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Study · In vitro (cells) · 2013

4 results

SH3-binding protein 5 mediates the neuroprotective effect of the secreted bioactive peptide humanin by inhibiting c-Jun NH2-terminal kinase.

Takeshita Y, Hashimoto Y, Nawa M, Uchino H, Matsuoka M

The Journal of biological chemistry16 Jul 2013Cited by 22 (Europe PMC)

PMID23861391 (opens in a new tab)PMCIDPMC3750166 (opens in a new tab)DOI10.1074/jbc.m113.469692 (opens in a new tab)PepBankPUB-23861391
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Summary

Humanin is a secreted bioactive peptide that suppresses cell toxicity caused by a variety of insults. The neuroprotective effect of Humanin against Alzheimer disease (AD)-related death is mediated by the binding of Humanin to its heterotrimeric Humanin receptor composed of ciliary neurotrophic receptor α, WSX-1, and gp130, as well as the activation of intracellular signaling pathways including a JAK2 and STAT3 signaling axis. Despite the elucidation of the signaling pathways by which Humanin mediates its neuroprotection, the transcriptional targets of Humanin that behaves as effectors of Humanin remains undefined. In the present study, Humanin increased the mRNA and protein expression of SH3 domain-binding protein 5 (SH3BP5), which has been known to be a JNK interactor, in neuronal cells. Similar to Humanin treatment, overexpression of SH3BP5 inhibited AD-related neuronal death, while siRNA-mediated knockdown of endogenous SH3BP5 expression attenuated the neuroprotective effect of Humanin. These results indicate that SH3BP5 is a downstream effector of Humanin. Furthermore, biochemical analysis has revealed that SH3BP5 binds to JNK and directly inhibits JNK through its two putative mitogen-activated protein kinase interaction motifs (KIMs).
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In neuronal cells, humanin increased the mRNA expression of SH3 domain-binding protein 5 (SH3BP5).

    In plain wordsIn neuronal cells, humanin raised the activity of the gene for SH3BP5.

    Peptide
    Humanin
    Outcome
    IncreaseSH3BP5 mRNA expression
    Study

    n: not reported in source

    Neuronal cells
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In the present study, Humanin increased the mRNA and protein expression of SH3 domain-binding protein 5 (SH3BP5), which has been known to be a JNK interactor, in neuronal cells.
  2. 02LabIn vitro (cells)Mechanism

    In neuronal cells, humanin increased the protein expression of SH3 domain-binding protein 5 (SH3BP5).

    In plain wordsIn neuronal cells, humanin raised the amount of SH3BP5 protein.

    Peptide
    Humanin
    Outcome
    IncreaseSH3BP5 protein expression
    Study

    n: not reported in source

    Neuronal cells
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In the present study, Humanin increased the mRNA and protein expression of SH3 domain-binding protein 5 (SH3BP5), which has been known to be a JNK interactor, in neuronal cells.

Studies that did not test the peptide alone2

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 03LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In neuronal cells, overexpression of SH3BP5 inhibited AD-related neuronal death, similar to humanin treatment.

    In plain wordsIn neuronal cells, making cells produce more SH3BP5 reduced Alzheimer-related cell death, as humanin did.

    Peptide
    Humanin
    Outcome
    DecreaseAD-related neuronal death with SH3BP5 overexpression
    Study

    n: not reported in source

    Neuronal cells
    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    Similar to Humanin treatment, overexpression of SH3BP5 inhibited AD-related neuronal death

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  2. 04LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In neuronal cells, siRNA-mediated knockdown of endogenous SH3BP5 expression attenuated the neuroprotective effect of humanin.

    In plain wordsIn neuronal cells, switching off SH3BP5 weakened the protection that humanin gave.

    Peptide
    Humanin
    Outcome
    DecreaseNeuroprotective effect of humanin after SH3BP5 knockdown
    Study

    n: not reported in source

    Neuronal cells
    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    while siRNA-mediated knockdown of endogenous SH3BP5 expression attenuated the neuroprotective effect of Humanin.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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