The action of extracellular NAD+ on gluconeogenesis in the perfused rat liver.

Martins AG, Constantin J, Bracht F, Kelmer-Bracht AM, Bracht A

Molecular and cellular biochemistry21 Apr 2006Cited by 7 (Europe PMC)

PMID16652226DOI10.1007/s11010-005-9101-7PepBankPUB-16652226

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

5 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueMechanism
In perfused rat liver, NAD+ transiently inhibited oxygen uptake concentration-dependently during antegrade perfusion, followed by stimulation.
In plain wordsIn lab tests with rat liver, NAD+ first reduced oxygen uptake and then increased it during antegrade perfusion.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
MixedAntegrade oxygen uptake
Study
ratn: not reported in source
Hemoglobin-free perfused liver
Outcome group
Liver fat and liver function · Liver
Studied dose
25-100 microM · portal vein infusionAs given in this study (rat).
Source sentences
Summary
NAD+ infusion into the portal vein (antegrade perfusion) produced a concentration-dependent (25-100 microM) transient inhibition of oxygen uptake and gluconeogenesis.
2 context sentences(design, population, supporting numbers)
Summary · context
For both parameters inhibition was followed by stimulation.
Summary · context
Hemoglobin-free rat liver perfusion in antegrade and retrograde modes was used with enzymatic determination of glucose production and polarographic assay of oxygen uptake.
Report a problemFND-1665222601
02LabEx vivo tissueMechanism
In perfused rat liver, NAD+ transiently inhibited gluconeogenesis concentration-dependently during antegrade perfusion, followed by stimulation.
In plain wordsIn lab tests with rat liver, NAD+ first reduced gluconeogenesis and then increased it during antegrade perfusion.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
MixedAntegrade gluconeogenesis
Study
ratn: not reported in source
Hemoglobin-free perfused liver
Outcome group
Liver fat and liver function · Liver
Studied dose
25-100 microM · portal vein infusionAs given in this study (rat).
Source sentences
Summary
NAD+ infusion into the portal vein (antegrade perfusion) produced a concentration-dependent (25-100 microM) transient inhibition of oxygen uptake and gluconeogenesis.
2 context sentences(design, population, supporting numbers)
Summary · context
For both parameters inhibition was followed by stimulation.
Summary · context
Hemoglobin-free rat liver perfusion in antegrade and retrograde modes was used with enzymatic determination of glucose production and polarographic assay of oxygen uptake.
Report a problemFND-1665222602
03LabEx vivo tissueMechanism
In perfused rat liver, NAD+ produced only transient oxygen uptake stimulation during retrograde perfusion.
In plain wordsIn lab tests with rat liver, NAD+ temporarily increased oxygen uptake during retrograde perfusion.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseRetrograde oxygen uptake
Study
ratn: not reported in source
Hemoglobin-free perfused liver
Outcome group
Liver fat and liver function · Liver
Studied dose
Dose not stated · hepatic vein infusionAs given in this study (rat).
Source sentences
Summary
NAD+ infusion into the hepatic vein (retrograde perfusion) produced only transient stimulations.
2 context sentences(design, population, supporting numbers)
Summary · context
NAD+ infusion into the portal vein (antegrade perfusion) produced a concentration-dependent (25-100 microM) transient inhibition of oxygen uptake and gluconeogenesis. For both parameters inhibition was followed by stimulation.
Summary · context
Hemoglobin-free rat liver perfusion in antegrade and retrograde modes was used with enzymatic determination of glucose production and polarographic assay of oxygen uptake.
Report a problemFND-1665222603
04LabEx vivo tissueMechanism
In perfused rat liver, NAD+ produced only transient gluconeogenesis stimulation during retrograde perfusion.
In plain wordsIn lab tests with rat liver, NAD+ temporarily increased gluconeogenesis during retrograde perfusion.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseRetrograde gluconeogenesis
Study
ratn: not reported in source
Hemoglobin-free perfused liver
Outcome group
Liver fat and liver function · Liver
Studied dose
Dose not stated · hepatic vein infusionAs given in this study (rat).
Source sentences
Summary
NAD+ infusion into the hepatic vein (retrograde perfusion) produced only transient stimulations.
2 context sentences(design, population, supporting numbers)
Summary · context
NAD+ infusion into the portal vein (antegrade perfusion) produced a concentration-dependent (25-100 microM) transient inhibition of oxygen uptake and gluconeogenesis. For both parameters inhibition was followed by stimulation.
Summary · context
Hemoglobin-free rat liver perfusion in antegrade and retrograde modes was used with enzymatic determination of glucose production and polarographic assay of oxygen uptake.
Report a problemFND-1665222604
05LabEx vivo tissueMechanism
In perfused rat liver, calcium-free perfusion restricted NAD+ action to small transient stimulations.
In plain wordsIn lab tests with rat liver, NAD+ produced only small temporary stimulations during calcium-free perfusion.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseAction during calcium-free perfusion
Study
ratn: not reported in source
Hemoglobin-free perfused liver
Outcome group
Liver fat and liver function · Liver
Studied dose
Dose not stated
Source sentences
Summary
During Ca2+-free perfusion the action of NAD+ was restricted to small transient stimulations.
1 context sentence(design, population, supporting numbers)
Summary · context
Hemoglobin-free rat liver perfusion in antegrade and retrograde modes was used with enzymatic determination of glucose production and polarographic assay of oxygen uptake.
Report a problemFND-1665222605

Studies that did not test the peptide alone1

In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NAD+ (nicotinamide adenine dinucleotide).

06LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneEx vivo tissueMechanism
In perfused rat liver, eicosanoid synthesis inhibitors inhibited or changed NAD+ action.
In plain wordsIn lab tests with rat liver, eicosanoid synthesis inhibitors reduced or changed NAD+ action.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
MixedAction with eicosanoid synthesis inhibitors
Study
ratn: not reported in source
Hemoglobin-free perfused liver
Outcome group
Liver fat and liver function · Liver
Source sentences
Summary
Inhibitors of eicosanoid synthesis with different specificities (indo-methacin, nordihydroguaiaretic acid, bromophenacyl bromide) either inhibited or changed the action of NAD+.
1 context sentence(design, population, supporting numbers)
Summary · context
Hemoglobin-free rat liver perfusion in antegrade and retrograde modes was used with enzymatic determination of glucose production and polarographic assay of oxygen uptake.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-1665222606

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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