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Study · Measured the body's own peptide · 2007

5 results

Thymosin beta 4 expression and nuclear transport are regulated by hMLH1.

Brieger A, Plotz G, Zeuzem S, Trojan J

Biochemical and biophysical research communications15 Oct 2007Cited by 35 (Europe PMC)

PMID17967441 (opens in a new tab)DOI10.1016/j.bbrc.2007.10.010 (opens in a new tab)PepBankPUB-17967441
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Natural levels measured4

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 01LabEndogenous levelIn vitro (cells)Mechanism

    In hMLH1-deficient 293T and HCT-116 cells, reconstitution of hMLH1 increased expression of the body's own Tbeta4, which was weak before.

    In plain wordsIn human cell lines, restoring hMLH1 raised the body's own Tbeta4 levels, which were weak before.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseTbeta4 expression after hMLH1 reconstitution
    Study

    humann: not reported in source

    hMLH1-deficient and hMLH1-proficient human cell lines
    Comparator
    hMLH1-deficient cells
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    Tbeta4 was weakly expressed in the hMLH1-deficient cell lines 293T and HCT-116. Reconstitution of hMLH1 resulted in strong expression of Tbeta4.
    1 context sentence
    Summary · context
    We now identified Thymosin beta4 (Tbeta4), an actin-binding and cell motility regulating protein, by bacterial two-hybrid screening.

    This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.

  2. 02LabEndogenous levelIn vitro (cells)Mechanism

    In cells, confocal microscopy showed nuclear colocalization of the body's own Tbeta4 and hMLH1.

    In plain wordsIn cells, the body's own Tbeta4 and hMLH1 were found together in the cell nucleus.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableNuclear colocalization of Tbeta4 and hMLH1
    Study

    humann: not reported in source

    hMLH1-deficient and hMLH1-proficient human cell lines
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    Confocal laser microscopy revealed nuclear colocalization of both proteins.
    1 context sentence
    Summary · context
    We now identified Thymosin beta4 (Tbeta4), an actin-binding and cell motility regulating protein, by bacterial two-hybrid screening.

    This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.

  3. 03LabEndogenous levelIn vitro (cells)Mechanism

    In cells, hMLH1 mutants lacking a functional nuclear localization sequence caused cytoplasmic retention of both hMLH1 and the body's own Tbeta4.

    In plain wordsIn cells, hMLH1 mutants without a working nuclear signal kept both hMLH1 and the body's own Tbeta4 outside the nucleus.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableTbeta4 localization with hMLH1 nuclear localization sequence mutants
    Study

    humann: not reported in source

    hMLH1-deficient and hMLH1-proficient human cell lines
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    Reconstitution with hMLH1 mutants lacking a functional nuclear localization sequence resulted in cytoplasmatic retention of both proteins.
    1 context sentence
    Summary · context
    We now identified Thymosin beta4 (Tbeta4), an actin-binding and cell motility regulating protein, by bacterial two-hybrid screening.

    This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.

  4. 04LabEndogenous levelIn vitro (cells)Mechanism

    In cells, a bacterial two-hybrid screen identified Tbeta4 as an hMLH1 partner, and coimmunoprecipitation confirmed the interaction.

    In plain wordsIn lab tests, the body's own Tbeta4 was found to bind hMLH1, and a second test confirmed it.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableInteraction of Tbeta4 with hMLH1
    Study

    humann: not reported in source

    hMLH1-deficient and hMLH1-proficient human cell lines
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    We now identified Thymosin beta4 (Tbeta4), an actin-binding and cell motility regulating protein, by bacterial two-hybrid screening. Interaction was confirmed by coimmunoprecipitation.
    1 context sentence
    Summary · context
    We now identified Thymosin beta4 (Tbeta4), an actin-binding and cell motility regulating protein, by bacterial two-hybrid screening.

    This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.

Studies that did not test the peptide alone1

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 05LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In hMLH1-proficient cells, Tbeta4 or hMLH1 siRNA markedly decreased cell migration.

    In plain wordsIn cells, blocking the body's own Tbeta4 or hMLH1 with siRNA clearly reduced cell movement.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseCell migration after Tbeta4 or hMLH1 siRNA
    Study

    humann: not reported in source

    hMLH1-deficient and hMLH1-proficient human cell lines
    Outcome group
    Tumor growth · Cancer

    Source sentences

    Summary
    After Tbeta4- or hMLH1-siRNA treatment, cell migration of hMLH1-proficient cells was markedly decreased.
    1 context sentence
    Summary · context
    We now identified Thymosin beta4 (Tbeta4), an actin-binding and cell motility regulating protein, by bacterial two-hybrid screening.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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