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Study · Not a test of the peptide alone · 2009

2 results

Observation of NMR signals from proteins introduced into living mammalian cells by reversible membrane permeabilization using a pore-forming toxin, streptolysin O.

Ogino S, Kubo S, Umemoto R, Huang S, Nishida N, Shimada I

Journal of the American Chemical Society1 Aug 2009Cited by 103 (Europe PMC)

PMID19603816 (opens in a new tab)DOI10.1021/ja904407w (opens in a new tab)PepBankPUB-19603816
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone2

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Other

    In 293F cells permeabilized with streptolysin O, in-cell NMR observed 1H-15N HSQC signals from delivered isotope-labeled Tbeta4.

    In plain wordsIn cells, a new NMR method detected signals from labeled Tbeta4 that was placed inside the cells.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableIn-cell NMR signals of Tbeta4
    Study

    humann: not reported in source

    293F cells permeabilized with streptolysin O
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    we successfully observed (1)H-(15)N HSQC signals originating from the Tbeta4
    1 context sentence
    Summary · context
    The stable-isotope-labeled target protein, thymosin beta4 (Tbeta4), was delivered to 293F cells, which were permeabilized by a pore-forming toxin, streptolysin O, and resealed by Ca(2+) after Tbeta4 uptake.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  2. 02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Other

    In-cell NMR showed N-terminal acetylation of Tbeta4, which had occurred inside 293F cells as a post-translational modification.

    In plain wordsIn cells, the NMR method showed that Tbeta4 was chemically modified at its start inside the cell.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableN-terminal acetylation of Tbeta4 inside cells
    Study

    humann: not reported in source

    293F cells permeabilized with streptolysin O
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    including those from the N-terminal acetylation, which had occurred inside the cell as a post-translational modification.
    1 context sentence
    Summary · context
    The stable-isotope-labeled target protein, thymosin beta4 (Tbeta4), was delivered to 293F cells, which were permeabilized by a pore-forming toxin, streptolysin O, and resealed by Ca(2+) after Tbeta4 uptake.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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