Direct pituitary effects of kisspeptin: activation of gonadotrophs and somatotrophs and stimulation of luteinising hormone and growth hormone secretion.

Gutiérrez-Pascual E, Martínez-Fuentes AJ, Pinilla L, Tena-Sempere M, Malagón MM, Castaño JP

Journal of neuroendocrinology1 Jul 2007Cited by 138 (Europe PMC)

PMID17532794DOI10.1111/j.1365-2826.2007.01558.xPepBankPUB-17532794

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

10 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In male rat pituitary cells, kisspeptin‑10 raised free cytosolic Ca2+ in approximately 10% of cells.
In plain wordsIn rat pituitary cells in the lab, kisspeptin‑10 raised calcium inside about one in ten cells.
Peptide
Kisspeptin-10
Outcome
IncreaseCytosolic Ca2+ rise in male pituitary cells
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Measurements
Responding cells
≈ 10%
male rat pituitary cells
Source sentences
Summary
Moreover, kisspeptin-10 induced a rise in free cytosolic Ca(2+) concentration ([Ca(2+)](i)) in approximately 10% of male rat pituitary cells.
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
Report a problemFND-1753279402
02LabIn vitro (cells)Activity measurement
In rat pituitary gonadotrophs, kisspeptin‑10 raised cytosolic Ca2+ by 62.8%.
In plain wordsIn rat pituitary cells in the lab, kisspeptin‑10 raised calcium inside LH-producing cells.
Peptide
Kisspeptin-10
Outcome
IncreaseCytosolic Ca2+ rise in gonadotrophs
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Measurements
Cytosolic Ca2+ rise
62.8%
gonadotrophs
Source sentences
Summary
gonadotrophs, in which a 62.8 +/- 16.0%[Ca(2+)](i) rise was observed
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
Report a problemFND-1753279403
03LabIn vitro (cells)Activity measurement
In rat pituitary somatotrophs, kisspeptin‑10 raised cytosolic Ca2+ by 60.3%.
In plain wordsIn rat pituitary cells in the lab, kisspeptin‑10 raised calcium inside growth hormone-producing cells.
Peptide
Kisspeptin-10
Outcome
IncreaseCytosolic Ca2+ rise in somatotrophs
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Measurements
Cytosolic Ca2+ rise
60.3%
somatotrophs
Source sentences
Summary
but also somatotrophs, wherein kisspeptin induced a 60.3 +/- 5.5%[Ca(2+)](i) increase.
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
Report a problemFND-1753279404
04LabIn vitro (cells)Activity measurement
In dispersed rat pituitary cells, increasing kisspeptin‑10 concentrations induced dose-related LH secretion, of lower magnitude than GnRH.
In plain wordsIn rat pituitary cells in the lab, more kisspeptin‑10 gave more LH release, less than GnRH gave.
Peptide
Kisspeptin-10
Outcome
IncreaseLH secretion, dose-response
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
challenge of dispersed pituitary cells with increasing kisspeptin-10 concentrations induced dose-related LH and growth hormone (GH) secretory responses
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
Report a problemFND-1753279405
05LabIn vitro (cells)Activity measurement
In dispersed rat pituitary cells, increasing kisspeptin‑10 concentrations induced dose-related GH secretion, of lower magnitude than GH-releasing hormone.
In plain wordsIn rat pituitary cells in the lab, more kisspeptin‑10 gave more growth hormone release, less than GHRH gave.
Peptide
Kisspeptin-10
Outcome
IncreaseGH secretion, dose-response
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
challenge of dispersed pituitary cells with increasing kisspeptin-10 concentrations induced dose-related LH and growth hormone (GH) secretory responses
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
Report a problemFND-1753279406
06LabIn vitro (cells)Activity measurement
In male rat pituitary cells, 10(-8) M kisspeptin caused a maximal LH release of 218.7%.
In plain wordsIn male rat pituitary cells in the lab, kisspeptin strongly raised LH release above the starting level.
Peptide
Kisspeptin-10
Outcome
IncreaseMaximal LH release, male cells
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10(-8) MAs given in this study (rat).
Measurements
LH release
218.7%
male rat pituitary cells
Source sentences
Summary
In particular, 10(-8) M kisspeptin caused maximal increases in LH release (218.7 +/- 23.6% and 180.4 +/- 7.2% in male and female rat pituitary cells, respectively), and also stimulated maximally GH secretion (181.9 +/- 14.9% and 260.2 +/- 15.9% in male and female rat pituitary cells, respectively).
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
