Nr4a1 siRNA expression attenuates α-MSH regulated gene expression in 3T3-L1 adipocytes.

Wang SC, Myers SA, Eriksson NA, Fitzsimmons RL, Muscat GE

Molecular endocrinology (Baltimore, Md.)14 Jan 2011Cited by 19 (Europe PMC)

PMID21239615PMCIDPMC5417310DOI10.1210/me.2010-0231PepBankPUB-21239615

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study18

11 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Mechanism
In male C57BL/6J mice, intraperitoneal NDP-MSH significantly induced Nur77 mRNA in inguinal adipose tissue.
In plain wordsIn male mice, NDP-MSH injected into the belly cavity significantly raised Nur77 gene activity in fat tissue.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreaseNur77 mRNA in adipose tissue
Study
mousen: not reported in source
C57 BL/6J male mice, inguinal adipose tissue
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
In vivo, ip administration of NDP-MSH to C57 BL/6J (male) mice significantly induced the expression of the mRNA encoding Nur77 and increased IL-6, Cox2, Pck1, and Pdk4 mRNA expression in (inguinal) adipose tissue.
Report a problemFND-2123961514
02AnimalAnimal study (in vivo)Mechanism
In male C57BL/6J mice, intraperitoneal NDP-MSH increased IL-6 mRNA expression in inguinal adipose tissue.
In plain wordsIn male mice, NDP-MSH injected into the belly cavity raised IL-6 gene activity in fat tissue.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreaseIL-6 mRNA in adipose tissue
Study
mousen: not reported in source
C57 BL/6J male mice, inguinal adipose tissue
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
In vivo, ip administration of NDP-MSH to C57 BL/6J (male) mice significantly induced the expression of the mRNA encoding Nur77 and increased IL-6, Cox2, Pck1, and Pdk4 mRNA expression in (inguinal) adipose tissue.
Report a problemFND-2123961515
03AnimalAnimal study (in vivo)Mechanism
In male C57BL/6J mice, intraperitoneal NDP-MSH increased Cox2 mRNA expression in inguinal adipose tissue.
In plain wordsIn male mice, NDP-MSH injected into the belly cavity raised Cox2 gene activity in fat tissue.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreaseCox2 mRNA in adipose tissue
Study
mousen: not reported in source
C57 BL/6J male mice, inguinal adipose tissue
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
In vivo, ip administration of NDP-MSH to C57 BL/6J (male) mice significantly induced the expression of the mRNA encoding Nur77 and increased IL-6, Cox2, Pck1, and Pdk4 mRNA expression in (inguinal) adipose tissue.
Report a problemFND-2123961516
04AnimalAnimal study (in vivo)Mechanism
In male C57BL/6J mice, intraperitoneal NDP-MSH increased Pck1 mRNA expression in inguinal adipose tissue.
In plain wordsIn male mice, NDP-MSH injected into the belly cavity raised Pck1 gene activity in fat tissue.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreasePck1 mRNA in adipose tissue
Study
mousen: not reported in source
C57 BL/6J male mice, inguinal adipose tissue
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
In vivo, ip administration of NDP-MSH to C57 BL/6J (male) mice significantly induced the expression of the mRNA encoding Nur77 and increased IL-6, Cox2, Pck1, and Pdk4 mRNA expression in (inguinal) adipose tissue.
Report a problemFND-2123961517
05AnimalAnimal study (in vivo)Mechanism
In male C57BL/6J mice, intraperitoneal NDP-MSH increased Pdk4 mRNA expression in inguinal adipose tissue.
In plain wordsIn male mice, NDP-MSH injected into the belly cavity raised Pdk4 gene activity in fat tissue.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreasePdk4 mRNA in adipose tissue
Study
mousen: not reported in source
C57 BL/6J male mice, inguinal adipose tissue
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated · intraperitonealAs given in this study (mouse).
Source sentences
Summary
