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Study · In vitro (cells) · 2005

5 results

Mitochondria-targeted peptide prevents mitochondrial depolarization and apoptosis induced by tert-butyl hydroperoxide in neuronal cell lines.

Zhao K, Luo G, Giannelli S, Szeto HH

Biochemical pharmacology10 Oct 2005Cited by 210 (Europe PMC)

PMID16216225 (opens in a new tab)DOI10.1016/j.bcp.2005.08.022 (opens in a new tab)PepBankPUB-16216225
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In neuronal cell lines exposed to tBHP, concurrent SS‑31 at below 1 nM significantly decreased intracellular ROS.

    In plain wordsIn cells, very low doses of SS‑31 reduced harmful reactive oxygen molecules caused by an oxidant.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseIntracellular ROS after tBHP
    Study

    n: not reported in source

    N2A and SH-SY5Y neuronal cell lines
    Outcome group
    Mitochondrial function · Nervous system and cognition
    Studied dose
    <1 nMAs given in this study.

    Source sentences

    Summary
    Concurrent treatment with <1 nM SS-31 (D-Arg-Dmt-Lys-Phe-NH2; Dmt = 2',6'-dimethyltyrosine) significantly decreased intracellular ROS, increased mitochondrial potential, and prevented tBHP-induced apoptosis.
    1 context sentence
    Summary · context
    In the present work, we examined the ability of a novel cell-penetrating, mitochondria-targeted peptide antioxidant in protecting against oxidant-induced mitochondrial dysfunction and apoptosis in two neuronal cell lines.
  2. 02LabIn vitro (cells)Activity measurement

    In neuronal cell lines exposed to tBHP, concurrent SS‑31 at below 1 nM increased mitochondrial potential.

    In plain wordsIn cells, very low doses of SS‑31 raised mitochondrial membrane potential after oxidant damage.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseMitochondrial potential after tBHP
    Study

    n: not reported in source

    N2A and SH-SY5Y neuronal cell lines
    Outcome group
    Mitochondrial function · Nervous system and cognition
    Studied dose
    <1 nMAs given in this study.

    Source sentences

    Summary
    Concurrent treatment with <1 nM SS-31 (D-Arg-Dmt-Lys-Phe-NH2; Dmt = 2',6'-dimethyltyrosine) significantly decreased intracellular ROS, increased mitochondrial potential, and prevented tBHP-induced apoptosis.
    1 context sentence
    Summary · context
    In the present work, we examined the ability of a novel cell-penetrating, mitochondria-targeted peptide antioxidant in protecting against oxidant-induced mitochondrial dysfunction and apoptosis in two neuronal cell lines.
  3. 03LabIn vitro (cells)Activity measurement

    In neuronal cell lines exposed to tBHP, concurrent SS‑31 at below 1 nM prevented apoptosis.

    In plain wordsIn cells, very low doses of SS‑31 prevented programmed cell death caused by an oxidant.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreasetBHP-induced apoptosis
    Study

    n: not reported in source

    N2A and SH-SY5Y neuronal cell lines
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    <1 nMAs given in this study.

    Source sentences

    Summary
    Concurrent treatment with <1 nM SS-31 (D-Arg-Dmt-Lys-Phe-NH2; Dmt = 2',6'-dimethyltyrosine) significantly decreased intracellular ROS, increased mitochondrial potential, and prevented tBHP-induced apoptosis.
    1 context sentence
    Summary · context
    In the present work, we examined the ability of a novel cell-penetrating, mitochondria-targeted peptide antioxidant in protecting against oxidant-induced mitochondrial dysfunction and apoptosis in two neuronal cell lines.
  4. 04LabIn vitro (cells)Pharmacokinetics

    In neuronal cell lines, intracellular concentrations of [3H]SS‑31 were 6-fold higher than extracellular concentrations.

    In plain wordsIn cells, SS‑31 built up inside the cells to 6 times the level outside.

    Peptide
    Elamipretide (SS-31)
    Outcome
    Not applicableCellular uptake of [3H]SS-31
    Study

    n: not reported in source

    N2A and SH-SY5Y neuronal cell lines
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Intracellular to extracellular concentration ratio
    6 fold
    [3H]SS-31

    Source sentences

    Summary
    Intracellular concentrations of [3H]SS-31 were 6-fold higher than extracellular concentrations.
    1 context sentence
    Summary · context
    In the present work, we examined the ability of a novel cell-penetrating, mitochondria-targeted peptide antioxidant in protecting against oxidant-induced mitochondrial dysfunction and apoptosis in two neuronal cell lines.
  5. 05LabIn vitro (cells)Pharmacokinetics

    In studies with isolated mitochondria, [3H]SS‑31 was concentrated approximately 5000-fold in the mitochondrial pellet.

    In plain wordsIn lab tests with isolated mitochondria, SS‑31 collected in the mitochondria at about 5000 times the outside level.

    Peptide
    Elamipretide (SS-31)
    Outcome
    Not applicableMitochondrial accumulation of [3H]SS-31
    Study

    n: not reported in source

    Isolated mitochondria
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Concentration in mitochondrial pellet
    ≈ 5000 fold
    isolated mitochondria

    Source sentences

    Summary
    Studies using isolated mitochondria revealed that [3H]SS-31 was concentrated approximately 5000-fold in the mitochondrial pellet.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 08:19 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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