Immature rat seminal vesicles show histomorphological and ultrastructural alterations following treatment with kisspeptin-10.

Ramzan F, Qureshi IZ, Ramzan M, Ramzan MH, Ramzan F

Reproductive biology and endocrinology : RB&E10 Mar 2012Cited by 9 (Europe PMC)

PMID22404961PMCIDPMC3359248DOI10.1186/1477-7827-10-18PepBankPUB-22404961

Summary

BackgroundDegenerative effects of critical regulators of reproduction, the kisspeptin peptides, on cellular aspects of sexually immature male gonads are known but similar information on accessory sex glands remain elusive.MethodsPrepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day. Control rats were maintained in parallel. The day following the end of the experimental period, seminal vesicles were removed and processed for light and electron microscopic examination using the standard methods. DNA damage was estimated by DNA ladder assay and DNA fragmentation assay.ResultsThe results demonstrated cellular degeneration. Epithelial cell height of seminal vesicles decreased significantly at all doses (P < 0.05). Marked decrease in epithelial folds was readily noticeable, while the lumen was dilated. Ultrastructural changes were characterized by dilatation of endoplasmic reticulum and Golgi complex, heterochromatization of nuclei, invagination of nuclear membranes and a decreased number of secretory granules. Percent DNA damage to the seminal vesicle was 19.54 +/- 1.98, 38.06 +/- 2.09 and 58.18 +/- 2.59 at 10 pg, 1 ng and 1 microgram doses respectively.ConclusionThe study reveals that continuous administration of kisspeptin does not lead to an early maturation but instead severe degeneration of sexually immature seminal vesicles.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In prepubertal rats, 12 days of kisspeptin‑10 reduced the epithelial cell height of seminal vesicles at all three doses.
In plain wordsIn young rats, kisspeptin‑10 for 12 days lowered the height of the cells lining the seminal vesicles at every dose tested.
Peptide
Kisspeptin-10
Outcome
DecreaseSeminal vesicle epithelial cell height
Study
ratn: not reported in source
Prepubertal laboratory rats
Comparator
Control rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10 pg, 1 ng and 1 microgram · 12 daysAs given in this study (rat).
Source sentences
Summary
Epithelial cell height of seminal vesicles decreased significantly at all doses (P < 0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
Prepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day.
Summary · context
Control rats were maintained in parallel.
Show in the study text Report a problemFND-2240496101
02AnimalAnimal study (in vivo)Efficacy outcome
In prepubertal rats, kisspeptin‑10 caused a marked decrease in epithelial folds of the seminal vesicles.
In plain wordsIn young rats, kisspeptin‑10 reduced the folds in the lining of the seminal vesicles.
Peptide
Kisspeptin-10
Outcome
DecreaseSeminal vesicle epithelial folds
Study
ratn: not reported in source
Prepubertal laboratory rats
Comparator
Control rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10 pg, 1 ng and 1 microgram · 12 daysAs given in this study (rat).
Source sentences
Summary
Marked decrease in epithelial folds was readily noticeable
2 context sentences(design, population, supporting numbers)
Summary · context
Prepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day.
Summary · context
Control rats were maintained in parallel.
Show in the study text Report a problemFND-2240496102
03AnimalAnimal study (in vivo)Efficacy outcome
In prepubertal rats, kisspeptin‑10 treatment was followed by a dilated lumen of the seminal vesicles.
In plain wordsIn young rats, kisspeptin‑10 treatment was followed by a widened space inside the seminal vesicles.
Peptide
Kisspeptin-10
Outcome
IncreaseSeminal vesicle lumen dilation
Study
ratn: not reported in source
Prepubertal laboratory rats
Comparator
Control rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10 pg, 1 ng and 1 microgram · 12 daysAs given in this study (rat).
Source sentences
Summary
while the lumen was dilated
2 context sentences(design, population, supporting numbers)
Summary · context
Prepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day.
Summary · context
Control rats were maintained in parallel.
