Active form of neuroprotective Humanin, HN, and inactive analog, S7A-HN, are monomeric and disordered in aqueous phosphate solution at pH 6.0; No correlation of solution structure with activity.

Arisaka F, Arakawa T, Niikura T, Kita Y

Protein and peptide letters1 Jan 2009Cited by 6 (Europe PMC)

PMID19200035DOI10.2174/092986609787316225PepBankPUB-19200035

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

01LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism
In aqueous phosphate solution, the parent HN peptide, the inactive analog S7A-HN and the 1000-fold more active analog S14G-HN showed no apparent differences in secondary structure.
In plain wordsIn lab tests, humanin and two analogs, one inactive and one far more active, had no clear difference in structure.
Peptide
Humanin
Outcome
No significant changeSecondary structure of HN, S7A-HN and S14G-HN
Study
n: not reported in source
Aqueous phosphate solution at pH 6.0
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
Comparison of a parent HN peptide, an inactive analog (S7A-HN) and a 1000-fold more active analog (S14G-HN) showed no apparent differences in the secondary structure.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-1920003501
02LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism
In aqueous phosphate solution, HN, S7A-HN and S14G-HN were all disordered over a wide range of peptide concentration.
In plain wordsIn lab tests, humanin and two analogs had no fixed shape across a wide range of concentrations.
Peptide
Humanin
Outcome
Not applicableDisorder of HN, S7A-HN and S14G-HN
Study
n: not reported in source
Aqueous phosphate solution at pH 6.0
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
These peptides were all disordered over the wide range of peptide concentration.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-1920003502
03LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism
In 20 mM phosphate at pH 6.0, HN and S7A-HN aggregated into soluble oligomers.
In plain wordsIn lab tests, humanin and an inactive analog clumped into small soluble groups in 20 mM phosphate.
Peptide
Humanin
Outcome
Not applicableAggregation of HN and S7A-HN in 20 mM phosphate
Study
n: not reported in source
Aqueous phosphate solution at pH 6.0
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
Sedimentation analysis was done only for HN and S7A-HN and showed aggregation into soluble oligomers in 20 mM phosphate at pH 6.0.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-1920003503
04LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism
Aggregation of HN was greatly suppressed in 5 mM phosphate at pH 6.0 compared with 20 mM, with smaller oligomers.
In plain wordsIn lab tests, humanin clumped much less in 5 mM phosphate than in 20 mM phosphate.
Peptide
Humanin
Outcome
DecreaseAggregation of HN in 5 mM phosphate
Study
n: not reported in source
Aqueous phosphate solution at pH 6.0
Comparator
20 mM phosphate
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
Aggregation was greatly suppressed in 5 mM phosphate at the same pH in terms of aggregate size, with the formation of smaller oligomers.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-1920003504
05LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism
In 5 mM phosphate, the mass of the low molecular weight species of both HN and S7A-HN was close to monomers by sedimentation equilibrium.
In plain wordsIn lab tests, the small species of humanin and an inactive analog was close to the size of a single peptide.
Peptide
Humanin
Outcome
Not applicableMolecular mass of the low molecular weight species of HN and S7A-HN
Study
n: not reported in source
Aqueous phosphate solution at pH 6.0
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
The mass of this low molecular weight species was determined by sedimentation equilibrium to be close to monomers for both peptides.
In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.
Report a problemFND-1920003505

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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