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Study · In vitro (cell-free) · 2004

6 results

Interaction of the spectrin-like repeats of alpha-actinin-4 with humanin peptide.

Kigawa A, Wakui H, Maki N, Okuyama S, Masai R, Ohtani H, Komatsuda A, Suzuki D, Toyoda M, Kobayashi R, Sawada K

Clinical and experimental nephrology1 Dec 2004Cited by 8 (Europe PMC)

PMID15619032 (opens in a new tab)DOI10.1007/s10157-004-0322-y (opens in a new tab)PepBankPUB-15619032
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cell-free)Mechanism

    In vitro affinity assays showed specific interactions of humanin-Sepharose with recombinant actinin-4 R1-R4, R1, R2, R3 and R4 proteins.

    In plain wordsIn lab tests, humanin bound to parts of actinin-4 in a specific way.

    Peptide
    Humanin
    Outcome
    Not applicableBinding of humanin to actinin-4 repeats
    Study

    n: not reported in source

    recombinant actinin-4 R1-R4, R1, R2, R3 and R4 proteins with humanin-Sepharose
    Outcome group
    Receptor activity and mechanism · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vitro affinity assays showed specific interactions of recombinant actinin-4 R1-R4, R1, R2, R3, and R4 proteins with humanin-Sepharose.

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 02LabEndogenous levelEx vivo tissueMechanism

    In situ hybridization and immunohistochemistry showed expression of humanin in podocytes.

    In plain wordsTests on tissue showed that podocytes make the body's own humanin.

    Peptide
    Humanin
    Outcome
    Not applicableHumanin expression in podocytes
    Study

    n: not reported in source

    podocytes (in situ hybridization and immunohistochemistry)
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Source sentences

    Summary
    In situ hybridization and immunohistochemistry showed the expression of humanin in podocytes.
    1 context sentence
    Summary · context
    To investigate the expression of the identified molecules in podocytes, in situ hybridization, and immunohistochemical studies were performed.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

Studies that did not test the peptide alone4

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 03LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In a bacterial two-hybrid screen of a human kidney cDNA library with actinin-4 R1-R4 as bait, one isolated cDNA encoded humanin.

    In plain wordsIn a lab screen of human kidney genes, humanin was found as a molecule that binds a part of actinin-4.

    Peptide
    Humanin
    Outcome
    Not applicableHumanin identified as actinin-4 R1-R4 interacting molecule
    Study

    humann: not reported in source

    bacterial two-hybrid screen of human kidney cDNA library
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Source sentences

    Summary
    One isolated cDNA from the library encoded humanin, a recently identified antiapoptotic peptide.
    1 context sentence
    Summary · context
    To identify such molecules, we performed a bacterial two-hybrid screening of a human kidney cDNA library using as a bait human actinin-4 R1-R4.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  2. 04LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In HEK293 cells, green fluorescent protein-fused humanin and endogenous actinin colocalized mainly in the perinuclear cytoplasm.

    In plain wordsIn human kidney-derived cells, tagged humanin and the cell's own actinin sat in the same place near the nucleus.

    Peptide
    Humanin
    Outcome
    Not applicableColocalization of GFP-humanin and endogenous actinin
    Study

    humann: not reported in source

    HEK293 cells with GFP-fused humanin
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Source sentences

    Summary
    Green fluorescent protein-fused humanin and endogenous actinin colocalized mainly in the perinuclear cytoplasm of HEK293 cells.
    1 context sentence
    Summary · context
    We further verified the identified interactions by in vitro affinity assays and immunofluorescent studies of cultured human embryonic kidney HEK293 cells.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  3. 05LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In HEK293 cells, adding puromycin aminonucleoside did not alter the colocalization of GFP-fused humanin and actinin.

    In plain wordsIn human kidney-derived cells, puromycin aminonucleoside did not change where tagged humanin and actinin sat.

    Peptide
    Humanin
    Outcome
    No significant changeEffect of puromycin aminonucleoside on humanin-actinin colocalization
    Study

    humann: not reported in source

    HEK293 cells with GFP-fused humanin
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Source sentences

    Summary
    Altered colocalization was not observed by the addition of PAN.
    1 context sentence
    Summary · context
    Green fluorescent protein-fused humanin and endogenous actinin colocalized mainly in the perinuclear cytoplasm of HEK293 cells.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  4. 06LabNot a test of Humanin aloneIn vitro (cell-free)Mechanism

    Puromycin aminonucleoside (PAN) had no effect on the in vitro interactions of humanin with actinin-4 repeats.

    In plain wordsIn lab tests, puromycin aminonucleoside did not change how humanin bound to actinin-4.

    Peptide
    Humanin
    Outcome
    No significant changeEffect of puromycin aminonucleoside on humanin-actinin-4 binding
    Study

    n: not reported in source

    recombinant actinin-4 repeats with humanin-Sepharose
    Outcome group
    Receptor activity and mechanism · Kidney and urinary

    Source sentences

    Summary
    PAN had no effect on these interactions.
    1 context sentence
    Summary · context
    In vitro affinity assays showed specific interactions of recombinant actinin-4 R1-R4, R1, R2, R3, and R4 proteins with humanin-Sepharose.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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