In vivo, ip administration of NDP-MSH to C57 BL/6J (male) mice significantly induced the expression of the mRNA encoding Nur77 and increased IL-6, Cox2, Pck1, and Pdk4 mRNA expression in (inguinal) adipose tissue.
Report a problemFND-2123961518
06LabIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, NDP-MSH preferentially induced expression of the NR4A subgroup of nuclear receptors, but not other nuclear receptor superfamily members.
In plain wordsIn mouse fat cells in a dish, NDP-MSH raised the activity of NR4A genes but not of other nuclear receptor genes.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreaseExpression of NR4A subgroup nuclear receptors
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
We have shown that NDP-MSH strikingly and preferentially induces the expression of the NR4A subgroup (but not any other members of the NR superfamily) in differentiated 3T3-L1 adipocytes.
Report a problemFND-2123961501
07LabIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, NDP-MSH treatment induced Il-6 mRNA.
In plain wordsIn mouse fat cells in a dish, NDP-MSH raised Il-6 gene activity.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreaseIl-6 mRNA induction
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Utilization of quantitative PCR on custom-designed metabolic TaqMan low-density arrays identified the concomitant and marked induction of the mRNAs encoding Il-6, Cox2, Pdk4, and Pck-1 after NDP-MSH treatment.
Report a problemFND-2123961502
08LabIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, NDP-MSH treatment induced Cox2 mRNA.
In plain wordsIn mouse fat cells in a dish, NDP-MSH raised Cox2 gene activity.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreaseCox2 mRNA induction
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Utilization of quantitative PCR on custom-designed metabolic TaqMan low-density arrays identified the concomitant and marked induction of the mRNAs encoding Il-6, Cox2, Pdk4, and Pck-1 after NDP-MSH treatment.
Report a problemFND-2123961503
09LabIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, NDP-MSH treatment induced Pdk4 mRNA.
In plain wordsIn mouse fat cells in a dish, NDP-MSH raised Pdk4 gene activity.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreasePdk4 mRNA induction
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Utilization of quantitative PCR on custom-designed metabolic TaqMan low-density arrays identified the concomitant and marked induction of the mRNAs encoding Il-6, Cox2, Pdk4, and Pck-1 after NDP-MSH treatment.
Report a problemFND-2123961504
10LabIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, NDP-MSH treatment induced Pck-1 mRNA.
In plain wordsIn mouse fat cells in a dish, NDP-MSH raised Pck-1 gene activity.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreasePck-1 mRNA induction
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Utilization of quantitative PCR on custom-designed metabolic TaqMan low-density arrays identified the concomitant and marked induction of the mRNAs encoding Il-6, Cox2, Pdk4, and Pck-1 after NDP-MSH treatment.
Report a problemFND-2123961505
11LabIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, the mRNA expression profiles induced by cAMP and NDP-MSH had unique but also overlapping properties.
In plain wordsIn mouse fat cells in a dish, cAMP and NDP-MSH changed some of the same genes and some different genes.
Peptide
Melanotan I (afamelanotide)
Outcome
MixedGene expression profile of cAMP versus NDP-MSH
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
Dose not stated
Source sentences
Summary
Similar experiments demonstrated that the mRNA expression profile induced by cAMP and NDP-MSH treatment displayed unique but also overlapping properties and suggested that melanocortin-mediated induction of gene expression involves cAMP-dependent and -independent signaling.
Report a problemFND-2123961506

