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Study · In vitro (cells) · 2013

6 results

Bacterial expression, purification and angiogenesis-promoting activity of human thymosin β4.

Kozaczuk A, Selmi A, Bednarek R

Protein expression and purification11 Jun 2013Cited by 5 (Europe PMC)

PMID23769831 (opens in a new tab)DOI10.1016/j.pep.2013.06.003 (opens in a new tab)PepBankPUB-23769831
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In an in vitro endothelial cell model, recombinant Tβ4 stimulated angiogenesis through activation of the endothelial proteolytic systems.

    In plain wordsIn lab tests with endothelial cells, recombinant Tβ4 stimulated new blood vessel formation by activating the cells' protein-cutting systems.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseAngiogenesis via endothelial proteolytic systems
    Study

    n: not reported in source

    in vitro endothelial cell model
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Functional studies revealed that recombinant Tβ4 stimulated angiogenesis via activation of the endothelial proteolytic systems, inhibition of endothelial cell adhesion, promotion of migration and capillary tube formation in Matrigel, and that its activity was similar to that observed for the synthetic peptide.
    1 context sentence
    Summary · context
    The presented study comprises the first evidence that recombinant Tβ4 promotes angiogenesis in an in vitro endothelial cell model.
  2. 02LabIn vitro (cells)Activity measurement

    In an in vitro endothelial cell model, recombinant Tβ4 inhibited endothelial cell adhesion.

    In plain wordsIn lab tests with endothelial cells, recombinant Tβ4 reduced how well the cells stuck to surfaces.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseEndothelial cell adhesion
    Study

    n: not reported in source

    in vitro endothelial cell model
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Functional studies revealed that recombinant Tβ4 stimulated angiogenesis via activation of the endothelial proteolytic systems, inhibition of endothelial cell adhesion, promotion of migration and capillary tube formation in Matrigel, and that its activity was similar to that observed for the synthetic peptide.
  3. 03LabIn vitro (cells)Activity measurement

    In an in vitro endothelial cell model, recombinant Tβ4 promoted endothelial cell migration.

    In plain wordsIn lab tests with endothelial cells, recombinant Tβ4 made the cells move more.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseEndothelial cell migration
    Study

    n: not reported in source

    in vitro endothelial cell model
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Functional studies revealed that recombinant Tβ4 stimulated angiogenesis via activation of the endothelial proteolytic systems, inhibition of endothelial cell adhesion, promotion of migration and capillary tube formation in Matrigel, and that its activity was similar to that observed for the synthetic peptide.
  4. 04LabIn vitro (cells)Activity measurement

    In an in vitro endothelial cell model, recombinant Tβ4 promoted capillary tube formation in Matrigel.

    In plain wordsIn lab tests with endothelial cells, recombinant Tβ4 helped the cells form tube-like structures.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseCapillary tube formation in Matrigel
    Study

    n: not reported in source

    in vitro endothelial cell model
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Functional studies revealed that recombinant Tβ4 stimulated angiogenesis via activation of the endothelial proteolytic systems, inhibition of endothelial cell adhesion, promotion of migration and capillary tube formation in Matrigel, and that its activity was similar to that observed for the synthetic peptide.
  5. 05LabIn vitro (cells)Activity measurement

    In an in vitro endothelial cell model, the angiogenic activity of recombinant Tβ4 was similar to that of the synthetic peptide.

    In plain wordsIn lab tests with endothelial cells, recombinant Tβ4 worked about like the synthetic peptide.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeActivity of recombinant versus synthetic Tβ4
    Study

    n: not reported in source

    in vitro endothelial cell model
    Comparator
    Synthetic Tβ4
    Outcome group
    Angiogenesis · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Functional studies revealed that recombinant Tβ4 stimulated angiogenesis via activation of the endothelial proteolytic systems, inhibition of endothelial cell adhesion, promotion of migration and capillary tube formation in Matrigel, and that its activity was similar to that observed for the synthetic peptide.

Studies that did not test the peptide alone1

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 06LabNot a test of Thymosin beta-4 (human) aloneOther designOther

    Recombinant, non-glycosylated Tβ4 was overexpressed in soluble form in E. coli and purified by two-step immobilized metal ion affinity chromatography.

    In plain wordsIn lab tests, bacteria made Tβ4 in soluble form, and two chromatography steps purified it.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableBacterial expression and purification of Tβ4
    Study

    n: not reported in source

    E. coli BL21(DE3)
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    The recombinant, non-glycosylated peptide was overexpressed in soluble form and purified by two-step immobilized metal ion affinity chromatography.
    1 context sentence
    Summary · context
    To overcome the high costs of solid phase synthesis, we developed a genetic engineering procedure of Tβ4 expression in a protease-deficient host strain, Escherichia coli BL21(DE3), transformed with different expression vectors (pRSETA, pET-15b and pEcoli-Cterm6 × HN).

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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