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Study · Not a test of the peptide alone · 2005

7 results

Molecular mechanism of the intracellular segments of the melanocortin-4 receptor for NDP-MSH signaling.

Yang Y, Chen M, Loux TJ, Georgeson KE, Harmon CM

Biochemistry1 May 2005Cited by 9 (Europe PMC)

PMID15865442 (opens in a new tab)DOI10.1021/bi047521+ (opens in a new tab)PepBankPUB-15865442
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone7

In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan I (afamelanotide).

  1. 01LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism

    In cells expressing hMC4R, deleting the distal 25, 22, 18, 17, 16 or 15 amino acids of the C terminus caused complete loss of NDP-MSH binding.

    In plain wordsIn cells, removing the end of the MC4 receptor tail stopped NDP-MSH from binding to it.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseNDP-MSH binding after large C-terminal deletions
    Study

    n: not reported in source

    cells expressing mutant hMC4R
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Our results indicate that deletions of the distal 25 (the entire C terminus), 22, 18, 17, 16, and 15 amino acids of the C terminus result in the complete loss of both [Nle(4)-d-Phe(7)]-alpha-melanocyte stimulating hormone (NDP-MSH) binding and NDP-MSH-mediated cAMP production.
    1 context sentence
    Summary · context
    In this study, we utilized deletions and mutations of specific portions of the hMC4R to determine the molecular mechanism of both the C terminus and the third intracellular loop in receptor signaling.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  2. 02LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism

    In cells expressing hMC4R, deleting the distal 25, 22, 18, 17, 16 or 15 amino acids of the C terminus caused complete loss of NDP-MSH-mediated cAMP production.

    In plain wordsIn cells, removing the end of the MC4 receptor tail stopped NDP-MSH from raising cAMP.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseNDP-MSH-mediated cAMP production after large C-terminal deletions
    Study

    n: not reported in source

    cells expressing mutant hMC4R
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Our results indicate that deletions of the distal 25 (the entire C terminus), 22, 18, 17, 16, and 15 amino acids of the C terminus result in the complete loss of both [Nle(4)-d-Phe(7)]-alpha-melanocyte stimulating hormone (NDP-MSH) binding and NDP-MSH-mediated cAMP production.
    1 context sentence
    Summary · context
    In this study, we utilized deletions and mutations of specific portions of the hMC4R to determine the molecular mechanism of both the C terminus and the third intracellular loop in receptor signaling.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  3. 03LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism

    In cells expressing hMC4R, deleting the distal 14 amino acids of the C terminus significantly decreased NDP-MSH binding affinity and potency.

    In plain wordsIn cells, a slightly smaller deletion of the MC4 receptor tail weakened NDP-MSH binding and potency.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseNDP-MSH binding affinity and potency after deletion of the distal 14 amino acids
    Study

    n: not reported in source

    cells expressing mutant hMC4R
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Deletion of the distal 14 amino acids of the C terminus significantly decreases both NDP-MSH binding affinity and potency
    1 context sentence
    Summary · context
    In this study, we utilized deletions and mutations of specific portions of the hMC4R to determine the molecular mechanism of both the C terminus and the third intracellular loop in receptor signaling.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  4. 04LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism

    In cells expressing hMC4R, deleting the distal 13 amino acids of the C terminus did not affect NDP-MSH activity.

    In plain wordsIn cells, removing the last 13 amino acids of the MC4 receptor tail did not change NDP-MSH activity.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeNDP-MSH activity after deletion of the distal 13 amino acids
    Study

    n: not reported in source

    cells expressing mutant hMC4R
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    but deletion of the distal 13 amino acids of the C terminus does not affect NDP-MSH activity.
    1 context sentence
    Summary · context
    In this study, we utilized deletions and mutations of specific portions of the hMC4R to determine the molecular mechanism of both the C terminus and the third intracellular loop in receptor signaling.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  5. 05LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism

    In cells expressing hMC4R, the proximal 12 amino acids of the C terminus were important for both receptor signaling and ligand binding.

    In plain wordsIn cells, the part of the MC4 receptor tail nearest the cell membrane matters for both signaling and ligand binding.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableRole of the proximal C-terminal region in signaling and ligand binding
    Study

    n: not reported in source

    cells expressing mutant hMC4R
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Further analysis revealed that the proximal 12 amino acids of the C terminus are not only important for receptor signaling but also important for ligand binding.
    1 context sentence
    Summary · context
    In this study, we utilized deletions and mutations of specific portions of the hMC4R to determine the molecular mechanism of both the C terminus and the third intracellular loop in receptor signaling.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  6. 06LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism

    In cells expressing hMC4R, the third intracellular loop was important for receptor signaling.

    In plain wordsIn cells, a loop inside the MC4 receptor is important for the receptor to signal.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableRole of the third intracellular loop in receptor signaling
    Study

    n: not reported in source

    cells expressing mutant hMC4R
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Our results also indicate that the third intracellular loop of the hMC4R is important for receptor signaling but not ligand binding.
    1 context sentence
    Summary · context
    In this study, we utilized deletions and mutations of specific portions of the hMC4R to determine the molecular mechanism of both the C terminus and the third intracellular loop in receptor signaling.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  7. 07LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism

    In cells expressing hMC4R, the third intracellular loop was not important for ligand binding.

    In plain wordsIn cells, a loop inside the MC4 receptor is not important for ligand binding.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeRole of the third intracellular loop in ligand binding
    Study

    n: not reported in source

    cells expressing mutant hMC4R
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Our results also indicate that the third intracellular loop of the hMC4R is important for receptor signaling but not ligand binding.
    1 context sentence
    Summary · context
    In this study, we utilized deletions and mutations of specific portions of the hMC4R to determine the molecular mechanism of both the C terminus and the third intracellular loop in receptor signaling.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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