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Study · In vitro (cells) · black seabream · 2004

5 results

Stimulation of growth hormone secretion from seabream pituitary cells in primary culture by growth hormone secretagogues is independent of growth hormone transcription.

Chan CB, Fung CK, Fung W, Tse MC, Cheng CH

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP1 Oct 2004Cited by 6 (Europe PMC)

PMID15556068 (opens in a new tab)DOI10.1016/j.cca.2004.09.008 (opens in a new tab)PepBankPUB-15556068
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In primary seabream pituitary cells, ipamorelin stimulated growth hormone release in a dose-dependent manner, with EC50 values in the nanomolar range.

    In plain wordsIn cells from seabream pituitary, ipamorelin increased growth hormone release in a dose-dependent way.

    Peptide
    Ipamorelin
    Outcome
    IncreaseGrowth hormone release
    Study

    black seabreamn: not reported in source

    primary culture of pituitary cells
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    A dose-dependent stimulation of GH release was demonstrated by all the GHS tested, except human ghrelin, with EC(50) values in the nanomolar range.
    2 context sentences
    Summary · context
    The peptide GHS employed included growth hormone-releasing peptide (GHRP)-2, ipamorelin, and human ghrelin.
    Summary · context
    The action of a number of growth hormone secretagogues (GHS) on growth hormone (GH) secretion and gene expression was studied in a primary culture of pituitary cells isolated from the black seabream Acanthopagrus schlegeli.

Studies that did not test the peptide alone4

In this study, the peptide was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking the peptide.

  1. 02LabNot a test of Ipamorelin aloneIn vitro (cells)Mechanism

    In primary seabream pituitary cells, growth hormone secretagogues including ipamorelin did not change growth hormone gene expression, even with stimulation up to 48 h.

    In plain wordsIn cells from seabream pituitary, ipamorelin raised growth hormone release without changing the growth hormone gene's activity.

    Peptide
    Ipamorelin
    Outcome
    No significant changeGrowth hormone mRNA level
    Study

    black seabreamn: not reported in source

    primary culture of pituitary cells
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    Simultaneous measurement of GH mRNA levels in the incubated seabream pituitary cells indicated that the GHS-stimulated increase in GH secretion was not paralleled by corresponding changes in GH gene expression.
    3 context sentences
    Summary · context
    no change in GH gene expression was noticed in the seabream pituitary cells even though the time of stimulation by GHS was increased up to 48 h
    Summary · context
    The peptide GHS employed included growth hormone-releasing peptide (GHRP)-2, ipamorelin, and human ghrelin.
    Summary · context
    The action of a number of growth hormone secretagogues (GHS) on growth hormone (GH) secretion and gene expression was studied in a primary culture of pituitary cells isolated from the black seabream Acanthopagrus schlegeli.

    In this study, Ipamorelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Ipamorelin.

  2. 03LabNot a test of GHRP-2 aloneIn vitro (cells)Activity measurement

    In primary black seabream pituitary cells, the GH secretagogues tested, including GHRP‑2, stimulated GH release dose-dependently, except human ghrelin.

    In plain wordsIn lab tests on seabream pituitary cells, growth hormone releasing compounds, GHRP‑2 among them, raised growth hormone release. Human ghrelin did not.

    Peptide
    GHRP-2
    Outcome
    IncreaseGH release from pituitary cells
    Study

    black seabream (Acanthopagrus schlegeli)n: not reported in source

    Primary pituitary cell culture
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    A dose-dependent stimulation of GH release was demonstrated by all the GHS tested, except human ghrelin, with EC(50) values in the nanomolar range.
    1 context sentence
    Summary · context
    The action of a number of growth hormone secretagogues (GHS) on growth hormone (GH) secretion and gene expression was studied in a primary culture of pituitary cells isolated from the black seabream Acanthopagrus schlegeli.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  3. 04LabNot a test of GHRP-2 aloneIn vitro (cells)Activity measurement

    In primary black seabream pituitary cells, GH secretagogue-stimulated GH secretion was not paralleled by changes in GH gene expression.

    In plain wordsIn lab tests on seabream pituitary cells, more growth hormone was released without a matching change in the growth hormone gene signal.

    Peptide
    GHRP-2
    Outcome
    No significant changeGH mRNA level (GH gene expression)
    Study

    black seabream (Acanthopagrus schlegeli)n: not reported in source

    Primary pituitary cell culture
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    Simultaneous measurement of GH mRNA levels in the incubated seabream pituitary cells indicated that the GHS-stimulated increase in GH secretion was not paralleled by corresponding changes in GH gene expression.
    1 context sentence
    Summary · context
    The action of a number of growth hormone secretagogues (GHS) on growth hormone (GH) secretion and gene expression was studied in a primary culture of pituitary cells isolated from the black seabream Acanthopagrus schlegeli.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  4. 05LabNot a test of GHRP-2 aloneIn vitro (cells)Activity measurement

    In primary black seabream pituitary cells, GH gene expression did not change even when stimulation by the secretagogues lasted up to 48 h.

    In plain wordsIn lab tests on seabream pituitary cells, the growth hormone gene signal stayed unchanged even after 48 hours of stimulation.

    Peptide
    GHRP-2
    Outcome
    No significant changeGH gene expression up to 48 h of stimulation
    Study

    black seabream (Acanthopagrus schlegeli)n: not reported in source

    Primary pituitary cell culture
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    no change in GH gene expression was noticed in the seabream pituitary cells even though the time of stimulation by GHS was increased up to 48 h
    1 context sentence
    Summary · context
    The action of a number of growth hormone secretagogues (GHS) on growth hormone (GH) secretion and gene expression was studied in a primary culture of pituitary cells isolated from the black seabream Acanthopagrus schlegeli.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Versions 4 to 18Current

    28 Oct 2026, 02:02 UTC

    The same notice was added 15 times, first on 13 Oct 2026, 21:44 UTC. The status did not change between them.

    Study results updated

  2. Version 3

    13 Oct 2026, 16:31 UTC

    Study data updated · source update from Europe PMC

  3. Version 2

    9 Oct 2026, 18:17 UTC

    Study results updated

  4. Version 1

    9 Oct 2026, 03:13 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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