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Study · Not a test of the peptide alone · 2008

6 results

Widely distributed residues in thymosin beta4 are critical for actin binding.

Au JK, Olivares AO, Henn A, Cao W, Safer D, De La Cruz EM

Biochemistry8 Mar 2008Cited by 6 (Europe PMC)

PMID18327913 (opens in a new tab)PMCIDPMC2587058 (opens in a new tab)DOI10.1021/bi701769u (opens in a new tab)PepBankPUB-18327913
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone6

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    Alanine substitution of Pro4 or Pro27 in thymosin beta4 reduced actin binding affinity by 3-fold or less.

    In plain wordsIn lab tests, changing two proline sites in thymosin beta4 had little effect on how tightly it binds actin.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeActin binding affinity of Pro4 and Pro27 mutants
    Study

    n: not reported in source

    MgATP-actin monomers
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    Reduction in actin binding affinity
    ≤ 3 fold
    Pro4 or Pro27 mutants

    Source sentences

    Summary
    Mutagenesis of Pro4 or Pro27 has little effect (<or=3-fold reduction) on the actin binding affinity of Tbeta4.
    1 context sentence
    Summary · context
    We have investigated the contributions of hydrophobic residues, the conserved and variable proline residues, and the conserved lysine residues to the affinity and kinetics of thymosin beta4 (Tbeta4) binding to MgATP-actin monomers.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  2. 02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    Alanine substitution of Lys18 and Lys19, Leu28, Pro29 or Ile34 weakened the affinity of the actin-thymosin beta4 complex by 10-fold or more.

    In plain wordsIn lab tests, changing several sites in thymosin beta4 made its grip on actin much weaker.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseActin binding affinity of Lys18/Lys19, Leu28, Pro29 and Ile34 mutants
    Study

    n: not reported in source

    MgATP-actin monomers
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    Reduction in affinity of actin-Tbeta4 complex
    ≥ 10 fold
    Lys18/Lys19, Leu28, Pro29 or Ile34 mutants

    Source sentences

    Summary
    Substitution of Lys18 and Lys19, Leu28, Pro29, or Ile34 weakens the affinity of the actin-Tbeta4 complex >or=10-fold, but the kinetic basis of the lower stability varies among the mutants.
    1 context sentence
    Summary · context
    We have investigated the contributions of hydrophobic residues, the conserved and variable proline residues, and the conserved lysine residues to the affinity and kinetics of thymosin beta4 (Tbeta4) binding to MgATP-actin monomers.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  3. 03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    Substitution of the conserved lysine residues weakened thymosin beta4 binding to actin by slowing association and accelerating dissociation.

    In plain wordsIn lab tests, changing the lysine sites weakened actin binding because binding started slower and ended faster.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseKinetics of conserved lysine mutants
    Study

    n: not reported in source

    MgATP-actin monomers
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Substitution of the conserved lysine residues weakens the affinity by slowing association and accelerating dissociation.
    1 context sentence
    Summary · context
    We have investigated the contributions of hydrophobic residues, the conserved and variable proline residues, and the conserved lysine residues to the affinity and kinetics of thymosin beta4 (Tbeta4) binding to MgATP-actin monomers.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  4. 04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    Substitution of hydrophobic residue Leu28 or Ile34 weakened thymosin beta4 binding to actin by accelerating dissociation.

    In plain wordsIn lab tests, changing the Leu28 or Ile34 site weakened actin binding because the complex came apart faster.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseKinetics of Leu28 and Ile34 mutants
    Study

    n: not reported in source

    MgATP-actin monomers
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Substitution of hydrophobic residue Leu28 or Ile34 weakens the affinity by accelerating dissociation.
    1 context sentence
    Summary · context
    We have investigated the contributions of hydrophobic residues, the conserved and variable proline residues, and the conserved lysine residues to the affinity and kinetics of thymosin beta4 (Tbeta4) binding to MgATP-actin monomers.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  5. 05LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    Mutagenesis of Pro29 slowed the isomerization equilibrium of thymosin beta4 binding to actin.

    In plain wordsIn lab tests, changing the Pro29 site slowed a step in how thymosin beta4 locks onto actin.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseIsomerization equilibrium of Pro29 mutant
    Study

    n: not reported in source

    MgATP-actin monomers
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The isomerization equilibrium is slowed by mutagenesis of Pro29, as revealed by the double-exponential time course of association.
    1 context sentence
    Summary · context
    We have investigated the contributions of hydrophobic residues, the conserved and variable proline residues, and the conserved lysine residues to the affinity and kinetics of thymosin beta4 (Tbeta4) binding to MgATP-actin monomers.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  6. 06LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    Mutagenesis of Pro4 or Pro27 accelerated binding and dissociation of thymosin beta4 from actin but minimally affected binding affinity.

    In plain wordsIn lab tests, changing the Pro4 or Pro27 site sped up binding and release but barely changed how tight the grip was.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseBinding and dissociation rates of Pro4 and Pro27 mutants
    Study

    n: not reported in source

    MgATP-actin monomers
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Mutagenesis of Pro4 or Pro27 accelerates binding and dissociation but minimally affects the binding affinity (<or=3-fold reduction), suggesting that cis- trans isomerization of these proline residues contributes to the slow association rate constant of wild-type Tbeta4.
    1 context sentence
    Summary · context
    We have investigated the contributions of hydrophobic residues, the conserved and variable proline residues, and the conserved lysine residues to the affinity and kinetics of thymosin beta4 (Tbeta4) binding to MgATP-actin monomers.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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