Regulation of GnRH I receptor gene expression by the GnRH agonist triptorelin, estradiol, and progesterone in the gonadotroph-derived cell line alphaT3-1.

Weiss JM, Polack S, Treeck O, Diedrich K, Ortmann O

Endocrine1 Aug 2006Cited by 12 (Europe PMC)

PMID17185802DOI10.1385/endo:30:1:139PepBankPUB-17185802

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

3 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In alphaT3-1 cells, continuous triptorelin reduced GnRH I receptor mRNA in a time- and concentration-dependent manner.
In plain wordsIn cells, continuous triptorelin reduced GnRH receptor mRNA as exposure time and concentration increased.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
DecreaseGnRH I receptor mRNA with continuous exposure
Study
n: not reported in source
alphaT3-1 gonadotroph-derived cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10 nM or 100 pM · 30 min, 3 h or 9 hAs given in this study.
Source sentences
Summary
GnRH I-R mRNA from cells treated with continuous triptorelin decreased in a time- and concentration-dependent manner.
2 context sentences(design, population, supporting numbers)
Summary · context
To elucidate whether this regulation is due to alterations at the transcriptional level, we examined the GnRH I-R mRNA expression in the gonadotroph-derived cell line alphaT3-1 treated with different estradiol and progesterone paradigms and the GnRH I agonist triptorelin.
Summary · context
Cells were exposed to 10 nM or 100 pM triptorelin for 30 min, 3 h, 9 h, or, in pulsatile way, with a 5-min pulse per hour.
Report a problemFND-1718580201
02LabIn vitro (cells)Mechanism
In alphaT3-1 cells, pulsatile triptorelin increased GnRH I receptor gene expression.
In plain wordsIn cells, pulsatile triptorelin increased GnRH receptor gene expression.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
IncreaseGnRH I receptor expression with pulsatile exposure
Study
n: not reported in source
alphaT3-1 gonadotroph-derived cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10 nM or 100 pM · 5-min pulse per hourAs given in this study.
Source sentences
Summary
Pulsatile triptorelin increased GnRH I-R gene expression.
2 context sentences(design, population, supporting numbers)
Summary · context
To elucidate whether this regulation is due to alterations at the transcriptional level, we examined the GnRH I-R mRNA expression in the gonadotroph-derived cell line alphaT3-1 treated with different estradiol and progesterone paradigms and the GnRH I agonist triptorelin.
Summary · context
Cells were exposed to 10 nM or 100 pM triptorelin for 30 min, 3 h, 9 h, or, in pulsatile way, with a 5-min pulse per hour.
Report a problemFND-1718580202
03LabIn vitro (cells)Mechanism
In alphaT3-1 cells, added triptorelin did not alter steroid-induced GnRH I receptor mRNA suppression.
In plain wordsIn cells, added triptorelin did not alter receptor mRNA suppression compared with steroid treatment alone.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
No significant changeEffect on steroid-induced receptor mRNA suppression
Study
n: not reported in source
alphaT3-1 gonadotroph-derived cells
Comparator
Steroid treatment alone
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10 nM · 30 min or 3 hAs given in this study.
Source sentences
Summary
The addition of 10 nM triptorelin for 30 min or 3 h could not influence that steroid effect.
3 context sentences(design, population, supporting numbers)
Summary · context
Continuous combined treatment with estradiol and progesterone lead to a significant decrease of GnRH I-R mRNA by 30% and by 35% for estradiol alone.
Summary · context
To elucidate whether this regulation is due to alterations at the transcriptional level, we examined the GnRH I-R mRNA expression in the gonadotroph-derived cell line alphaT3-1 treated with different estradiol and progesterone paradigms and the GnRH I agonist triptorelin.
Summary · context
Cells were exposed to 10 nM or 100 pM triptorelin for 30 min, 3 h, 9 h, or, in pulsatile way, with a 5-min pulse per hour.
Report a problemFND-1718580206

Studies that did not test the peptide alone3

In this study, Triptorelin (Trelstar, Decapeptyl) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Triptorelin (Trelstar, Decapeptyl).

