In a luciferase reporter assay, siRNAs directed against Tbeta4 silenced Tbeta4, did not silence the homologue Tbeta10 off target, and partly knocked down Tbeta10 despite sequence mismatch.
In plain wordsIn lab tests, a reporter assay measured how well siRNAs silence the Tbeta4 gene and whether they also hit a related gene.
- Peptide
- Thymosin beta-4 (human)
- Outcome
- Not applicablesiRNA efficacy and specificity against Tbeta4 and Tbeta10
- Study
n: not reported in source
luciferase reporter assay- Outcome group
- Receptor activity and mechanism · Pharmacology
Source sentences
We have tested different siRNAs directed against Thymosin beta4 (Tbeta4); determined their effectiveness at silencing Tbeta4 and have both excluded off-target silencing of the Tbeta4 homologue Thymosin beta10 (Tbeta10) and demonstrated partial knockdown of Tbeta10 despite significant (12/23; 52%) sequence mismatch.
1 context sentence(design, population, supporting numbers)
To this end, we have adapted a reporter-based assay harnessing the sensitivity of luciferase activity to provide a quantitative readout of relative RNAi efficacy and specificity.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).