Report a problemFND-1753279407
07LabIn vitro (cells)Activity measurement
In female rat pituitary cells, 10(-8) M kisspeptin caused a maximal LH release of 180.4%.
In plain wordsIn female rat pituitary cells in the lab, kisspeptin raised LH release well above the starting level.
Peptide
Kisspeptin-10
Outcome
IncreaseMaximal LH release, female cells
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
10(-8) MAs given in this study (rat).
Measurements
LH release
180.4%
female rat pituitary cells
Source sentences
Summary
In particular, 10(-8) M kisspeptin caused maximal increases in LH release (218.7 +/- 23.6% and 180.4 +/- 7.2% in male and female rat pituitary cells, respectively), and also stimulated maximally GH secretion (181.9 +/- 14.9% and 260.2 +/- 15.9% in male and female rat pituitary cells, respectively).
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
Report a problemFND-1753279408
08LabIn vitro (cells)Activity measurement
In male rat pituitary cells, 10(-8) M kisspeptin maximally stimulated GH secretion to 181.9%.
In plain wordsIn male rat pituitary cells in the lab, kisspeptin raised growth hormone release well above the starting level.
Peptide
Kisspeptin-10
Outcome
IncreaseMaximal GH release, male cells
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
10(-8) MAs given in this study (rat).
Measurements
GH release
181.9%
male rat pituitary cells
Source sentences
Summary
In particular, 10(-8) M kisspeptin caused maximal increases in LH release (218.7 +/- 23.6% and 180.4 +/- 7.2% in male and female rat pituitary cells, respectively), and also stimulated maximally GH secretion (181.9 +/- 14.9% and 260.2 +/- 15.9% in male and female rat pituitary cells, respectively).
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
Report a problemFND-1753279409
09LabIn vitro (cells)Activity measurement
In female rat pituitary cells, 10(-8) M kisspeptin maximally stimulated GH secretion to 260.2%.
In plain wordsIn female rat pituitary cells in the lab, kisspeptin raised growth hormone release far above the starting level.
Peptide
Kisspeptin-10
Outcome
IncreaseMaximal GH release, female cells
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
10(-8) MAs given in this study (rat).
Measurements
GH release
260.2%
female rat pituitary cells
Source sentences
Summary
In particular, 10(-8) M kisspeptin caused maximal increases in LH release (218.7 +/- 23.6% and 180.4 +/- 7.2% in male and female rat pituitary cells, respectively), and also stimulated maximally GH secretion (181.9 +/- 14.9% and 260.2 +/- 15.9% in male and female rat pituitary cells, respectively).
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
Report a problemFND-1753279410
10LabIn vitro (cells)Activity measurement
In male rat pituitary cells, kisspeptin- and GnRH-induced LH responses showed moderate summation after short-term incubation.
In plain wordsIn male rat pituitary cells in the lab, kisspeptin and GnRH together gave a somewhat larger LH response than either alone.
Peptide
Kisspeptin-10
Outcome
IncreaseLH response with kisspeptin plus GnRH
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Additionally, moderate summation of kisspeptin- and GnRH-induced LH responses was observed after short-term incubation of male rat pituitary cells.
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
Report a problemFND-1753279411

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

11LabEndogenous levelIn vitro (cells)Mechanism
In peripubertal male and female rats, both KiSS-1 and its receptor GPR54 were expressed in the pituitary.
In plain wordsIn rats, the pituitary makes the body's own kisspeptin gene product and its receptor.
Peptide
Kisspeptin-10
Outcome
Not applicableKiSS-1 and GPR54 expression in pituitary
Study
ratn: not reported in source
Dispersed primary pituitary cell cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
The results obtained show that both GPR54 and KiSS-1 are expressed in the pituitary of peripubertal male and female rats.
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify this issue, cultures of rat pituitary cells were used to evaluate expression of KiSS-1 and GPR54, and to monitor the ability of kisspeptin-10 to stimulate Ca(2+) responses in gonadotrophs and to elicit luteinising hormone (LH) secretion in vitro.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-1753279401

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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MeSH terms

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