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Study · In vitro (cells) · 2012

7 results

MC1R variant allele effects on UVR-induced phosphorylation of p38, p53, and DDB2 repair protein responses in melanocytic cells in culture.

Wong SS, Ainger SA, Leonard JH, Sturm RA

The Journal of investigative dermatology16 Feb 2012Cited by 31 (Europe PMC)

PMID22336944 (opens in a new tab)DOI10.1038/jid.2011.473 (opens in a new tab)PepBankPUB-22336944
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In cocultures with MC1R wild-type melanocytes, NDP-MSH with UVR gave synergistic activation of p38 phosphorylation.

    In plain wordsIn lab cultures of skin cells with normal MC1R, NDP-MSH and UV light together strongly raised p38 activation.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Increasep38 phosphorylation in MC1R wild-type melanocyte cocultures
    Study

    humann: not reported in source

    Melanocyte-keratinocyte cocultures with UVR exposure
    Outcome group
    Receptor activity and mechanism · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Wild-type MC1R melanocyte strains grown together with keratinocytes in coculture, when treated with NDP-MSH and exposed to UVR, gave synergistic activation of p38 and p53 phosphorylation, and were not replicated by R/R variant melanocytes, which have lower basal levels of phosphorylated forms of p38.
    1 context sentence
    Summary · context
    We report the effects of MC1R ligand treatment and UVR exposure on phosphorylation of p38 and p53, and DDB2 protein expression in MC1R variant strains.
  2. 02LabIn vitro (cells)Mechanism

    In cocultures with MC1R wild-type melanocytes, NDP-MSH with UVR gave synergistic activation of p53 phosphorylation.

    In plain wordsIn lab cultures of skin cells with normal MC1R, NDP-MSH and UV light together strongly raised p53 activation.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Increasep53 phosphorylation in MC1R wild-type melanocyte cocultures
    Study

    humann: not reported in source

    Melanocyte-keratinocyte cocultures with UVR exposure
    Outcome group
    Receptor activity and mechanism · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Wild-type MC1R melanocyte strains grown together with keratinocytes in coculture, when treated with NDP-MSH and exposed to UVR, gave synergistic activation of p38 and p53 phosphorylation, and were not replicated by R/R variant melanocytes, which have lower basal levels of phosphorylated forms of p38.
    1 context sentence
    Summary · context
    We report the effects of MC1R ligand treatment and UVR exposure on phosphorylation of p38 and p53, and DDB2 protein expression in MC1R variant strains.
  3. 03LabIn vitro (cells)Mechanism

    In cocultures with MC1R R/R variant melanocytes, the synergistic activation of p38 phosphorylation by NDP-MSH with UVR was not replicated.

    In plain wordsIn lab cultures of skin cells with the R/R MC1R variant, NDP-MSH and UV light together did not repeat the strong p38 rise.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changep38 phosphorylation in MC1R R/R variant melanocyte cocultures
    Study

    humann: not reported in source

    Melanocyte-keratinocyte cocultures with UVR exposure
    Outcome group
    Receptor activity and mechanism · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Wild-type MC1R melanocyte strains grown together with keratinocytes in coculture, when treated with NDP-MSH and exposed to UVR, gave synergistic activation of p38 and p53 phosphorylation, and were not replicated by R/R variant melanocytes, which have lower basal levels of phosphorylated forms of p38.
    1 context sentence
    Summary · context
    We report the effects of MC1R ligand treatment and UVR exposure on phosphorylation of p38 and p53, and DDB2 protein expression in MC1R variant strains.
  4. 04LabIn vitro (cells)Mechanism

    In cocultures with MC1R R/R variant melanocytes, the synergistic activation of p53 phosphorylation by NDP-MSH with UVR was not replicated.

    In plain wordsIn lab cultures of skin cells with the R/R MC1R variant, NDP-MSH and UV light together did not repeat the strong p53 rise.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changep53 phosphorylation in MC1R R/R variant melanocyte cocultures
    Study

    humann: not reported in source

    Melanocyte-keratinocyte cocultures with UVR exposure
    Outcome group
    Receptor activity and mechanism · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Wild-type MC1R melanocyte strains grown together with keratinocytes in coculture, when treated with NDP-MSH and exposed to UVR, gave synergistic activation of p38 and p53 phosphorylation, and were not replicated by R/R variant melanocytes, which have lower basal levels of phosphorylated forms of p38.
    1 context sentence
    Summary · context
    We report the effects of MC1R ligand treatment and UVR exposure on phosphorylation of p38 and p53, and DDB2 protein expression in MC1R variant strains.
  5. 05LabIn vitro (cells)Mechanism

    In R/R variant melanocyte cocultures, the minor increases in p38 phosphorylation could be attributed to the keratinocytes alone.

    In plain wordsIn lab cultures of skin cells with the R/R MC1R variant, small p38 increases could be due to the keratinocytes alone.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changep38 increase in R/R variant cocultures
    Study

    humann: not reported in source

    Melanocyte-keratinocyte cocultures with UVR exposure
    Outcome group
    Receptor activity and mechanism · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Minor increases in p38 phosphorylation status in R/R variant melanocyte cocultures could be attributed to the keratinocytes alone.
    1 context sentence
    Summary · context
    We report the effects of MC1R ligand treatment and UVR exposure on phosphorylation of p38 and p53, and DDB2 protein expression in MC1R variant strains.
  6. 06LabIn vitro (cells)Mechanism

    In MC1R wild-type melanocyte strains, DDB2 protein levels were regulated through p38.

    In plain wordsIn lab cultures of skin cells with normal MC1R, DDB2 repair protein levels were controlled through p38.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableDDB2 protein regulation through p38 in MC1R wild-type strains
    Study

    humann: not reported in source

    Melanocyte-keratinocyte cocultures with UVR exposure
    Outcome group
    Receptor activity and mechanism · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We also found that MC1R wild-type strains regulate DDB2 protein levels through p38, but MC1R R/R variant melanocytes do not.
    1 context sentence
    Summary · context
    We report the effects of MC1R ligand treatment and UVR exposure on phosphorylation of p38 and p53, and DDB2 protein expression in MC1R variant strains.
  7. 07LabIn vitro (cells)Mechanism

    In MC1R R/R variant melanocytes, DDB2 protein levels were not regulated through p38.

    In plain wordsIn lab cultures of skin cells with the R/R MC1R variant, DDB2 repair protein levels were not controlled through p38.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeDDB2 protein regulation through p38 in MC1R R/R variant melanocytes
    Study

    humann: not reported in source

    Melanocyte-keratinocyte cocultures with UVR exposure
    Outcome group
    Receptor activity and mechanism · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We also found that MC1R wild-type strains regulate DDB2 protein levels through p38, but MC1R R/R variant melanocytes do not.
    1 context sentence
    Summary · context
    We report the effects of MC1R ligand treatment and UVR exposure on phosphorylation of p38 and p53, and DDB2 protein expression in MC1R variant strains.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

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