Melanocortin-4 receptor-mediated inhibition of apoptosis in immortalized hypothalamic neurons via mitogen-activated protein kinase.

Chai B, Li JY, Zhang W, Newman E, Ammori J, Mulholland MW

Peptides27 Jun 2006Cited by 70 (Europe PMC)

PMID16806584DOI10.1016/j.peptides.2006.05.005PepBankPUB-16806584

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In GT1-1 cells, NDP-MSH showed specific binding and stimulated cAMP production, implying functional melanocortin receptor expression.
In plain wordsIn cells, NDP-MSH bound specifically and raised cAMP, which suggests working melanocortin receptors.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreaseNDP-MSH binding and cAMP production
Study
n: not reported in source
GT1-1 hypothalamic cell line
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
Functional melanocortin receptor expression was implied by specific binding of NDP-MSH and cAMP production.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, GT1-1 cells were used to study MC4R signaling pathways and to examine the effects of melanocortin receptor agonist NDP-MSH on apoptosis.
Report a problemFND-1680658401
02LabIn vitro (cells)Activity measurement
In GT1-1 cells, NDP-MSH stimulated GnRH release in a dose-dependent manner.
In plain wordsIn cells, NDP-MSH increased GnRH release in a dose-dependent way.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreaseGnRH release
Study
n: not reported in source
GT1-1 hypothalamic cell line
Outcome group
Sex hormones and fertility · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
NDP-MSH-stimulated GnRH release in a dose-dependent manner.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, GT1-1 cells were used to study MC4R signaling pathways and to examine the effects of melanocortin receptor agonist NDP-MSH on apoptosis.
Report a problemFND-1680658402
03LabIn vitro (cells)Efficacy outcome
In GT1-1 cells with serum deprivation-induced apoptosis, NDP-MSH inhibited the apoptosis.
In plain wordsIn cells, NDP-MSH reduced cell death caused by serum deprivation.
Peptide
Melanotan I (afamelanotide)
Outcome
DecreaseSerum deprivation-induced apoptosis
Study
n: not reported in source
GT1-1 hypothalamic cell line
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
Serum deprivation-induced apoptosis in GT1-1 cells, and the NDP-MSH inhibited this effect.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, GT1-1 cells were used to study MC4R signaling pathways and to examine the effects of melanocortin receptor agonist NDP-MSH on apoptosis.
Report a problemFND-1680658403
04LabIn vitro (cells)Mechanism
In GT1-1 cells, NDP-MSH stimulated ERK1/2 phosphorylation in a dose-dependent manner.
In plain wordsIn cells, NDP-MSH increased ERK1/2 phosphorylation in a dose-dependent way.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreaseERK1/2 phosphorylation
Study
n: not reported in source
GT1-1 hypothalamic cell line
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
NDP-MSH-stimulated ERK1/2 phosphorylation in a dose-dependent manner.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, GT1-1 cells were used to study MC4R signaling pathways and to examine the effects of melanocortin receptor agonist NDP-MSH on apoptosis.
Report a problemFND-1680658404

Studies that did not test the peptide alone3

In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan I (afamelanotide).

05LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism
In GT1-1 cells, the antagonist SHU9119 blocked the antiapoptotic action of NDP-MSH.
In plain wordsIn cells, the blocker SHU9119 stopped the anti-cell-death action of NDP-MSH.
Peptide
Melanotan I (afamelanotide)
Outcome
DecreaseAntiapoptotic effect of NDP-MSH with SHU9119
Study
n: not reported in source
GT1-1 hypothalamic cell line
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
The melanocortin receptor antagonist SHU9119 blocked the antiapoptotic actions of NDP-MSH
1 context sentence(design, population, supporting numbers)
Summary · context
In this study, GT1-1 cells were used to study MC4R signaling pathways and to examine the effects of melanocortin receptor agonist NDP-MSH on apoptosis.
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).
Report a problemFND-1680658405
06LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism
In GT1-1 cells, the MAP kinase inhibitor PD98059 significantly attenuated the antiapoptotic effect of NDP-MSH.
In plain wordsIn cells, the MAP kinase blocker PD98059 reduced the anti-cell-death effect of NDP-MSH.
Peptide
Melanotan I (afamelanotide)
Outcome
DecreaseAntiapoptotic effect of NDP-MSH with PD98059
Study
n: not reported in source
GT1-1 hypothalamic cell line
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
and the MAP kinase inhibitor PD98059 significantly attenuated the antiapoptotic effect.
2 context sentences(design, population, supporting numbers)
Summary · context
Serum deprivation-induced apoptosis in GT1-1 cells, and the NDP-MSH inhibited this effect.
Summary · context
In this study, GT1-1 cells were used to study MC4R signaling pathways and to examine the effects of melanocortin receptor agonist NDP-MSH on apoptosis.
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).
Report a problemFND-1680658406
07LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism
In GT1-1 cells, SHU9119 abolished NDP-MSH-stimulated ERK1/2 phosphorylation.
In plain wordsIn cells, SHU9119 removed the ERK1/2 phosphorylation caused by NDP-MSH.
Peptide
Melanotan I (afamelanotide)
Outcome
DecreaseNDP-MSH-induced ERK1/2 phosphorylation with SHU9119
Study
n: not reported in source
GT1-1 hypothalamic cell line
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
ERK1/2 phosphorylation could be abolished by SHU9119.
2 context sentences(design, population, supporting numbers)
Summary · context
NDP-MSH-stimulated ERK1/2 phosphorylation in a dose-dependent manner.
Summary · context
In this study, GT1-1 cells were used to study MC4R signaling pathways and to examine the effects of melanocortin receptor agonist NDP-MSH on apoptosis.
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).
Report a problemFND-1680658407

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

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