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Study · Not a test of the peptide alone · 2010

3 results

Fluorescence anisotropy assay for pharmacological characterization of ligand binding dynamics to melanocortin 4 receptors.

Veiksina S, Kopanchuk S, Rinken A

Analytical biochemistry17 Mar 2010Cited by 27 (Europe PMC)

PMID20302839 (opens in a new tab)DOI10.1016/j.ab.2010.03.022 (opens in a new tab)PepBankPUB-20302839
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan I (afamelanotide).

  1. 01LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cell-free)Activity measurement

    In membranes of Sf9 cells expressing MC(4) receptors, Cy3B-NDP-alpha-MSH and TAMRA-NDP-alpha-MSH both bound in a saturable way and with high affinity.

    In plain wordsIn lab tests, both labeled NDP-alpha-MSH peptides bound to MC4 receptors in a saturable way and with high affinity.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableBinding of fluorophore-labeled NDP-alpha-MSH ligands to MC(4) receptors
    Study

    insect cells (Sf9)n: not reported in source

    Membranes of Sf9 insect cells expressing MC(4) receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The binding of both ligands to membranes of Sf9 cells expressing MC(4) receptors was saturable and with high affinity.
    1 context sentence
    Summary · context
    Two different red-shifted fluorophore-labeled peptide ligands, Cy3B-NDP-alpha-MSH and TAMRA-NDP-alpha-MSH, were used and compared in assays that monitored their binding to MC(4) receptors in membranes of Sf9 insect cells.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  2. 02LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cell-free)Activity measurement

    In MC(4) receptor binding assays, the Cy3B-labeled ligand performed better than the TAMRA-labeled ligand, with higher photostability, insensitivity to buffer properties and better signal/noise ratio.

    In plain wordsIn lab tests, the Cy3B-labeled peptide worked better in the assay than the TAMRA-labeled one.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableAssay performance of Cy3B-labeled versus TAMRA-labeled NDP-alpha-MSH
    Study

    insect cells (Sf9)n: not reported in source

    Membranes of Sf9 insect cells expressing MC(4) receptors
    Comparator
    TAMRA-labeled ligand
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The Cy3B dye-labeled ligand exhibited improved performance in assays when compared with the TAMRA-labeled ligand, having higher photostability, insensitivity to buffer properties, and better signal/noise ratio.
    1 context sentence
    Summary · context
    Two different red-shifted fluorophore-labeled peptide ligands, Cy3B-NDP-alpha-MSH and TAMRA-NDP-alpha-MSH, were used and compared in assays that monitored their binding to MC(4) receptors in membranes of Sf9 insect cells.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  3. 03LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cell-free)Activity measurement

    In real-time monitoring, equilibrium of peptide binding to MC(4) receptors was not reached even after 3 h.

    In plain wordsIn lab tests, the labeled peptide had not finished binding to the MC4 receptors even after 3 hours.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableTime to binding equilibrium
    Study

    insect cells (Sf9)n: not reported in source

    Membranes of Sf9 insect cells expressing MC(4) receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    Time without equilibrium
    > 3 h
    peptide binding to MC(4) receptors

    Source sentences

    Summary
    On-line monitoring of the reactions revealed that equilibrium of peptide binding was not reached even after 3h.
    1 context sentence
    Summary · context
    Two different red-shifted fluorophore-labeled peptide ligands, Cy3B-NDP-alpha-MSH and TAMRA-NDP-alpha-MSH, were used and compared in assays that monitored their binding to MC(4) receptors in membranes of Sf9 insect cells.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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