Molecular characterization and estrogen regulation of hypothalamic KISS1 gene in the pig.

Tomikawa J, Homma T, Tajima S, Shibata T, Inamoto Y, Takase K, Inoue N, Ohkura S, Uenoyama Y, Maeda K, Tsukamura H

Biology of reproduction14 Oct 2009Cited by 90 (Europe PMC)

PMID19828777DOI10.1095/biolreprod.109.079863PepBankPUB-19828777

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Natural levels measured4

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
In the pig hypothalamus, KISS1-positive cells were mainly in the periventricular nucleus and the arcuate nucleus.
In plain wordsIn pigs, cells making the body's own kisspeptin gene were mainly found in two brain areas, the periventricular and arcuate nuclei.
Peptide
Kisspeptin-10
Outcome
Not applicableDistribution of KISS1-positive cells in hypothalamus
Study
pign: not reported in source
Ovariectomized (OVX) pigs
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
In situ hybridization analysis revealed that KISS1-positive cell populations were mainly distributed in the hypothalamic periventricular nucleus (PeN) and arcuate nucleus (ARC).
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we cloned the porcine KISS1 cDNA from the hypothalamic tissue and investigated the effect of estrogen on the distribution and numbers of KISS1 mRNA-expressing cells in the porcine hypothalamus.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-1982877702
02AnimalEndogenous levelAnimal study (in vivo)Mechanism
In ovariectomized pigs, estradiol benzoate treatment upregulated KISS1 expression in the periventricular nucleus.
In plain wordsIn pigs, estrogen raised the body's own kisspeptin gene activity in one brain area compared with pigs without estrogen.
Peptide
Kisspeptin-10
Outcome
IncreaseKISS1 expression in the periventricular nucleus
Study
pign: not reported in source
Ovariectomized (OVX) pigs
Comparator
OVX pigs without estradiol benzoate
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
KISS1 expression in the PeN of ovariectomized (OVX) pigs was significantly upregulated by estradiol benzoate (EB) treatment.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we cloned the porcine KISS1 cDNA from the hypothalamic tissue and investigated the effect of estrogen on the distribution and numbers of KISS1 mRNA-expressing cells in the porcine hypothalamus.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-1982877703
03AnimalEndogenous levelAnimal study (in vivo)Mechanism
In ovariectomized pigs, estradiol benzoate lowered the number of KISS1-expressing neurons in the most caudal part of the arcuate nucleus.
In plain wordsIn pigs, estrogen lowered the number of cells making the body's own kisspeptin gene in the rear part of one brain area.
Peptide
Kisspeptin-10
Outcome
DecreaseNumber of KISS1-expressing neurons in the caudal arcuate nucleus
Study
pign: not reported in source
Ovariectomized (OVX) pigs
Comparator
OVX pigs without estradiol benzoate
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
The number of KISS1-expressing neurons was significantly lowered by EB treatment only in the most caudal part of the ARC
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we cloned the porcine KISS1 cDNA from the hypothalamic tissue and investigated the effect of estrogen on the distribution and numbers of KISS1 mRNA-expressing cells in the porcine hypothalamus.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-1982877704
04AnimalEndogenous levelAnimal study (in vivo)Mechanism
In ovariectomized pigs, estradiol benzoate did not affect KISS1-expressing neurons in the other arcuate nucleus populations.
In plain wordsIn pigs, estrogen did not change the number of cells making the body's own kisspeptin gene in the other parts of that brain area.
Peptide
Kisspeptin-10
Outcome
No significant changeNumber of KISS1-expressing neurons in other arcuate nucleus populations
Study
pign: not reported in source
Ovariectomized (OVX) pigs
Comparator
OVX pigs without estradiol benzoate
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
but other ARC populations were not affected.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we cloned the porcine KISS1 cDNA from the hypothalamic tissue and investigated the effect of estrogen on the distribution and numbers of KISS1 mRNA-expressing cells in the porcine hypothalamus.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-1982877705

Studies that did not test the peptide alone1

In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Kisspeptin-10.

05AnimalNot a test of Kisspeptin-10 aloneAnimal study (in vivo)Other
The cloned porcine KISS1 cDNA was 857 bp and encoded a 54 amino acid kisspeptin whose C-terminal kisspeptin‑10 motif is identical to that of mouse, rat, cattle and sheep.
In plain wordsIn pigs, the kisspeptin gene was cloned; its active kisspeptin‑10 end matches that of mouse, rat, cattle and sheep.
Peptide
Kisspeptin-10
Outcome
Not applicablePorcine KISS1 cDNA cloning
Study
pign: not reported in source
Hypothalamic tissue
Outcome group
Sex hormones and fertility · Pharmacology
Measurements
cDNA length
857 bp
porcine KISS1
Kisspeptin length
54 amino acids
porcine kisspeptin
Source sentences
Summary
The full length of the cDNA was 857 bp encoding the kisspeptin of 54 amino acids, with the C-terminal active motif designated kisspeptin-10 being identical to that of mouse, rat, cattle, and sheep.
1 context sentence(design, population, supporting numbers)
Summary · context
Here, we cloned the porcine KISS1 cDNA from the hypothalamic tissue and investigated the effect of estrogen on the distribution and numbers of KISS1 mRNA-expressing cells in the porcine hypothalamus.
In this study, Kisspeptin-10 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Kisspeptin-10.
Report a problemFND-1982877701

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
9 Oct 2026, 18:17 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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