Identification of the positive and negative cis-elements involved in modulating the constitutive expression of mouse thymosin beta4 gene.

Hsiao HL, Su Y

Molecular and cellular biochemistry1 Apr 2005Cited by 4 (Europe PMC)

PMID16010974DOI10.1007/s11010-005-7638-0PepBankPUB-16010974

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Other
Primer extension and bioinformatics showed that the first exon of the mouse Tbeta4 gene spans 56 bp.
In plain wordsIn lab tests, the first exon of the mouse Tbeta4 gene was found to span 56 base pairs.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableFirst exon size and cap site of mouse Tbeta4 gene
Study
mousen: not reported in source
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
first exon length
56 bp
mTbeta4 gene
Source sentences
Summary
We found that the first exon of mTbeta4 gene spans 56 bp with its cap site situated in a putative initiator highly similar to the consensus mammalian sequence.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1601097401
02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Activity measurement
In transient transfection assays, reporter expression driven by the -117 to +56 region of the mouse Tbeta4 gene was about 8-fold higher than that driven by the SV40 promoter.
In plain wordsIn cells, the mouse Tbeta4 gene promoter region drove about 8 times more reporter activity than the SV40 promoter.
Peptide
Thymosin beta-4 (human)
Outcome
IncreasePromoter activity of the -117 to +56 region versus SV40 promoter
Study
mousen: not reported in source
NIH3T3 cells, transient transfection
Comparator
SV40 promoter
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
reporter expression, fold versus SV40 promoter
8 fold
-117 to +56 region
Source sentences
Summary
Interestingly, expression level of the reporter gene driven by the -117 to +56 region of mTbeta4 gene was approximately 8-fold higher than that directed by the SV40 promoter and significant promoter activity was found to be associated with the smaller (-56 to +56) fragment.
1 context sentence(design, population, supporting numbers)
Summary · context
We previously showed that the -278 to +410 region of mouse thymosin beta4 (mT,beta4) gene supports high levels of reporter gene expression in NIH3T3 cells.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1601097402
03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
A nuclear protein-bound silencer lay between -167 and -118 and an enhancer lay in the -117 to -88 region of the mouse Tbeta4 gene, and neither affected SV40-driven expression.
In plain wordsIn cells, control elements of the mouse Tbeta4 gene promoter were found, but they did not change expression driven by the SV40 promoter.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableSilencer and enhancer elements of mouse Tbeta4 promoter
Study
mousen: not reported in source
transient transfection assay
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
A nuclear protein-bound silencer was located in the region between the -167 and -118 and an enhancer whose effect did not seem to be dependent on protein binding was identified in the downstream (-117 to -88) region. However, neither of these cis-elements affected reporter expression driven by a SV40 promoter.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1601097403
04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Activity measurement
The mouse Tbeta4 promoter functioned well in human colorectal (SW480) and cervical (HeLa) carcinoma cells.
In plain wordsIn human cancer cells, the mouse Tbeta4 gene promoter worked well.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableMouse Tbeta4 promoter function in human carcinoma cells
Study
humann: not reported in source
human colorectal (SW480) and cervical (HeLa) carcinoma cells
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
Intriguingly, mTbeta4 promoter functioned well in human colorectal (SW480) and cervical (HeLa) carcinoma cells.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1601097404

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

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