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Study · In vitro (cells) · 2012

8 results

Kisspeptin-10 inhibits cell migration in vitro via a receptor-GSK3 beta-FAK feedback loop in HTR8SVneo cells.

Roseweir AK, Katz AA, Millar RP

Placenta18 Feb 2012Cited by 41 (Europe PMC)

PMID22349353 (opens in a new tab)DOI10.1016/j.placenta.2012.02.001 (opens in a new tab)PepBankPUB-22349353
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

8 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In HTR8SVneo placental cells, 125I-kisspeptin‑10 bound specifically to the cells.

    In plain wordsIn cells from the human placenta, labeled kisspeptin‑10 bound specifically to the cells.

    Peptide
    Kisspeptin-10
    Outcome
    Not applicableBinding of 125I-kisspeptin-10
    Study

    humann: not reported in source

    HTR8SVneo human first trimester extravillous trophoblast cell line
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HTR8SVneo cells specifically bound 125I-Kisspeptin-10
    1 context sentence
    Summary · context
    We therefore assessed the effect of kisspeptin-10 on the in vitro migration of a human placental cell line derived from first trimester extravillious trophoblasts (HTR8SVneo).
  2. 02LabIn vitro (cells)Activity measurement

    In HTR8SVneo placental cells, kisspeptin‑10 did not induce inositol phosphate production.

    In plain wordsIn cells from the human placenta, kisspeptin‑10 did not trigger inositol phosphate production.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeInositol phosphate production
    Study

    humann: not reported in source

    HTR8SVneo human first trimester extravillous trophoblast cell line
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    but kisspeptin-10 did not induce inositol phosphate production.
    1 context sentence
    Summary · context
    We therefore assessed the effect of kisspeptin-10 on the in vitro migration of a human placental cell line derived from first trimester extravillious trophoblasts (HTR8SVneo).
  3. 03LabIn vitro (cells)Efficacy outcome

    In HTR8SVneo placental cells, kisspeptin‑10 inhibited cell migration, with maximal inhibition at 100 nM.

    In plain wordsIn cells from the human placenta, kisspeptin‑10 slowed cell movement, most strongly at 100 nM.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseCell migration
    Study

    humann: not reported in source

    HTR8SVneo human first trimester extravillous trophoblast cell line
    Outcome group
    Cancer cell invasion and metastasis · Reproductive and sexual health
    Studied dose
    100 nMAs given in this study (human).

    Measurements

    Concentration of maximal inhibition
    100 nM
    cell migration

    Source sentences

    Summary
    Cell migration was inhibited by kisspeptin-10 with a maximal inhibition at 100nM.
    1 context sentence
    Summary · context
    We therefore assessed the effect of kisspeptin-10 on the in vitro migration of a human placental cell line derived from first trimester extravillious trophoblasts (HTR8SVneo).
  4. 04LabIn vitro (cells)Mechanism

    In HTR8SVneo placental cells, kisspeptin‑10 increased phosphorylation of GSK3 beta at Ser9 3-fold at 5 min.

    In plain wordsIn cells from the human placenta, kisspeptin‑10 increased a chemical tag on the GSK3 beta enzyme 3-fold after 5 minutes.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseGSK3 beta Ser9 phosphorylation
    Study

    humann: not reported in source

    HTR8SVneo human first trimester extravillous trophoblast cell line
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · 5 minAs given in this study (human).

    Measurements

    Fold increase
    3 fold
    GSK3 beta Ser9 phosphorylation at 5 min

    Source sentences

    Summary
    Treatment with kisspeptin-10 elicited phosphorylation of GSK3 beta at Ser9 (which inhibits activity), with a 3-fold increase at 5 min.
    1 context sentence
    Summary · context
    We therefore assessed the effect of kisspeptin-10 on the in vitro migration of a human placental cell line derived from first trimester extravillious trophoblasts (HTR8SVneo).
  5. 05LabIn vitro (cells)Mechanism

    In HTR8SVneo placental cells, kisspeptin‑10 caused transient phosphorylation of ERK1/2 and p38MAPK that peaked at 10 min.

    In plain wordsIn cells from the human placenta, kisspeptin‑10 briefly raised chemical tags on ERK1/2 and p38MAPK, peaking at 10 minutes.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseERK1/2 and p38MAPK phosphorylation
    Study

    humann: not reported in source

    HTR8SVneo human first trimester extravillous trophoblast cell line
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · 10 minAs given in this study (human).

    Source sentences

    Summary
    Transient phosphorylation of ERK1/2 and p38MAPK peaked at 10min.
    1 context sentence
    Summary · context
    We therefore assessed the effect of kisspeptin-10 on the in vitro migration of a human placental cell line derived from first trimester extravillious trophoblasts (HTR8SVneo).
  6. 06LabIn vitro (cells)Mechanism

    In HTR8SVneo placental cells, kisspeptin‑10 increased phosphorylation of focal adhesion kinase at Tyr925 3-fold at 10 min.

    In plain wordsIn cells from the human placenta, kisspeptin‑10 increased a chemical tag on focal adhesion kinase 3-fold after 10 minutes.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseFAK Tyr925 phosphorylation
    Study

    humann: not reported in source

    HTR8SVneo human first trimester extravillous trophoblast cell line
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated · 10 minAs given in this study (human).

    Measurements

    Fold increase
    3 fold
    FAK Tyr925 phosphorylation at 10 min

    Source sentences

    Summary
    Phosphorylation of focal adhesion kinase (FAK) at Tyr925 increased 3-fold at 10 min.
    1 context sentence
    Summary · context
    We therefore assessed the effect of kisspeptin-10 on the in vitro migration of a human placental cell line derived from first trimester extravillious trophoblasts (HTR8SVneo).
  7. 07LabIn vitro (cells)Mechanism

    In HTR8SVneo placental cells, kisspeptin‑10 inhibition of GSK3 beta correlated with release of beta-catenin into the cytoplasm.

    In plain wordsIn cells from the human placenta, kisspeptin‑10 action on GSK3 beta was linked to beta-catenin moving into the cell fluid.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseBeta-catenin release into the cytoplasm
    Study

    humann: not reported in source

    HTR8SVneo human first trimester extravillous trophoblast cell line
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Inhibition of GSK3 beta correlated with release of beta-catenin into the cytoplasm.
    1 context sentence
    Summary · context
    We therefore assessed the effect of kisspeptin-10 on the in vitro migration of a human placental cell line derived from first trimester extravillious trophoblasts (HTR8SVneo).
  8. 08LabIn vitro (cells)Mechanism

    In HTR8SVneo placental cells, inhibitors of G(q/11), Src, EGFR, PI(3)K, PKC and MEK differentially blocked the signaling events caused by kisspeptin‑10.

    In plain wordsIn cells from the human placenta, drugs that block several signaling proteins blocked the signaling steps that kisspeptin‑10 triggered.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKisspeptin-10 signaling events blocked by inhibitors
    Study

    humann: not reported in source

    HTR8SVneo human first trimester extravillous trophoblast cell line
    Outcome group
    Receptor activity and mechanism · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    These signaling events were differentially blocked by inhibitors of G(q/11), Src, EGFR, PI(3)K, PKC and MEK.
    1 context sentence
    Summary · context
    We therefore assessed the effect of kisspeptin-10 on the in vitro migration of a human placental cell line derived from first trimester extravillious trophoblasts (HTR8SVneo).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

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