The role of sexual steroid hormones in the direct stimulation by Kisspeptin-10 of the secretion of luteinizing hormone, follicle-stimulating hormone and prolactin from bovine anterior pituitary cells.

Ezzat AA, Saito H, Sawada T, Yaegashi T, Goto Y, Nakajima Y, Jin J, Yamashita T, Sawai K, Hashizume T

Animal reproduction science11 Jun 2010Cited by 27 (Europe PMC)

PMID20594780DOI10.1016/j.anireprosci.2010.06.002PepBankPUB-20594780

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

8 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In bovine pituitary cells pretreated with estradiol, kisspeptin‑10 significantly stimulated LH secretion.
In plain wordsIn lab-grown calf pituitary cells pretreated with estradiol, kisspeptin‑10 raised LH release.
Peptide
Kisspeptin-10
Outcome
IncreaseLH secretion in estradiol-treated cells
Study
cattlen: not reported in source
Primary bovine anterior pituitary cells from 7-week-old male calves
Comparator
Vehicle-treated control cells
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-6)M · 2hAs given in this study (cattle).
Source sentences
Summary
Kp10 significantly stimulated the secretion of LH from the AP cells treated with E(2) and T (P<0.05)
1 context sentence(design, population, supporting numbers)
Summary · context
AP cells prepared from 7-week-old male calves were incubated for 12h with estradiol (E(2); 10(-8)M), progesterone (P(4); 10(-8)M), testosterone (T; 10(-8)M), or vehicle only (control), and then for 2h with Kp10 (10(-6)M).
Report a problemFND-2059478001
02LabIn vitro (cells)Efficacy outcome
In bovine pituitary cells pretreated with testosterone, kisspeptin‑10 significantly stimulated LH secretion.
In plain wordsIn lab-grown calf pituitary cells pretreated with testosterone, kisspeptin‑10 raised LH release.
Peptide
Kisspeptin-10
Outcome
IncreaseLH secretion in testosterone-treated cells
Study
cattlen: not reported in source
Primary bovine anterior pituitary cells from 7-week-old male calves
Comparator
Vehicle-treated control cells
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-6)M · 2hAs given in this study (cattle).
Source sentences
Summary
Kp10 significantly stimulated the secretion of LH from the AP cells treated with E(2) and T (P<0.05)
1 context sentence(design, population, supporting numbers)
Summary · context
AP cells prepared from 7-week-old male calves were incubated for 12h with estradiol (E(2); 10(-8)M), progesterone (P(4); 10(-8)M), testosterone (T; 10(-8)M), or vehicle only (control), and then for 2h with Kp10 (10(-6)M).
Report a problemFND-2059478002
03LabIn vitro (cells)Efficacy outcome
In bovine pituitary cells pretreated with progesterone, kisspeptin‑10 did not stimulate LH secretion.
In plain wordsIn lab-grown calf pituitary cells pretreated with progesterone, kisspeptin‑10 did not raise LH release.
Peptide
Kisspeptin-10
Outcome
No significant changeLH secretion in progesterone-treated cells
Study
cattlen: not reported in source
Primary bovine anterior pituitary cells from 7-week-old male calves
Comparator
Vehicle-treated control cells
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-6)M · 2hAs given in this study (cattle).
Source sentences
Summary
Kp10 significantly stimulated the secretion of LH from the AP cells treated with E(2) and T (P<0.05), but not from the P(4)-treated cells.
1 context sentence(design, population, supporting numbers)
Summary · context
AP cells prepared from 7-week-old male calves were incubated for 12h with estradiol (E(2); 10(-8)M), progesterone (P(4); 10(-8)M), testosterone (T; 10(-8)M), or vehicle only (control), and then for 2h with Kp10 (10(-6)M).
Report a problemFND-2059478003
04LabIn vitro (cells)Efficacy outcome
In bovine pituitary cells, kisspeptin‑10 had no effect on FSH secretion regardless of the steroid treatment.
In plain wordsIn lab-grown calf pituitary cells, kisspeptin‑10 did not change FSH release, whatever the steroid treatment.
Peptide
Kisspeptin-10
Outcome
No significant changeFSH secretion
Study
cattlen: not reported in source
Primary bovine anterior pituitary cells from 7-week-old male calves
Comparator
Vehicle-treated control cells
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-6)M · 2hAs given in this study (cattle).
Source sentences
Summary
In contrast, Kp10 had no effect on the secretion of FSH regardless of the steroid treatment.
1 context sentence(design, population, supporting numbers)
Summary · context
AP cells prepared from 7-week-old male calves were incubated for 12h with estradiol (E(2); 10(-8)M), progesterone (P(4); 10(-8)M), testosterone (T; 10(-8)M), or vehicle only (control), and then for 2h with Kp10 (10(-6)M).
