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Study · In vitro (cells) · rat · 2011

4 results

Kisspeptin-evoked calcium signals in isolated primary rat gonadotropin- releasing hormone neurones.

Kroll H, Bolsover S, Hsu J, Kim SH, Bouloux PM

Neuroendocrinology8 Nov 2010Cited by 26 (Europe PMC)

PMID21051881 (opens in a new tab)DOI10.1159/000321678 (opens in a new tab)PepBankPUB-21051881
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In isolated primary rat GnRH neurones, 100 nM kisspeptin‑10 produced a maintained increase of cytosolic calcium.

    In plain wordsIn lab-grown rat brain cells, kisspeptin‑10 caused calcium inside the cells to rise and stay high.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseCytosolic calcium in GnRH neurones
    Study

    ratn: not reported in source

    isolated primary rat GnRH neurones, Fura Red calcium imaging
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nMAs given in this study (rat).

    Source sentences

    Summary
    GnRH neurones show a maintained increase of cytosolic calcium upon stimulation with 100 nM kisspeptin-10.
    1 context sentence
    Summary · context
    Primary GnRH neurones were isolated from young adult rats and loaded with the calcium indicator Fura Red.
  2. 02LabIn vitro (cells)Mechanism

    In isolated primary rat GnRH neurones, tetrodotoxin, a sodium channel blocker, reduced the kisspeptin-evoked calcium elevation by 30%.

    In plain wordsIn lab-grown rat brain cells, blocking sodium channels lowered the kisspeptin-driven calcium rise by 30%.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseEffect of tetrodotoxin on kisspeptin-evoked calcium elevation
    Study

    ratn: not reported in source

    isolated primary rat GnRH neurones, Fura Red calcium imaging
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nMAs given in this study (rat).

    Measurements

    Inhibition of calcium elevation
    30%
    1 μM tetrodotoxin

    Source sentences

    Summary
    The calcium elevation was inhibited 30% by 1 μM tetrodotoxin, a voltage-gated sodium channel blocker
    2 context sentences
    Summary · context
    GnRH neurones show a maintained increase of cytosolic calcium upon stimulation with 100 nM kisspeptin-10.
    Summary · context
    Primary GnRH neurones were isolated from young adult rats and loaded with the calcium indicator Fura Red.
  3. 03LabIn vitro (cells)Mechanism

    In isolated primary rat GnRH neurones, SKF96365, an inhibitor of receptor-mediated calcium entry, reduced the kisspeptin-evoked calcium elevation by 76%.

    In plain wordsIn lab-grown rat brain cells, blocking one calcium entry route strongly lowered the kisspeptin-driven calcium rise.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseEffect of SKF96365 on kisspeptin-evoked calcium elevation
    Study

    ratn: not reported in source

    isolated primary rat GnRH neurones, Fura Red calcium imaging
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nMAs given in this study (rat).

    Measurements

    Inhibition of calcium elevation
    76%
    30 μM SKF96365

    Source sentences

    Summary
    and 76% by 30 μM SKF96365, an inhibitor of receptor-mediated calcium entry.
    2 context sentences
    Summary · context
    GnRH neurones show a maintained increase of cytosolic calcium upon stimulation with 100 nM kisspeptin-10.
    Summary · context
    Primary GnRH neurones were isolated from young adult rats and loaded with the calcium indicator Fura Red.
  4. 04LabIn vitro (cells)Mechanism

    In isolated primary rat GnRH neurones, removal of extracellular calcium completely abolished the kisspeptin-induced calcium elevation.

    In plain wordsIn lab-grown rat brain cells, taking calcium out of the surrounding fluid fully stopped the kisspeptin-driven calcium rise.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseEffect of removing extracellular calcium on kisspeptin-evoked calcium elevation
    Study

    ratn: not reported in source

    isolated primary rat GnRH neurones, Fura Red calcium imaging
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nMAs given in this study (rat).

    Source sentences

    Summary
    Furthermore, removal of extracellular calcium completely abolished the kisspeptin-induced calcium elevation.
    2 context sentences
    Summary · context
    GnRH neurones show a maintained increase of cytosolic calcium upon stimulation with 100 nM kisspeptin-10.
    Summary · context
    Primary GnRH neurones were isolated from young adult rats and loaded with the calcium indicator Fura Red.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

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