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Study · In vitro (cells) · rat · 2006

8 results

Anti-apoptotic factor humanin is expressed in the testis and prevents cell-death in leydig cells during the first wave of spermatogenesis.

Colón E, Strand ML, Carlsson-Skwirut C, Wahlgren A, Svechnikov KV, Cohen P, Söder O

Journal of cellular physiology1 Aug 2006Cited by 46 (Europe PMC)

PMID16619233 (opens in a new tab)DOI10.1002/jcp.20672 (opens in a new tab)PepBankPUB-16619233
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In cultured Leydig cells from 10-day-old rats, rat humanin stimulated incorporation of [(3)H]TdR into DNA, which indicates promotion of cell survival rather than faster cell multiplication.

    In plain wordsIn rat cells grown in the lab, humanin raised DNA labeling in a way that points to better cell survival, not more cell division.

    Peptide
    Humanin
    Outcome
    IncreaseLeydig cell DNA synthesis ([3H]TdR incorporation)
    Study

    ratn: not reported in source

    Cultured Leydig cells from 10-day-old rats
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNr stimulated the incorporation of [(3)H]TdR into DNA of Leydig cells from 10-days-old rats, in a manner that indicated promotion of cell survival rather than an increase in the rate of cell multiplication.
  2. 02LabIn vitro (cells)Efficacy outcome

    Rat humanin promoted the survival of Leydig cells in culture.

    In plain wordsIn rat cells grown in the lab, humanin helped Leydig cells survive.

    Peptide
    Humanin
    Outcome
    IncreaseLeydig cell survival in culture
    Study

    ratn: not reported in source

    Cultured Leydig cells
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    This peptide promoted the survival of Leydig cells in culture
  3. 03LabIn vitro (cells)Efficacy outcome

    Rat humanin alone enhanced steroidogenesis by cultured Leydig cells from 10- to 40-day-old rats.

    In plain wordsIn rat cells grown in the lab, humanin alone increased steroid production by Leydig cells.

    Peptide
    Humanin
    Outcome
    IncreaseSteroidogenesis alone
    Study

    ratn: not reported in source

    Cultured Leydig cells from 10- to 40-day-old rats
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    This peptide also enhanced steroidogenesis by cultured Leydig cells from 10- to 40-day-old rats both alone

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 04AnimalEndogenous levelAnimal study (in vivo)Mechanism

    In rat testis, rat humanin was expressed at high levels in Leydig cells at 10 and 40 days of age and in more cell types at 60 days.

    In plain wordsIn rats, the body's own humanin was found in testis cells, mostly Leydig cells in young rats and more cell types at 60 days.

    Peptide
    Humanin
    Outcome
    Not applicableRat humanin expression in the testis by age
    Study

    ratn: not reported in source

    Rat testis
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    The Leydig cells of 10- and 40- day-old rats expressed this peptide at high levels; and in the testis of 60-day-old rats the expression of HNr expanded to include Leydig, endothelial, peritubular and germ cells.
    1 context sentence
    Summary · context
    In the present report, we have demonstrated by immunohistochemical analysis and Western blotting the pattern of expression of rat humanin (HNr) in the testis of 10- to 60-day-old rats.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  2. 05LabEndogenous levelIn vitro (cells)Mechanism

    Leydig cells isolated from 10-, 40- and 60-day-old rats released rat humanin into the culture medium.

    In plain wordsIn rat cells grown in the lab, Leydig cells released their own humanin into the medium.

    Peptide
    Humanin
    Outcome
    Not applicableRat humanin release into Leydig cell culture medium
    Study

    ratn: not reported in source

    Cultured Leydig cells isolated from rats
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    As monitored by Western blotting, HNr was released into the medium of cultures of Leydig cells isolated from 10-, 40-, and 60-days-old rats.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  3. 06LabEndogenous levelIn vitro (cells)Mechanism

    In cultured rat Leydig cells, expression of rat humanin increased in response to GH and IGF-I.

    In plain wordsIn rat cells grown in the lab, GH and IGF-I raised the cells' own humanin expression.

    Peptide
    Humanin
    Outcome
    IncreaseRat humanin expression after GH and IGF-I
    Study

    ratn: not reported in source

    Cultured Leydig cells isolated from rats
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    The expression of HNr in cultured Leydig cells increased in response to GH and IGF-I.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

Studies that did not test the peptide alone2

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 07LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome

    Rat humanin together with IGF-I enhanced steroidogenesis in cultured Leydig cells synergistically.

    In plain wordsIn rat cells grown in the lab, humanin and IGF-I together raised steroid production more than expected from each alone.

    Peptide
    Humanin
    Outcome
    IncreaseSteroidogenesis with IGF-I
    Study

    ratn: not reported in source

    Cultured Leydig cells from 10- to 40-day-old rats
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    synergistically with IGF-I.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  2. 08LabNot a test of Humanin aloneIn vitro (cells)Efficacy outcome

    Rat humanin interacted with IGF-I to stimulate DNA synthesis in cultured Leydig cells.

    In plain wordsIn rat cells grown in the lab, humanin and IGF-I together stimulated DNA synthesis.

    Peptide
    Humanin
    Outcome
    IncreaseDNA synthesis with IGF-I
    Study

    ratn: not reported in source

    Cultured Leydig cells
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    and interacted with IGF-I to stimulate DNA synthesis and steroidogenesis.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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