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Study · In vitro (cells) · rat · 2012

6 results

Melanocortin 4 receptor activation induces brain-derived neurotrophic factor expression in rat astrocytes through cyclic AMP-protein kinase A pathway.

Caruso C, Carniglia L, Durand D, Gonzalez PV, Scimonelli TN, Lasaga M

Molecular and cellular endocrinology22 Jul 2011Cited by 58 (Europe PMC)

PMID21803120 (opens in a new tab)DOI10.1016/j.mce.2011.07.036 (opens in a new tab)PepBankPUB-21803120
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, NDP-MSH induced production of cAMP.

    In plain wordsIn rat brain cells in a dish, NDP-MSH raised cAMP, a cell signal.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreasecAMP production in astrocytes
    Study

    ratn: not reported in source

    Cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    α-MSH and its analogue NDP-MSH, induced production of cAMP in astrocytes.
    1 context sentence
    Summary · context
    Since brain-derived neurotrophic factor (BDNF) may be mediating MC4R hypothalamic anorexigenic actions, we determined melanocortin effects on BDNF expression in rat cultured astrocytes and certain mechanisms involved in MC4R signaling.
  2. 02LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, the MC4R antagonist HS024 completely blocked the cAMP induction by NDP-MSH.

    In plain wordsIn rat brain cells in a dish, blocking the MC4R receptor with HS024 completely stopped the cAMP rise caused by NDP-MSH.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableEffect of MC4R antagonist HS024 on NDP-MSH cAMP induction
    Study

    ratn: not reported in source

    Cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    α-MSH and its analogue NDP-MSH, induced production of cAMP in astrocytes. This effect was completely blocked by the MC4R antagonist, HS024.
    1 context sentence
    Summary · context
    Since brain-derived neurotrophic factor (BDNF) may be mediating MC4R hypothalamic anorexigenic actions, we determined melanocortin effects on BDNF expression in rat cultured astrocytes and certain mechanisms involved in MC4R signaling.
  3. 03LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, NDP-MSH increased BDNF mRNA levels.

    In plain wordsIn rat brain cells in a dish, NDP-MSH raised BDNF mRNA, a growth factor message.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseBDNF mRNA level
    Study

    ratn: not reported in source

    Cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We found that NDP-MSH increased BDNF mRNA and protein levels in astrocytes.
    1 context sentence
    Summary · context
    Since brain-derived neurotrophic factor (BDNF) may be mediating MC4R hypothalamic anorexigenic actions, we determined melanocortin effects on BDNF expression in rat cultured astrocytes and certain mechanisms involved in MC4R signaling.
  4. 04LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, NDP-MSH increased BDNF protein levels.

    In plain wordsIn rat brain cells in a dish, NDP-MSH raised BDNF protein.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseBDNF protein level
    Study

    ratn: not reported in source

    Cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We found that NDP-MSH increased BDNF mRNA and protein levels in astrocytes.
    1 context sentence
    Summary · context
    Since brain-derived neurotrophic factor (BDNF) may be mediating MC4R hypothalamic anorexigenic actions, we determined melanocortin effects on BDNF expression in rat cultured astrocytes and certain mechanisms involved in MC4R signaling.
  5. 05LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, the adenylate cyclase inhibitor SQ22536 abolished the effect of NDP-MSH on BDNF expression.

    In plain wordsIn rat brain cells in a dish, blocking adenylate cyclase with SQ22536 removed the NDP-MSH effect on BDNF.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableEffect of adenylate cyclase inhibitor SQ22536 on NDP-MSH BDNF induction
    Study

    ratn: not reported in source

    Cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The effect of NDP-MSH on BDNF expression was abolished by the adenylate cyclase inhibitor SQ22536
    1 context sentence
    Summary · context
    Since brain-derived neurotrophic factor (BDNF) may be mediating MC4R hypothalamic anorexigenic actions, we determined melanocortin effects on BDNF expression in rat cultured astrocytes and certain mechanisms involved in MC4R signaling.
  6. 06LabIn vitro (cells)Mechanism

    In cultured rat astrocytes, the PKA inhibitor Rp-cAMP decreased the effect of NDP-MSH on BDNF expression.

    In plain wordsIn rat brain cells in a dish, blocking PKA with Rp-cAMP reduced the NDP-MSH effect on BDNF.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableEffect of PKA inhibitor Rp-cAMP on NDP-MSH BDNF induction
    Study

    ratn: not reported in source

    Cultured rat astrocytes
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The effect of NDP-MSH on BDNF expression was abolished by the adenylate cyclase inhibitor SQ22536, and decreased by the PKA inhibitor Rp-cAMP.
    1 context sentence
    Summary · context
    Since brain-derived neurotrophic factor (BDNF) may be mediating MC4R hypothalamic anorexigenic actions, we determined melanocortin effects on BDNF expression in rat cultured astrocytes and certain mechanisms involved in MC4R signaling.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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