2.
    1 context sentence
    Summary · context
    Rat cardiac myoblasts (H9C2 cells) were exposed to nanomolar concentrations of HNG and to hydrogen peroxide (H2O2).

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.