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Study · In vitro (cells) · 2007

11 results

Melanocortin-3 receptor activates MAP kinase via PI3 kinase.

Chai B, Li JY, Zhang W, Ammori JB, Mulholland MW

Regulatory peptides22 Dec 2006Cited by 42 (Europe PMC)

PMID17188372 (opens in a new tab)DOI10.1016/j.regpep.2006.11.003 (opens in a new tab)PepBankPUB-17188372
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

11 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, NDP-MSH phosphorylated ERK1/2 in a dose-dependent manner.

    In plain wordsIn lab cells with the human MC3R receptor, NDP-MSH switched on the ERK1/2 signal in a dose-dependent way.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseERK1/2 phosphorylation
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    EC50
    3.3 x 10(-)(9) M
    ERK1/2 phosphorylation

    Source sentences

    Summary
    ERK1/2 was phosphorylated in a dose-dependent manner with an EC(50) of 3.3+/-1.5 x 10(-)(9) M, similar to the IC(50) of NDP-MSH binding to the MC3R.
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).
  2. 02LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, the antagonist SHU9119 blocked the ERK1/2 phosphorylation induced by NDP-MSH.

    In plain wordsIn lab cells, the receptor blocker SHU9119 stopped the ERK1/2 signal that NDP-MSH produced.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseERK1/2 phosphorylation with SHU9119
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Comparator
    NDP-MSH without SHU9119
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    ERK1/2 phosphorylation was blocked by the melanocortin receptor antagonists SHU9119.
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).
  3. 03LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, NDP-MSH-induced ERK1/2 phosphorylation was sensitive to pertussis toxin.

    In plain wordsIn lab cells, pertussis toxin reduced the ERK1/2 signal that NDP-MSH produced.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseNDP-MSH-induced ERK1/2 phosphorylation with pertussis toxin
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Comparator
    NDP-MSH without pertussis toxin
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NDP-MSH-induced ERK1/2 phosphorylation was sensitive to pertussis toxin
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).
  4. 04LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, NDP-MSH-induced ERK1/2 phosphorylation was sensitive to the PI3K inhibitor wortmannin.

    In plain wordsIn lab cells, the PI3K blocker wortmannin reduced the ERK1/2 signal that NDP-MSH produced.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseNDP-MSH-induced ERK1/2 phosphorylation with wortmannin
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Comparator
    NDP-MSH without wortmannin
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NDP-MSH-induced ERK1/2 phosphorylation was sensitive to pertussis toxin and the PI3K inhibitor, wortmannin.
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).
  5. 05LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, Rp-cAMPS did not inhibit NDP-MSH-induced ERK1/2 phosphorylation.

    In plain wordsIn lab cells, Rp-cAMPS did not block the ERK1/2 signal that NDP-MSH produced.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeNDP-MSH-induced ERK1/2 phosphorylation with Rp-cAMPS
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Comparator
    NDP-MSH without Rp-cAMPS
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Rp-cAMPS, BAPTA-AM and Myr-PKC did not inhibit the NDP-MSH-induced ERK1/2 phosphorylation.
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).
  6. 06LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, BAPTA-AM did not inhibit NDP-MSH-induced ERK1/2 phosphorylation.

    In plain wordsIn lab cells, BAPTA-AM did not block the ERK1/2 signal that NDP-MSH produced.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeNDP-MSH-induced ERK1/2 phosphorylation with BAPTA-AM
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Comparator
    NDP-MSH without BAPTA-AM
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Rp-cAMPS, BAPTA-AM and Myr-PKC did not inhibit the NDP-MSH-induced ERK1/2 phosphorylation.
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).
  7. 07LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, Myr-PKC did not inhibit NDP-MSH-induced ERK1/2 phosphorylation.

    In plain wordsIn lab cells, Myr-PKC did not block the ERK1/2 signal that NDP-MSH produced.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeNDP-MSH-induced ERK1/2 phosphorylation with Myr-PKC
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Comparator
    NDP-MSH without Myr-PKC
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Rp-cAMPS, BAPTA-AM and Myr-PKC did not inhibit the NDP-MSH-induced ERK1/2 phosphorylation.
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).
  8. 08LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, NDP-MSH stimulated cellular proliferation in a dose-dependent manner.

    In plain wordsIn lab cells with the human MC3R receptor, NDP-MSH made cells multiply in a dose-dependent way.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseCellular proliferation
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    EC50
    2.1 x 10(-)(9) M
    cellular proliferation

    Source sentences

    Summary
    NDP-MSH stimulated cellular proliferation in a dose-dependent manner with a similar EC(50) to ERK1/2 phosphorylation, 2.1+/-0.6 x 10(-)(9) M.
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).
  9. 09LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, AGRP (86-132) blocked NDP-MSH-induced cellular proliferation.

    In plain wordsIn lab cells, AGRP (86-132) stopped the cell growth that NDP-MSH produced.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseNDP-MSH-induced proliferation with AGRP (86-132)
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Comparator
    NDP-MSH without AGRP
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Cellular proliferation was blocked by AGRP (86-132)
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).
  10. 10LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, the MEK inhibitor PD98059 blocked NDP-MSH-induced cellular proliferation.

    In plain wordsIn lab cells, the MEK blocker PD98059 stopped the cell growth that NDP-MSH produced.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseNDP-MSH-induced proliferation with MEK inhibitor PD98059
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Comparator
    NDP-MSH without PD98059
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Cellular proliferation was blocked by AGRP (86-132) and by the MEK inhibitor, PD98059.
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).
  11. 11LabIn vitro (cells)Mechanism

    In HEK 293 cells expressing human MC3R, NDP-MSH did not inhibit serum deprivation-induced apoptosis.

    In plain wordsIn lab cells, NDP-MSH did not prevent cell death caused by lack of serum.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeSerum deprivation-induced apoptosis
    Study

    humann: not reported in source

    HEK 293 cells stably expressing human MC3R
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The NDP-MSH did not inhibit serum deprivation-induced apoptosis.
    1 context sentence
    Summary · context
    HEK 293 cells stably expressing human melanocortin-3 receptor (MC3R) were exposed to melanocortin receptor agonist, NDP-MSH (10(-)(10)-10(-)(6) M).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

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