04LabNot a test of Triptorelin (Trelstar, Decapeptyl) aloneIn vitro (cells)Mechanism
In alphaT3-1 cells, progesterone enhanced the receptor-expression increase associated with pulsatile triptorelin.
In plain wordsIn cells, progesterone enhanced the receptor-expression increase compared with pulsatile triptorelin alone.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
IncreaseProgesterone modulation of pulsatile response
Study
n: not reported in source
alphaT3-1 gonadotroph-derived cells
Comparator
Pulsatile triptorelin without progesterone
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Progesterone alone further enhanced this effect, whereas estradiol and its combination with progesterone diminished it.
3 context sentences(design, population, supporting numbers)
Summary · context
Pulsatile triptorelin increased GnRH I-R gene expression.
Summary · context
To elucidate whether this regulation is due to alterations at the transcriptional level, we examined the GnRH I-R mRNA expression in the gonadotroph-derived cell line alphaT3-1 treated with different estradiol and progesterone paradigms and the GnRH I agonist triptorelin.
Summary · context
Cells were exposed to 10 nM or 100 pM triptorelin for 30 min, 3 h, 9 h, or, in pulsatile way, with a 5-min pulse per hour.
In this study, Triptorelin (Trelstar, Decapeptyl) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Triptorelin (Trelstar, Decapeptyl).
Report a problemFND-1718580203
05LabNot a test of Triptorelin (Trelstar, Decapeptyl) aloneIn vitro (cells)Mechanism
In alphaT3-1 cells, estradiol diminished the receptor-expression increase associated with pulsatile triptorelin.
In plain wordsIn cells, estradiol reduced the receptor-expression increase compared with pulsatile triptorelin alone.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
DecreaseEstradiol modulation of pulsatile response
Study
n: not reported in source
alphaT3-1 gonadotroph-derived cells
Comparator
Pulsatile triptorelin without estradiol
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Progesterone alone further enhanced this effect, whereas estradiol and its combination with progesterone diminished it.
3 context sentences(design, population, supporting numbers)
Summary · context
Pulsatile triptorelin increased GnRH I-R gene expression.
Summary · context
To elucidate whether this regulation is due to alterations at the transcriptional level, we examined the GnRH I-R mRNA expression in the gonadotroph-derived cell line alphaT3-1 treated with different estradiol and progesterone paradigms and the GnRH I agonist triptorelin.
Summary · context
Cells were exposed to 10 nM or 100 pM triptorelin for 30 min, 3 h, 9 h, or, in pulsatile way, with a 5-min pulse per hour.
In this study, Triptorelin (Trelstar, Decapeptyl) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Triptorelin (Trelstar, Decapeptyl).
Report a problemFND-1718580204
06LabNot a test of Triptorelin (Trelstar, Decapeptyl) aloneIn vitro (cells)Mechanism
In alphaT3-1 cells, estradiol plus progesterone diminished the receptor-expression increase associated with pulsatile triptorelin.
In plain wordsIn cells, estradiol plus progesterone reduced the receptor-expression increase compared with pulsatile triptorelin alone.
Peptide
Triptorelin (Trelstar, Decapeptyl)
Outcome
DecreaseCombined steroid modulation of pulsatile response
Study
n: not reported in source
alphaT3-1 gonadotroph-derived cells
Comparator
Pulsatile triptorelin without combined steroids
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Progesterone alone further enhanced this effect, whereas estradiol and its combination with progesterone diminished it.
3 context sentences(design, population, supporting numbers)
Summary · context
Pulsatile triptorelin increased GnRH I-R gene expression.
Summary · context
To elucidate whether this regulation is due to alterations at the transcriptional level, we examined the GnRH I-R mRNA expression in the gonadotroph-derived cell line alphaT3-1 treated with different estradiol and progesterone paradigms and the GnRH I agonist triptorelin.
Summary · context
Cells were exposed to 10 nM or 100 pM triptorelin for 30 min, 3 h, 9 h, or, in pulsatile way, with a 5-min pulse per hour.
In this study, Triptorelin (Trelstar, Decapeptyl) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Triptorelin (Trelstar, Decapeptyl).
Report a problemFND-1718580205

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
22 Oct 2026, 02:26 UTC
Study results updated
Version 1
21 Oct 2026, 18:59 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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