Nanog suppresses cell migration by downregulating thymosin β4 and Rnd3.

Zhou Y, Li S, Huang Q, Xie L, Zhu X

Journal of molecular cell biology16 Jan 2013Cited by 14 (Europe PMC)

PMID23329853DOI10.1093/jmcb/mjt002PepBankPUB-23329853

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Natural levels measured2

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01LabEndogenous levelIn vitro (cells)Other
In mouse embryonic stem cells, Tβ4 and Rnd3 were upregulated during differentiation.
In plain wordsIn mouse stem cells, the cells' own Tβ4 levels rose as the cells matured.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseTβ4 expression during ES cell differentiation
Study
mousen: not reported in source
mouse embryonic stem cells
Outcome group
Endogenous peptide levels · Whole body
Source sentences
Summary
Both proteins were upregulated during mouse ES cell differentiation.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-2332985304
02LabEndogenous levelIn vitro (cells)Other
In pluripotent mouse P19 cells, Tβ4 levels increased upon Nanog ablation, with an increase in cell motility.
In plain wordsIn mouse cells, the cells' own Tβ4 levels rose when Nanog was removed.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseTβ4 level after Nanog ablation
Study
mousen: not reported in source
pluripotent mouse P19 cells
Outcome group
Endogenous peptide levels · Whole body
Source sentences
Summary
Their levels in the pluripotent mouse P19 cells also increased upon Nanog ablation, coincident with an increase in cell motility.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-2332985305

Studies that did not test the peptide alone3

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In ECV304 cells, RNAi depletion of Tβ4 phenocopied the migration inhibition caused by Nanog.
In plain wordsIn cells, lowering Tβ4 reduced cell movement in the same way as Nanog did.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseCell migration after Tβ4 depletion
Study
n: not reported in source
ECV304 cells
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Whole body
Source sentences
Summary
Their depletions in ECV304 cells by RNAi phenocopied the ectopic expression of Nanog in both cell motility
1 context sentence(design, population, supporting numbers)
Summary · context
We found that the ectopic expression of Nanog, Sox2, or Oct4 markedly inhibited ECV304 cell migration.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2332985301
04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In ECV304 cells, RNAi depletion of Tβ4 phenocopied the actin disorganization caused by Nanog.
In plain wordsIn cells, lowering Tβ4 disturbed the structure of actin fibres in the same way as Nanog did.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseActin organization after Tβ4 depletion
Study
n: not reported in source
ECV304 cells
Comparator
Control cells
Outcome group
Receptor activity and mechanism · Whole body
Source sentences
Summary
Their depletions in ECV304 cells by RNAi phenocopied the ectopic expression of Nanog in both cell motility and actin organization
1 context sentence(design, population, supporting numbers)
Summary · context
Detailed examinations revealed that Nanog induced disorganizations of the actin cytoskeleton and peripheral localizations of focal adhesions.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2332985302
05LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In ECV304 cells, ectopic expression of Tβ4 rescued the migration defect caused by Nanog overexpression.
In plain wordsIn cells, raising Tβ4 levels restored the cell movement that Nanog had reduced.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseRescue of Nanog-induced migration defect by ectopic Tβ4
Study
n: not reported in source
ECV304 cells
Comparator
Nanog overexpression alone
Outcome group
Receptor activity and mechanism · Whole body
Source sentences
Summary
whereas their ectopic expressions rescued the migration defect of Nanog overexpression.
1 context sentence(design, population, supporting numbers)
Summary · context
We found that the ectopic expression of Nanog, Sox2, or Oct4 markedly inhibited ECV304 cell migration.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-2332985303

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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