Studies that did not test the peptide alone7

In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan I (afamelanotide).

12LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, Nr4a1/Nur77 siRNA suppressed the NDP-MSH-mediated induction of Nr4a1/Nur77.
In plain wordsIn mouse fat cells in a dish, switching off Nur77 with siRNA reduced the NDP-MSH rise in Nur77.
Peptide
Melanotan I (afamelanotide)
Outcome
DecreaseNDP-MSH induction of Nr4a1/Nur77 with Nur77 siRNA
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Source sentences
Summary
Nr4a1/Nur77 small interfering RNA (siRNA) expression suppressed NDP-MSH-mediated induction of Nr4a1/Nur77 and Nr4a3/Nor-1
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).
Report a problemFND-2123961507
13LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, Nr4a1/Nur77 siRNA suppressed the NDP-MSH-mediated induction of Nr4a3/Nor-1.
In plain wordsIn mouse fat cells in a dish, switching off Nur77 with siRNA reduced the NDP-MSH rise in Nor-1.
Peptide
Melanotan I (afamelanotide)
Outcome
DecreaseNDP-MSH induction of Nr4a3/Nor-1 with Nur77 siRNA
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Source sentences
Summary
Nr4a1/Nur77 small interfering RNA (siRNA) expression suppressed NDP-MSH-mediated induction of Nr4a1/Nur77 and Nr4a3/Nor-1
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).
Report a problemFND-2123961508
14LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, Nr4a1/Nur77 siRNA did not suppress the NDP-MSH-mediated induction of Nr4a2/Nurr1.
In plain wordsIn mouse fat cells in a dish, switching off Nur77 with siRNA did not reduce the NDP-MSH rise in Nurr1.
Peptide
Melanotan I (afamelanotide)
Outcome
No significant changeNDP-MSH induction of Nr4a2/Nurr1 with Nur77 siRNA
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Source sentences
Summary
Nr4a1/Nur77 small interfering RNA (siRNA) expression suppressed NDP-MSH-mediated induction of Nr4a1/Nur77 and Nr4a3/Nor-1 (but not Nr4a2/Nurr1).
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).
Report a problemFND-2123961509
15LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, Nur77 siRNA attenuated the NDP-MSH-mediated induction of Il-6 mRNA.
In plain wordsIn mouse fat cells in a dish, switching off Nur77 with siRNA reduced the NDP-MSH rise in Il-6.
Peptide
Melanotan I (afamelanotide)
Outcome
DecreaseNDP-MSH induction of Il-6 mRNA with Nur77 siRNA
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Source sentences
Summary
Moreover, expression of the siRNA-attenuated NDP-MSH mediated induction of the mRNAs encoding Il-6, Cox2/Ptgs2, and Pck-1 expression.
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).
Report a problemFND-2123961510
16LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, Nur77 siRNA attenuated the NDP-MSH-mediated induction of Cox2/Ptgs2 mRNA.
In plain wordsIn mouse fat cells in a dish, switching off Nur77 with siRNA reduced the NDP-MSH rise in Cox2.
Peptide
Melanotan I (afamelanotide)
Outcome
DecreaseNDP-MSH induction of Cox2/Ptgs2 mRNA with Nur77 siRNA
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Source sentences
Summary
Moreover, expression of the siRNA-attenuated NDP-MSH mediated induction of the mRNAs encoding Il-6, Cox2/Ptgs2, and Pck-1 expression.
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).
Report a problemFND-2123961511
17LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism
In differentiated 3T3-L1 adipocytes, Nur77 siRNA attenuated the NDP-MSH-mediated induction of Pck-1 mRNA.
In plain wordsIn mouse fat cells in a dish, switching off Nur77 with siRNA reduced the NDP-MSH rise in Pck-1.
Peptide
Melanotan I (afamelanotide)
Outcome
DecreaseNDP-MSH induction of Pck-1 mRNA with Nur77 siRNA
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Receptor activity and mechanism · Metabolic and weight
Source sentences
Summary
Moreover, expression of the siRNA-attenuated NDP-MSH mediated induction of the mRNAs encoding Il-6, Cox2/Ptgs2, and Pck-1 expression.
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).
Report a problemFND-2123961512
18LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Efficacy outcome
In differentiated 3T3-L1 adipocytes, Nur77 siRNA expression attenuated NDP-MSH-mediated glucose uptake.
In plain wordsIn mouse fat cells in a dish, switching off Nur77 with siRNA reduced the glucose uptake driven by NDP-MSH.
Peptide
Melanotan I (afamelanotide)
Outcome
DecreaseNDP-MSH-mediated glucose uptake with Nur77 siRNA
Study
mousen: not reported in source
Differentiated 3T3-L1 adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
In addition, Nur77 siRNA expression attenuated NDP-MSH-mediated glucose uptake.
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).
Report a problemFND-2123961513

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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Nr4a1 siRNA expression attenuates α-MSH regulated gene expression in 3T3-L1 adipocytes.

Summary

Results from this study18

Studies that did not test the peptide alone7

Version history

Subjects

Publication types

MeSH terms