Quantitative analysis of thymosin β4 in whole saliva by capillary electrophoresis–mass spectrometry using multiple ions monitoring (CE-MIM-MS.).

Rossetti DV, Martelli C, Longhi R, Iavarone F, Castagnola M, Desiderio C

Electrophoresis1 Sept 2013Cited by 9 (Europe PMC)

PMID23857244DOI10.1002/elps.201300165PepBankPUB-23857244

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01HumanEndogenous levelObservational studyOther
In whole saliva, the body's own Tβ4 content varied between individuals and across days in the same individual.
In plain wordsThe body's own Tβ4 level in saliva differed between people and between days in the same person.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableTβ4 concentration in whole saliva
Study
n: not reported in source
Outcome group
Endogenous peptide levels · Whole body
Measurements
Salivary Tβ4 concentration
0.3 to 1.4 μM
whole saliva
Source sentences
Summary
It was successfully applied to the analysis of Tβ4 in whole saliva showing a variable peptide content from individual to individual (in the range of 0.3–1.4 μM) and in different days from the same individual.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-2385724403

Studies that did not test the peptide alone2

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

02LabNot a test of Thymosin beta-4 (human) aloneOther designOther
A CE-MS multiple ions monitoring method for Tβ4 showed linear response from the lower limit of quantification to 4 μM.
In plain wordsIn lab tests, a mass spectrometry method measured Tβ4 in a straight-line response across a set range.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableCE-MS method linearity and lower limit of quantification
Study
n: not reported in source
CE-MS assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Lower limit of quantification
0.25 μM
CE-MIM-MS
Upper limit of linear range
4 μM
CE-MIM-MS
Average R2
0.996
CE-MIM-MS
Source sentences
Summary
The method was validated for quantitation showing linearity of the response in the range 0.25 (lower limit of quantification) to 4 μM (average R2 0.996 ± 0.005) and intra- and interassay precision and accuracy at three different concentrations with RSD values in the range of 7–16%.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2385724401
03LabNot a test of Thymosin beta-4 (human) aloneOther designOther
The CE-MS method had intra- and interassay precision and accuracy at three concentrations, with RSD values of 7-16%.
In plain wordsIn lab tests, the mass spectrometry method gave repeatable results for Tβ4 at three concentrations.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableCE-MS method precision
Study
n: not reported in source
CE-MS assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
RSD
7 to 16%
intra- and interassay
Source sentences
Summary
The method was validated for quantitation showing linearity of the response in the range 0.25 (lower limit of quantification) to 4 μM (average R2 0.996 ± 0.005) and intra- and interassay precision and accuracy at three different concentrations with RSD values in the range of 7–16%.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2385724402

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

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