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Study · In vitro (cells) · 2007

7 results

Expression of a functional g protein-coupled receptor 54-kisspeptin autoregulatory system in hypothalamic gonadotropin-releasing hormone neurons.

Quaynor S, Hu L, Leung PK, Feng H, Mores N, Krsmanovic LZ, Catt KJ

Molecular endocrinology (Baltimore, Md.)14 Aug 2007Cited by 57 (Europe PMC)

PMID17698953 (opens in a new tab)DOI10.1210/me.2007-0207 (opens in a new tab)PepBankPUB-17698953
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In identified GnRH neurons, kisspeptin caused an initial depolarization of the membrane potential.

    In plain wordsIn lab tests on brain cells, kisspeptin first made the cell membrane more electrically active.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseMembrane potential of GnRH neurons
    Study

    n: not reported in source

    Identified hypothalamic GnRH neurons (patch clamp)
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Whole cell patch-clamp recordings from identified GnRH neurons showed initial depolarizing effects of kisspeptin on membrane potential
  2. 02LabIn vitro (cells)Activity measurement

    In identified GnRH neurons, kisspeptin depolarization was followed by increased action potential firing.

    In plain wordsIn lab tests on brain cells, kisspeptin was followed by more electrical firing.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseAction potential firing of GnRH neurons
    Study

    n: not reported in source

    Identified hypothalamic GnRH neurons (patch clamp)
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Whole cell patch-clamp recordings from identified GnRH neurons showed initial depolarizing effects of kisspeptin on membrane potential, followed by increased action potential firing.
  3. 03LabIn vitro (cells)Activity measurement

    In perifused GT1-7 neuronal cells, kisspeptin‑10 increased GnRH peak amplitude.

    In plain wordsIn lab cells, kisspeptin‑10 made the GnRH release peaks higher.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseGnRH peak amplitude in GT1-7 cells
    Study

    n: not reported in source

    GT1-7 neuronal cells (perifusion)
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In perifusion studies, treatment of GT1-7 neuronal cells with kisspeptin-10 increased GnRH peak amplitude and duration.
  4. 04LabIn vitro (cells)Activity measurement

    In perifused GT1-7 neuronal cells, kisspeptin‑10 increased GnRH peak duration.

    In plain wordsIn lab cells, kisspeptin‑10 made the GnRH release peaks last longer.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseGnRH peak duration in GT1-7 cells
    Study

    n: not reported in source

    GT1-7 neuronal cells (perifusion)
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In perifusion studies, treatment of GT1-7 neuronal cells with kisspeptin-10 increased GnRH peak amplitude and duration.

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 05LabEndogenous levelIn vitro (cells)Mechanism

    RNA extracts from individually identified hypothalamic GnRH neurons expressed GnRH, kisspeptin-1 and GPR54.

    In plain wordsIn lab tests, brain cells that make GnRH also made the body's own kisspeptin and its receptor.

    Peptide
    Kisspeptin-10
    Outcome
    Not applicableKisspeptin-1 and GPR54 mRNA expression in GnRH neurons
    Study

    n: not reported in source

    Individually identified hypothalamic GnRH neurons
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    Analysis of RNA extracts from individually identified hypothalamic GnRH neurons with primers for GnRH, kisspeptin-1, and GPR54 revealed expression of all three gene products.

    This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.

  2. 06LabEndogenous levelIn vitro (cells)Mechanism

    A specific RIA detected the production and secretion of kisspeptin in cultured hypothalamic neurons and GT1-7 cells.

    In plain wordsIn lab cells, tests found that the cells make and release the body's own kisspeptin.

    Peptide
    Kisspeptin-10
    Outcome
    Not applicableKisspeptin production and secretion
    Study

    n: not reported in source

    Cultured hypothalamic neurons and GT1-7 cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    The production and secretion of kisspeptin in cultured hypothalamic neurons and GT1-7 cells were detected by a specific RIA

    This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.

  3. 07LabEndogenous levelIn vitro (cells)Mechanism

    GnRH treatment significantly reduced kisspeptin secretion in cultured hypothalamic neurons and GT1-7 cells.

    In plain wordsIn lab cells, GnRH lowered how much of the body's own kisspeptin the cells released.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseKisspeptin secretion after GnRH treatment
    Study

    n: not reported in source

    Cultured hypothalamic neurons and GT1-7 cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    The production and secretion of kisspeptin in cultured hypothalamic neurons and GT1-7 cells were detected by a specific RIA and was significantly reduced by treatment with GnRH.

    This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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