Report a problemFND-2059478004
05LabIn vitro (cells)Efficacy outcome
In bovine pituitary cells, kisspeptin‑10 significantly stimulated prolactin secretion.
In plain wordsIn lab-grown calf pituitary cells, kisspeptin‑10 raised prolactin release.
Peptide
Kisspeptin-10
Outcome
IncreasePRL secretion
Study
cattlen: not reported in source
Primary bovine anterior pituitary cells from 7-week-old male calves
Comparator
Vehicle-treated control cells
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-6)M · 2hAs given in this study (cattle).
Source sentences
Summary
Kp10 significantly stimulated the secretion of PRL (P<0.05)
1 context sentence(design, population, supporting numbers)
Summary · context
AP cells prepared from 7-week-old male calves were incubated for 12h with estradiol (E(2); 10(-8)M), progesterone (P(4); 10(-8)M), testosterone (T; 10(-8)M), or vehicle only (control), and then for 2h with Kp10 (10(-6)M).
Report a problemFND-2059478005
06LabIn vitro (cells)Efficacy outcome
In bovine pituitary cells, sex steroid pretreatment did not enhance the prolactin response to kisspeptin‑10.
In plain wordsIn lab-grown calf pituitary cells, sex steroids did not strengthen the prolactin response to kisspeptin‑10.
Peptide
Kisspeptin-10
Outcome
No significant changeEffect of sex steroids on kisspeptin-10-stimulated PRL secretion
Study
cattlen: not reported in source
Primary bovine anterior pituitary cells from 7-week-old male calves
Comparator
Cells without steroid pretreatment
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-6)M · 2hAs given in this study (cattle).
Source sentences
Summary
Kp10 significantly stimulated the secretion of PRL (P<0.05), the sexual steroid hormones having no effect.
1 context sentence(design, population, supporting numbers)
Summary · context
AP cells prepared from 7-week-old male calves were incubated for 12h with estradiol (E(2); 10(-8)M), progesterone (P(4); 10(-8)M), testosterone (T; 10(-8)M), or vehicle only (control), and then for 2h with Kp10 (10(-6)M).
Report a problemFND-2059478006
07LabIn vitro (cells)Efficacy outcome
In bovine pituitary cells, the LH and FSH response to kisspeptin‑10 was significantly smaller than the response to GnRH.
In plain wordsIn lab-grown calf pituitary cells, kisspeptin‑10 raised LH and FSH release less than GnRH did.
Peptide
Kisspeptin-10
Outcome
DecreaseLH and FSH response to kisspeptin-10 compared with GnRH
Study
cattlen: not reported in source
Primary bovine anterior pituitary cells from 7-week-old male calves
Comparator
GnRH 10(-8)M
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-6)M · 2hAs given in this study (cattle).
Source sentences
Summary
The LH- or FSH-releasing response to gonadotropin-releasing hormone (GnRH; 10(-8)M) and PRL-releasing response to thyrotropin-releasing hormone (TRH; 10(-8)M) were significantly greater than those to Kp10 (P<0.05)
1 context sentence(design, population, supporting numbers)
Summary · context
AP cells prepared from 7-week-old male calves were incubated for 12h with estradiol (E(2); 10(-8)M), progesterone (P(4); 10(-8)M), testosterone (T; 10(-8)M), or vehicle only (control), and then for 2h with Kp10 (10(-6)M).
Report a problemFND-2059478007
08LabIn vitro (cells)Efficacy outcome
In bovine pituitary cells, the prolactin response to kisspeptin‑10 was significantly smaller than the response to TRH.
In plain wordsIn lab-grown calf pituitary cells, kisspeptin‑10 raised prolactin release less than TRH did.
Peptide
Kisspeptin-10
Outcome
DecreasePRL response to kisspeptin-10 compared with TRH
Study
cattlen: not reported in source
Primary bovine anterior pituitary cells from 7-week-old male calves
Comparator
TRH 10(-8)M
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
10(-6)M · 2hAs given in this study (cattle).
Source sentences
Summary
The LH- or FSH-releasing response to gonadotropin-releasing hormone (GnRH; 10(-8)M) and PRL-releasing response to thyrotropin-releasing hormone (TRH; 10(-8)M) were significantly greater than those to Kp10 (P<0.05)
1 context sentence(design, population, supporting numbers)
Summary · context
AP cells prepared from 7-week-old male calves were incubated for 12h with estradiol (E(2); 10(-8)M), progesterone (P(4); 10(-8)M), testosterone (T; 10(-8)M), or vehicle only (control), and then for 2h with Kp10 (10(-6)M).
Report a problemFND-2059478008

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

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PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
9 Oct 2026, 18:17 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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