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Study · Ex vivo tissue · mouse · 2005

6 results

Cytoprotective peptide humanin binds and inhibits proapoptotic Bcl-2/Bax family protein BimEL.

Luciano F, Zhai D, Zhu X, Bailly-Maitre B, Ricci JE, Satterthwait AC, Reed JC

The Journal of biological chemistry20 Jan 2005Cited by 92 (Europe PMC)

PMID15661735 (opens in a new tab)DOI10.1074/jbc.m413062200 (opens in a new tab)PepBankPUB-15661735
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Summary

Humanin (HN) is a recently identified endogenous peptide that protects cells against cytotoxicity induced by various stimuli. Recently, we showed that HN binds to and inhibits Bax, a proapoptotic Bcl-2 family protein, suggesting a mechanism for HN action. In this study, we identified Bim, a Bcl-2 homology 3-only member of the Bcl-2/Bax family, as an additional HN target protein. Using in vitro protein binding, immunoprecipitation, and coimmunolocalization assays, we demonstrated that HN binds directly to the extra long isoform of Bim (BimEL) but not the long (BimL) or short (BimS) isoforms. HN also protects cells against apoptosis induced by BimEL but not BimL and BimS in gene transfection studies. In contrast, mutants of HN which failed to bind BimEL failed to protect from BimEL-induced cell death. Moreover, HN inhibited BimEL-induced release of SMAC and cytochrome c from mitochondria isolated from bax-/-cells, indicating that HN can suppress BimEL independently of its effect on Bax. Finally, we demonstrate that HN prevents BimEL-induced oligomerization of Bak using isolated mitochondria. Taken together, our results indicate that the inhibition of BimEL may contribute to the antiapoptotic properties of the HN peptide.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueMechanism

    In mitochondria isolated from bax-/- cells, humanin inhibited BimEL-induced release of SMAC, independently of its effect on Bax.

    In plain wordsIn lab tests on isolated mitochondria, humanin reduced the release of SMAC, a cell-death signal, caused by BimEL.

    Peptide
    Humanin
    Outcome
    DecreaseBimEL-induced SMAC release from mitochondria
    Study

    n: not reported in source

    mitochondria isolated from bax-/- cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, HN inhibited BimEL-induced release of SMAC and cytochrome c from mitochondria isolated from bax-/-cells, indicating that HN can suppress BimEL independently of its effect on Bax.
  2. 02LabEx vivo tissueMechanism

    In mitochondria isolated from bax-/- cells, humanin inhibited BimEL-induced release of cytochrome c, independently of its effect on Bax.

    In plain wordsIn lab tests on isolated mitochondria, humanin reduced the release of cytochrome c, a cell-death signal, caused by BimEL.

    Peptide
    Humanin
    Outcome
    DecreaseBimEL-induced cytochrome c release from mitochondria
    Study

    mousen: not reported in source

    mitochondria isolated from bax-/- cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, HN inhibited BimEL-induced release of SMAC and cytochrome c from mitochondria isolated from bax-/-cells, indicating that HN can suppress BimEL independently of its effect on Bax.
  3. 03LabEx vivo tissueMechanism

    In isolated mitochondria, humanin prevented BimEL-induced oligomerization of Bak.

    In plain wordsIn lab tests on isolated mitochondria, humanin stopped BimEL from causing Bak proteins to clump together.

    Peptide
    Humanin
    Outcome
    DecreaseBimEL-induced Bak oligomerization
    Study

    n: not reported in source

    isolated mitochondria
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Finally, we demonstrate that HN prevents BimEL-induced oligomerization of Bak using isolated mitochondria.
  4. 04LabIn vitro (cell-free)Mechanism

    In protein binding assays, humanin bound directly to the extra long isoform of Bim (BimEL) but not to the long (BimL) or short (BimS) isoforms.

    In plain wordsIn lab tests, humanin attached to one form of the cell-death protein Bim (BimEL) but not to two other forms.

    Peptide
    Humanin
    Outcome
    Not applicableHN binding to Bim isoforms
    Study

    n: not reported in source

    in vitro protein binding, immunoprecipitation and coimmunolocalization
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    we demonstrated that HN binds directly to the extra long isoform of Bim (BimEL) but not the long (BimL) or short (BimS) isoforms.

Studies that did not test the peptide alone2

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 05LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In gene transfection studies in cells, humanin protected cells against apoptosis induced by BimEL but not by BimL or BimS.

    In plain wordsIn cells where genes were transferred, humanin protected against cell death caused by BimEL but not by BimL or BimS.

    Peptide
    Humanin
    Outcome
    DecreaseProtection against BimEL-induced apoptosis (gene transfection)
    Study

    n: not reported in source

    Outcome group
    Neuroprotection · Pharmacology

    Source sentences

    Summary
    HN also protects cells against apoptosis induced by BimEL but not BimL and BimS in gene transfection studies.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  2. 06LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In cells, mutants of humanin that failed to bind BimEL failed to protect from BimEL-induced cell death.

    In plain wordsIn cells, changed versions of humanin that could not bind BimEL did not protect against BimEL-induced cell death.

    Peptide
    Humanin
    Outcome
    No significant changeProtection by HN mutants that fail to bind BimEL
    Study

    n: not reported in source

    Outcome group
    Neuroprotection · Pharmacology

    Source sentences

    Summary
    In contrast, mutants of HN which failed to bind BimEL failed to protect from BimEL-induced cell death.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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