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Study · Ex vivo tissue · mouse · 2012

11 results

Kisspeptin inhibits high-voltage activated Ca2+ channels in GnRH neurons via multiple Ca2+ influx and release pathways.

Zhang XB, Spergel DJ

Neuroendocrinology14 Feb 2012Cited by 27 (Europe PMC)

PMID22343183 (opens in a new tab)DOI10.1159/000335985 (opens in a new tab)PepBankPUB-22343183
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

11 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, prolonged KP-10 application inhibited Ca2+ currents.

    In plain wordsIn lab tests on mouse brain slices, kisspeptin‑10 reduced calcium currents in GnRH nerve cells.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseHigh-voltage activated Ca2+ currents
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated · >30 sAs given in this study (mouse).

    Source sentences

    Summary
    Prolonged (>30 s) KP-10 application inhibited Ca(2+) currents.
    1 context sentence
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.
  2. 02LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, the GPR54 antagonist peptide 234 prevented KP-10 inhibition of Ca2+ currents.

    In plain wordsIn lab tests on mouse brain slices, a GPR54 blocker stopped kisspeptin‑10 from reducing calcium currents.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKP-10 inhibition with GPR54 antagonist peptide 234
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The GPR54 antagonist peptide 234, chelation of intracellular Ca(2+) by 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid, substitution of Ba(2+) for Ca(2+)
    2 context sentences
    Summary · context
    cyclopiazonic acid each prevented inhibition.
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.
  3. 03LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, chelating intracellular Ca2+ prevented KP-10 inhibition of Ca2+ currents.

    In plain wordsIn lab tests on mouse brain slices, removing calcium inside the cell stopped kisspeptin‑10 from reducing calcium currents.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKP-10 inhibition with intracellular Ca2+ chelation
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The GPR54 antagonist peptide 234, chelation of intracellular Ca(2+) by 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid, substitution of Ba(2+) for Ca(2+)
    2 context sentences
    Summary · context
    cyclopiazonic acid each prevented inhibition.
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.
  4. 04LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, substituting Ba2+ for Ca2+ prevented KP-10 inhibition of the currents.

    In plain wordsIn lab tests on mouse brain slices, swapping barium for calcium stopped kisspeptin‑10 from reducing the currents.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKP-10 inhibition with Ba2+ substituted for Ca2+
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The GPR54 antagonist peptide 234, chelation of intracellular Ca(2+) by 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid, substitution of Ba(2+) for Ca(2+)
    2 context sentences
    Summary · context
    cyclopiazonic acid each prevented inhibition.
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.
  5. 05LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, the calmodulin antagonists calmidazolium and trifluoperazine prevented KP-10 inhibition of Ca2+ currents.

    In plain wordsIn lab tests on mouse brain slices, calmodulin blockers stopped kisspeptin‑10 from reducing calcium currents.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKP-10 inhibition with calmodulin antagonists
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    the calmodulin antagonists calmidazolium and trifluoperazine, the phospholipase C inhibitor edelfosine, the canonical transient receptor potential (TRPC) channel and inositol 1,4,5-trisphosphate receptor (IP(3)R) antagonist 2-APB, the TRPC channel antagonist BTP2 and the endoplasmic reticulum Ca(2+)…
    1 context sentence
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.
  6. 06LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, the phospholipase C inhibitor edelfosine prevented KP-10 inhibition of Ca2+ currents.

    In plain wordsIn lab tests on mouse brain slices, a phospholipase C blocker stopped kisspeptin‑10 from reducing calcium currents.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKP-10 inhibition with phospholipase C inhibitor
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    the calmodulin antagonists calmidazolium and trifluoperazine, the phospholipase C inhibitor edelfosine, the canonical transient receptor potential (TRPC) channel and inositol 1,4,5-trisphosphate receptor (IP(3)R) antagonist 2-APB, the TRPC channel antagonist BTP2 and the endoplasmic reticulum Ca(2+)…
    1 context sentence
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.
  7. 07LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, the TRPC channel and IP3 receptor antagonist 2-APB prevented KP-10 inhibition of Ca2+ currents.

    In plain wordsIn lab tests on mouse brain slices, a drug that blocks two calcium pathways stopped kisspeptin‑10 from reducing calcium currents.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKP-10 inhibition with TRPC and IP3R antagonist 2-APB
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    the calmodulin antagonists calmidazolium and trifluoperazine, the phospholipase C inhibitor edelfosine, the canonical transient receptor potential (TRPC) channel and inositol 1,4,5-trisphosphate receptor (IP(3)R) antagonist 2-APB, the TRPC channel antagonist BTP2 and the endoplasmic reticulum Ca(2+)…
    1 context sentence
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.
  8. 08LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, the TRPC channel antagonist BTP2 prevented KP-10 inhibition of Ca2+ currents.

    In plain wordsIn lab tests on mouse brain slices, a TRPC channel blocker stopped kisspeptin‑10 from reducing calcium currents.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKP-10 inhibition with TRPC channel antagonist BTP2
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    the calmodulin antagonists calmidazolium and trifluoperazine, the phospholipase C inhibitor edelfosine, the canonical transient receptor potential (TRPC) channel and inositol 1,4,5-trisphosphate receptor (IP(3)R) antagonist 2-APB, the TRPC channel antagonist BTP2 and the endoplasmic reticulum Ca(2+)…
    1 context sentence
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.
  9. 09LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, the endoplasmic reticulum Ca2+-ATPase blocker cyclopiazonic acid prevented KP-10 inhibition of Ca2+ currents.

    In plain wordsIn lab tests on mouse brain slices, a blocker of the calcium pump in the cell store stopped kisspeptin‑10 from reducing calcium currents.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKP-10 inhibition with endoplasmic reticulum Ca2+-ATPase blocker
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    the calmodulin antagonists calmidazolium and trifluoperazine, the phospholipase C inhibitor edelfosine, the canonical transient receptor potential (TRPC) channel and inositol 1,4,5-trisphosphate receptor (IP(3)R) antagonist 2-APB, the TRPC channel antagonist BTP2 and the endoplasmic reticulum Ca(2+)…
    1 context sentence
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.
  10. 10LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, the IP3 receptor antagonists caffeine, heparin and intracellular 2-APB prevented KP-10 inhibition to a lesser extent.

    In plain wordsIn lab tests on mouse brain slices, IP3 receptor blockers only partly stopped kisspeptin‑10 from reducing calcium currents.

    Peptide
    Kisspeptin-10
    Outcome
    MixedKP-10 inhibition with IP3 receptor antagonists
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The IP(3)R antagonists caffeine (10 µM), heparin and intracellular 2-APB prevented inhibition to a lesser extent.
    1 context sentence
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.
  11. 11LabEx vivo tissueMechanism

    In GnRH neurons in mouse brain slices, the ryanodine receptor antagonists ryanodine and dantrolene prevented KP-10 inhibition of Ca2+ currents.

    In plain wordsIn lab tests on mouse brain slices, ryanodine receptor blockers stopped kisspeptin‑10 from reducing calcium currents.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKP-10 inhibition with ryanodine receptor antagonists
    Study

    mousen: not reported in source

    GnRH neurons in brain slices from pubertal male GnRH-GFP transgenic mice
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The ryanodine receptor (RyR) antagonists ryanodine and dantrolene prevented inhibition
    1 context sentence
    Summary · context
    We used whole-cell patch-clamp recording to examine the effects of kisspeptin-10 (KP-10) on VGCC activity evoked by step depolarizations in GnRH neurons in brain slices from pubertal male GnRH-green fluorescent protein transgenic mice.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

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