Cell-responsive hydrogel for encapsulation of vascular cells.

Kraehenbuehl TP, Ferreira LS, Zammaretti P, Hubbell JA, Langer R

Biomaterials4 Jun 2009Cited by 92 (Europe PMC)

PMID19500842PMCIDPMC4157733DOI10.1016/j.biomaterials.2009.04.057PepBankPUB-19500842

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone6

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome
In human umbilical vein endothelial cells in PEG hydrogels, gels with entrapped Tbeta4 increased cell survival compared with gels without Tbeta4.
In plain wordsIn human vein cells in a gel, a gel holding Tβ4 increased cell survival versus a gel without Tβ4.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseHUVEC survival
Study
humann: not reported in source
HUVEC in MMP-responsive PEG hydrogel
Comparator
Gels without Tbeta4
Outcome group
Cell viability and death · Cardiovascular
Source sentences
Summary
Gels with entrapped Tbeta4 increased the survival of HUVEC compared to gels without Tbeta4, and significantly up-regulated the endothelial genes vascular endothelial-cadherin and angiopoietin-2, whereas von Willebrand factor was significantly down-regulated.
1 context sentence(design, population, supporting numbers)
Summary · context
We show that the physical incorporation of Tbeta4 in this bioactive matrix creates a three-dimensional (3D) environment conducive for human umbilical vein endothelial cell (HUVEC) adhesion, survival, migration and organization.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1950084201
02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In HUVEC in PEG hydrogels, entrapped Tbeta4 significantly up-regulated the endothelial genes vascular endothelial-cadherin and angiopoietin-2.
In plain wordsIn human vein cells in a gel, a gel holding Tβ4 turned up the blood vessel genes VE-cadherin and angiopoietin-2.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseVE-cadherin and angiopoietin-2 gene expression
Study
humann: not reported in source
HUVEC in MMP-responsive PEG hydrogel
Comparator
Gels without Tbeta4
Outcome group
Angiogenesis · Cardiovascular
Source sentences
Summary
Gels with entrapped Tbeta4 increased the survival of HUVEC compared to gels without Tbeta4, and significantly up-regulated the endothelial genes vascular endothelial-cadherin and angiopoietin-2, whereas von Willebrand factor was significantly down-regulated.
1 context sentence(design, population, supporting numbers)
Summary · context
We show that the physical incorporation of Tbeta4 in this bioactive matrix creates a three-dimensional (3D) environment conducive for human umbilical vein endothelial cell (HUVEC) adhesion, survival, migration and organization.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1950084202
03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In HUVEC in PEG hydrogels, entrapped Tbeta4 significantly down-regulated von Willebrand factor.
In plain wordsIn human vein cells in a gel, a gel holding Tβ4 turned down the von Willebrand factor gene.
Peptide
Thymosin beta-4 (human)
Outcome
Decreasevon Willebrand factor gene expression
Study
humann: not reported in source
HUVEC in MMP-responsive PEG hydrogel
Comparator
Gels without Tbeta4
Outcome group
Angiogenesis · Cardiovascular
Source sentences
Summary
Gels with entrapped Tbeta4 increased the survival of HUVEC compared to gels without Tbeta4, and significantly up-regulated the endothelial genes vascular endothelial-cadherin and angiopoietin-2, whereas von Willebrand factor was significantly down-regulated.
1 context sentence(design, population, supporting numbers)
Summary · context
We show that the physical incorporation of Tbeta4 in this bioactive matrix creates a three-dimensional (3D) environment conducive for human umbilical vein endothelial cell (HUVEC) adhesion, survival, migration and organization.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1950084203
04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
Incorporation of Tbeta4 in the hydrogel significantly increased MMP-2 and MMP-9 secretion of encapsulated HUVEC.
In plain wordsA gel holding Tβ4 increased the release of the enzymes MMP-2 and MMP-9 by human vein cells inside it.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseMMP-2 and MMP-9 secretion
Study
humann: not reported in source
HUVEC in MMP-responsive PEG hydrogel
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
Incorporation of Tbeta4 significantly increased MMP-2 and MMP-9 secretion of encapsulated HUVEC.
1 context sentence(design, population, supporting numbers)
Summary · context
We show that the physical incorporation of Tbeta4 in this bioactive matrix creates a three-dimensional (3D) environment conducive for human umbilical vein endothelial cell (HUVEC) adhesion, survival, migration and organization.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1950084204
05LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome
Tbeta4 facilitated HUVEC attachment and induced vascular-like network formation on PEG hydrogels.
In plain wordsA gel holding Tβ4 helped human vein cells attach and form blood-vessel-like networks on it.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseHUVEC attachment and vascular-like network formation
Study
humann: not reported in source
HUVEC in MMP-responsive PEG hydrogel
Outcome group
Angiogenesis · Cardiovascular
Source sentences
Summary
In addition, Tbeta4 facilitated HUVEC attachment and induced vascular-like network formation upon the PEG-hydrogels.
1 context sentence(design, population, supporting numbers)
Summary · context
We show that the physical incorporation of Tbeta4 in this bioactive matrix creates a three-dimensional (3D) environment conducive for human umbilical vein endothelial cell (HUVEC) adhesion, survival, migration and organization.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1950084205
06LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Other
In MMP-responsive PEG hydrogels with encapsulated HUVEC, the gel acted as a controlled Tbeta4-release system, with MMP-2 and MMP-9 enzymes triggering the release.
In plain wordsIn lab tests, the gel released Tbeta4 when the enzymes MMP-2 and MMP-9 acted on it.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableMMP-triggered release of Tbeta4 from the hydrogel
Study
humann: not reported in source
HUVEC in MMP-responsive PEG hydrogel
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
The gel acts as a controlled Tbeta4-release system, as MMP-2 and MMP-9 enzymes trigger the release.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1950084206

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

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