Show in the study text Report a problemFND-2240496103
04AnimalAnimal study (in vivo)Efficacy outcome
In prepubertal rats, kisspeptin‑10 caused dilatation of endoplasmic reticulum and Golgi complex, nuclear changes and fewer secretory granules in seminal vesicle cells.
In plain wordsIn young rats, kisspeptin‑10 changed the inner structure of seminal vesicle cells and reduced their secretory granules.
Peptide
Kisspeptin-10
Outcome
MixedSeminal vesicle ultrastructural changes
Study
ratn: not reported in source
Prepubertal laboratory rats
Comparator
Control rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10 pg, 1 ng and 1 microgram · 12 daysAs given in this study (rat).
Source sentences
Summary
Ultrastructural changes were characterized by dilatation of endoplasmic reticulum and Golgi complex, heterochromatization of nuclei, invagination of nuclear membranes and a decreased number of secretory granules.
2 context sentences(design, population, supporting numbers)
Summary · context
Prepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day.
Summary · context
Control rats were maintained in parallel.
Show in the study text Report a problemFND-2240496104
05AnimalAnimal study (in vivo)Safety outcome
In prepubertal rats, 10 pg kisspeptin‑10 was followed by 19.54% DNA damage in the seminal vesicle.
In plain wordsIn young rats, the lowest kisspeptin‑10 dose was followed by DNA damage in the seminal vesicle.
Peptide
Kisspeptin-10
Outcome
Not applicableSeminal vesicle DNA damage at 10 pg
Study
ratn: not reported in source
Prepubertal laboratory rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10 pg · 12 daysAs given in this study (rat).
Measurements
DNA damage
19.54%
10 pg
Source sentences
Summary
Percent DNA damage to the seminal vesicle was 19.54 +/- 1.98, 38.06 +/- 2.09 and 58.18 +/- 2.59 at 10 pg, 1 ng and 1 microgram doses respectively.
2 context sentences(design, population, supporting numbers)
Summary · context
Prepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day.
Summary · context
Control rats were maintained in parallel.
Show in the study text Report a problemFND-2240496105
06AnimalAnimal study (in vivo)Safety outcome
In prepubertal rats, 1 ng kisspeptin‑10 was followed by 38.06% DNA damage in the seminal vesicle.
In plain wordsIn young rats, the middle kisspeptin‑10 dose was followed by more DNA damage in the seminal vesicle than the lowest dose.
Peptide
Kisspeptin-10
Outcome
Not applicableSeminal vesicle DNA damage at 1 ng
Study
ratn: not reported in source
Prepubertal laboratory rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
1 ng · 12 daysAs given in this study (rat).
Measurements
DNA damage
38.06%
1 ng
Source sentences
Summary
Percent DNA damage to the seminal vesicle was 19.54 +/- 1.98, 38.06 +/- 2.09 and 58.18 +/- 2.59 at 10 pg, 1 ng and 1 microgram doses respectively.
2 context sentences(design, population, supporting numbers)
Summary · context
Prepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day.
Summary · context
Control rats were maintained in parallel.
Show in the study text Report a problemFND-2240496106
07AnimalAnimal study (in vivo)Safety outcome
In prepubertal rats, 1 microgram kisspeptin‑10 was followed by 58.18% DNA damage in the seminal vesicle.
In plain wordsIn young rats, the highest kisspeptin‑10 dose was followed by the most DNA damage in the seminal vesicle.
Peptide
Kisspeptin-10
Outcome
Not applicableSeminal vesicle DNA damage at 1 microgram
Study
ratn: not reported in source
Prepubertal laboratory rats
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
1 microgram · 12 daysAs given in this study (rat).
Measurements
DNA damage
58.18%
1 microgram
Source sentences
Summary
Percent DNA damage to the seminal vesicle was 19.54 +/- 1.98, 38.06 +/- 2.09 and 58.18 +/- 2.59 at 10 pg, 1 ng and 1 microgram doses respectively.
2 context sentences(design, population, supporting numbers)
Summary · context
Prepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day.
Summary · context
Control rats were maintained in parallel.
Show in the study text Report a problemFND-2240496107

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

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PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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