In rat pituitary RC-4B/C cells, hexarelin caused a dose-dependent increase in intracellular calcium with an EC50 of 1.7 nM.
In plain wordsIn rat pituitary cells in the lab, hexarelin raised calcium inside the cells in a dose-dependent way.
- Peptide
- Hexarelin
- Outcome
- IncreaseIntracellular Ca2+ mobilization (EC50)
- Study
ratn: not reported in source
RC-4B/C rat pituitary adenoma cell line- Outcome group
- Receptor activity and mechanism · Endocrine and hormones
- Studied dose
- Dose not stated · added to cellsAs given in this study (rat).
Measurements
- EC50
- 1.7 nM
- hexarelin, Ca(2+) mobilization in RC-4B/C cells
Source sentences
In a Ca(2+)mobilization assay, RC-4B/C cells demonstrate a dose-dependent increase in intracellular [Ca(2+)] on stimulation with rat ghrelin and a related peptide agonist, hexarelin (EC(50), 1.0 nM and 1.7 nM respectively), but are unresponsive to treatment with inactive des-octanoyl rat ghrelin.
1 context sentence(design, population, supporting numbers)
We show here that a cell line derived from a rat anterior pituitary adenoma, RC-4B/C, expresses endogenous GHS-R as judged by reverse transcriptase-PCR.