Skip to content

Study · In vitro (cell-free) · 2005

2 results

Co-operative regulation of ligand binding to melanocortin receptor subtypes: evidence for interacting binding sites.

Kopanchuk S, Veiksina S, Petrovska R, Mutule I, Szardenings M, Rinken A, Wikberg JE

European journal of pharmacology1 Apr 2005Cited by 28 (Europe PMC)

PMID15840392 (opens in a new tab)DOI10.1016/j.ejphar.2005.02.021 (opens in a new tab)PepBankPUB-15840392
Publisher pagePubMedAsk about this study
Jump to 2 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cell-free)Activity measurement

    In insect cell membranes with melanocortin receptors MC1, MC3, MC4 and MC5, specific binding of [125I]NDP-MSH required Ca2+.

    In plain wordsIn lab tests, NDP-MSH needed calcium to bind to melanocortin receptors.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableCalcium dependence of NDP-MSH binding
    Study

    n: not reported in source

    insect cell membranes with baculovirus-expressed melanocortin receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The presence of Ca2+ was found to be mandatory to achieve specific [125I]NDP-MSH binding to the melanocortin receptors.
    1 context sentence
    Summary · context
    This study evaluates the binding the melanocyte stimulating hormone peptide analogue [125I]NDP-MSH to melanocortin receptors MC1, MC3, MC4 and MC5 in insect cell membranes produced by baculovirus expression systems.
  2. 02LabIn vitro (cell-free)Activity measurement

    In insect cell membranes with melanocortin receptors, association of [125I]NDP-MSH followed simple bimolecular kinetics, but its dissociation from the receptors was heterogeneous.

    In plain wordsIn lab tests, NDP-MSH came off melanocortin receptors in an uneven way, although it attached in a simple way.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableDissociation kinetics of NDP-MSH
    Study

    n: not reported in source

    insect cell membranes with baculovirus-expressed melanocortin receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Although association kinetics of [125I]NDP-MSH followed the regularities of simple bimolecular reactions, the dissociation of [125I]NDP-MSH from the melanocortin receptors was heterogeneous.
    1 context sentence
    Summary · context
    This study evaluates the binding the melanocyte stimulating hormone peptide analogue [125I]NDP-MSH to melanocortin receptors MC1, MC3, MC4 and MC5 in insect cell membranes produced by baculovirus expression systems.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.