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Study · In vitro (cells) · 2013

5 results

Production and characterization of highly purified recombinant thymosin beta 4 in Escherichia coli.

Li T, Ma SY, Tang XC, Nie LY, Huang H

Protein expression and purification24 May 2013Cited by 8 (Europe PMC)

PMID23711379 (opens in a new tab)DOI10.1016/j.pep.2013.05.006 (opens in a new tab)PepBankPUB-23711379
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In the E-rosette test, the bioactivity of purified recombinant Tβ4 was consistent with other published work.

    In plain wordsIn a lab test, purified recombinant Tβ4 showed activity in line with other published work.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableBioactivity in E-rosette test
    Study

    n: not reported in source

    E-rosette test
    Outcome group
    Immune response · Immune and inflammation
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The E-rosette test demonstrated that the bioactivity of purified Tβ4 was consistent with other published work.
    1 context sentence
    Summary · context
    It was expressed in a soluble form using a DsbA and hexahistindine tag in Escherichia coli (E. coli).

Studies that did not test the peptide alone4

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 02LabNot a test of Thymosin beta-4 (human) aloneOther designOther

    High cell density cultivation of E. coli reached a final biomass of about 50 g per litre dry cell weight.

    In plain wordsIn lab production, bacteria reached a final biomass of about 50 g per litre dry cell weight.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableBiomass concentration in high cell density cultivation
    Study

    n: not reported in source

    E. coli expression and purification
    Outcome group
    Product quality and purity · Pharmacology

    Measurements

    Final biomass concentration
    ≈ 50 g L(-1)
    dry cell weight

    Source sentences

    Summary
    Using high cell density cultivation, the final biomass concentration was about 50 g L(-1) dry cell weight with the expression level of the fusion protein being 40%.
    1 context sentence
    Summary · context
    It was expressed in a soluble form using a DsbA and hexahistindine tag in Escherichia coli (E. coli).

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  2. 03LabNot a test of Thymosin beta-4 (human) aloneOther designOther

    The expression level of the Tβ4 fusion protein was 40%.

    In plain wordsIn lab production, the Tβ4 fusion protein made up 40% of the expression level.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableFusion protein expression level
    Study

    n: not reported in source

    E. coli expression and purification
    Outcome group
    Product quality and purity · Pharmacology

    Measurements

    Fusion protein expression level
    40%
    E. coli

    Source sentences

    Summary
    Using high cell density cultivation, the final biomass concentration was about 50 g L(-1) dry cell weight with the expression level of the fusion protein being 40%.
    1 context sentence
    Summary · context
    It was expressed in a soluble form using a DsbA and hexahistindine tag in Escherichia coli (E. coli).

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  3. 04LabNot a test of Thymosin beta-4 (human) aloneOther designOther

    After five-step column purification, recombinant Tβ4 purity was above 98%.

    In plain wordsIn lab production, purified recombinant Tβ4 was above 98% pure.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicablePurity of recombinant Tβ4
    Study

    n: not reported in source

    E. coli expression and purification
    Outcome group
    Product quality and purity · Pharmacology

    Measurements

    Purity
    > 98%
    recombinant Tβ4

    Source sentences

    Summary
    The purity of Tβ4 was above 98% and all the host cell related impurities, such as endotoxin, host cell protein and residual DNA levels, were within the permissible range listed in the Chinese Pharmacopoeia.
    1 context sentence
    Summary · context
    It was expressed in a soluble form using a DsbA and hexahistindine tag in Escherichia coli (E. coli).

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  4. 05LabNot a test of Thymosin beta-4 (human) aloneOther designOther

    Endotoxin, host cell protein and residual DNA levels were within the permissible range of the Chinese Pharmacopoeia.

    In plain wordsIn lab production, impurity levels in the purified Tβ4 were within the permitted range.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableHost cell impurities (endotoxin, host cell protein, residual DNA)
    Study

    n: not reported in source

    E. coli expression and purification
    Outcome group
    Product quality and purity · Pharmacology

    Source sentences

    Summary
    The purity of Tβ4 was above 98% and all the host cell related impurities, such as endotoxin, host cell protein and residual DNA levels, were within the permissible range listed in the Chinese Pharmacopoeia.
    1 context sentence
    Summary · context
    It was expressed in a soluble form using a DsbA and hexahistindine tag in Escherichia coli (E. coli).

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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