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Study · In vitro (cells) · 2008

5 results

Molecular cloning and pharmacological characterization of porcine melanocortin-3 receptor.

Fan ZC, Sartin JL, Tao YX

The Journal of endocrinology1 Jan 2008Cited by 28 (Europe PMC)

PMID18180325 (opens in a new tab)DOI10.1677/joe-07-0403 (opens in a new tab)PepBankPUB-18180325
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In cells expressing the porcine MC3R, NDP-MSH bound with the highest affinity, ahead of D-Trp(8)-gamma-MSH, gamma-MSH and alpha-MSH.

    In plain wordsIn lab cells with the pig receptor, NDP-MSH bound the receptor more tightly than the other three ligands tested.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableBinding affinity of NDP-MSH at porcine MC3R
    Study

    n: not reported in source

    Cells expressing porcine MC3R
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The results showed that pMC3R bound NDP-MSH with the highest affinity followed by D-Trp(8)-gammaMSH, gamma-, and alpha-MSH.
    1 context sentence
    Summary · context
    With human MC3R (hMC3R) as a control, the binding and signaling properties of pMC3R were investigated using several agonists including alpha- and gamma-melanocyte-stimulating hormone (alpha- and gamma-MSH), D-Trp(8)-gamma-MSH, and [Nle(4)-D-Phe(7)]-MSH (NDP-MSH) and the natural antagonist agouti-related protein (AgRP).
  2. 02LabIn vitro (cells)Activity measurement

    In cells expressing the human MC3R, the binding affinity ranking of the ligands was the same as at the porcine receptor, with NDP-MSH highest.

    In plain wordsIn lab cells with the human receptor, NDP-MSH again bound the receptor most tightly of the ligands tested.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableBinding affinity ranking of NDP-MSH at human MC3R
    Study

    n: not reported in source

    Cells expressing human MC3R
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The same ranking was also found for hMC3R, although pMC3R had two- to ninefold higher affinities for these ligands.
    2 context sentences
    Summary · context
    The results showed that pMC3R bound NDP-MSH with the highest affinity followed by D-Trp(8)-gammaMSH, gamma-, and alpha-MSH.
    Summary · context
    With human MC3R (hMC3R) as a control, the binding and signaling properties of pMC3R were investigated using several agonists including alpha- and gamma-melanocyte-stimulating hormone (alpha- and gamma-MSH), D-Trp(8)-gamma-MSH, and [Nle(4)-D-Phe(7)]-MSH (NDP-MSH) and the natural antagonist agouti-related protein (AgRP).
  3. 03LabIn vitro (cells)Activity measurement

    In cells expressing the porcine MC3R, NDP-MSH ranked second in potency to stimulate cAMP generation, after D-Trp(8)-gamma-MSH and ahead of alpha-MSH and gamma-MSH.

    In plain wordsIn lab cells with the pig receptor, NDP-MSH was the second most potent ligand at raising a cell signal.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicablecAMP potency of NDP-MSH at porcine MC3R
    Study

    n: not reported in source

    Cells expressing porcine MC3R
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    D-Trp(8)-gamma-MSH was the most potent agonist to stimulate cAMP generation followed by NDP-, alpha-, and gamma-MSH.
    1 context sentence
    Summary · context
    With human MC3R (hMC3R) as a control, the binding and signaling properties of pMC3R were investigated using several agonists including alpha- and gamma-melanocyte-stimulating hormone (alpha- and gamma-MSH), D-Trp(8)-gamma-MSH, and [Nle(4)-D-Phe(7)]-MSH (NDP-MSH) and the natural antagonist agouti-related protein (AgRP).
  4. 04LabIn vitro (cells)Activity measurement

    In cells expressing the human MC3R, the cAMP potency ranking was the same as at the porcine receptor, with NDP-MSH second.

    In plain wordsIn lab cells with the human receptor, NDP-MSH was again the second most potent ligand at raising a cell signal.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicablecAMP potency ranking of NDP-MSH at human MC3R
    Study

    n: not reported in source

    Cells expressing human MC3R
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    D-Trp(8)-gamma-MSH was the most potent agonist to stimulate cAMP generation followed by NDP-, alpha-, and gamma-MSH. This ranking was the same as that of hMC3R.
    1 context sentence
    Summary · context
    With human MC3R (hMC3R) as a control, the binding and signaling properties of pMC3R were investigated using several agonists including alpha- and gamma-melanocyte-stimulating hormone (alpha- and gamma-MSH), D-Trp(8)-gamma-MSH, and [Nle(4)-D-Phe(7)]-MSH (NDP-MSH) and the natural antagonist agouti-related protein (AgRP).

Studies that did not test the peptide alone1

In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan I (afamelanotide).

  1. 05LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Activity measurement

    Across the tested melanocortin ligands pooled, the porcine MC3R had higher affinities than the human MC3R.

    In plain wordsIn lab cells, the pig receptor held the tested ligands more tightly than the human receptor did.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableLigand affinity at porcine versus human MC3R (pooled ligands)
    Study

    n: not reported in source

    Cells expressing porcine or human MC3R
    Comparator
    Human MC3R
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The same ranking was also found for hMC3R, although pMC3R had two- to ninefold higher affinities for these ligands.
    1 context sentence
    Summary · context
    With human MC3R (hMC3R) as a control, the binding and signaling properties of pMC3R were investigated using several agonists including alpha- and gamma-melanocyte-stimulating hormone (alpha- and gamma-MSH), D-Trp(8)-gamma-MSH, and [Nle(4)-D-Phe(7)]-MSH (NDP-MSH) and the natural antagonist agouti-related protein (AgRP).

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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